Nanog expression in mouse germ cell development
Section snippets
Results and discussion
Germ cells are specialized for handing down the parent's genetic information to posterity. For this purpose, drastic genetic and epigenetic changes occur through molecular events such as nuclear reprogramming, meiosis and transformation to the sperm or oocyte. In the mouse, primordial germ cells (PGCs) are first identified as alkaline phosphatase-positive cells clustering in the proximal region of the allantois of the E7.25 embryo (Ginsburg et al., 1990). It has been recently proposed that germ
Mouse embryos and gonads
Mice were maintained in a controlled environment with a 12-h light/dark cycle (light on; 8:00 am to 8:00 pm). E7.5–E16.5 embryos and their developing gonads were obtained from C57BL/6J females mated with C57BL/6J males. Developmental stages of embryos were determined on the basis of morphological features and the pair number of somites. For the induction of oocyte maturation, 7.5 IU of PMSG (Teikoku Hormone) was intraperitoneally injected into 8-week-old female mice. Ovaries were collected 44 h
Acknowledgements
We thank Dr Toru Nakano for the anti-PGC7 antibody, Dr Yoshitake Nishimune for the anti-TRA98 antibody, Dr Shinichiro Chuma for the SCP3 antibody and Miss Emiko Moribe for technical assistance with the breeding of mice.
References (20)
- et al.
Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells
Cell
(2003) - et al.
Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling
Dev. Biol.
(2001) - et al.
Pluripotential competence of cells associated with Nanog activity
Mech. Dev.
(2005) - et al.
Generation of pluripotent stem cells from neonatal mouse testis
Cell
(2004) - et al.
Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture
Cell
(1992) - et al.
The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
Cell
(2003) - et al.
Identification of PGC7, a new gene expressed specifically in preimplantation embryos and germ cells
Mech. Dev.
(2002) - et al.
A novel NK-type homeobox gene, ENK (early embryo specific NK), preferentially expressed in embryonic stem cells
Gene Expr. Patterns
(2003) - et al.
Multipotent cell lineages in early mouse development depend on SOX2 function
Genes Dev.
(2003) - et al.
Primordial germ cells in the mouse embryo during gastrulation
Development
(1990)
Cited by (245)
A short history of pluripotent stem cells markers
2024, Stem Cell ReportsCharacterization of nanog in Nile tilapia (Oreochromis niloticus) and its spatiotemporal expression patterns during embryonic and gonadal development
2022, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular BiologyBacteria to form induced pluripotent stem cells
2021, Methods in iPSC TechnologyMale germ cell derivation from PSCs
2021, Recent Advances in iPSC-Derived Cell Types: Volume 4 in Advances in Stem Cell BiologyTET1 Interacts Directly with NANOG via Independent Domains Containing Hydrophobic and Aromatic Residues
2020, Journal of Molecular Biology