Neuroprotective effects of the cyanidin-3-O-β-d-glucopyranoside isolated from mulberry fruit against cerebral ischemia

doi:10.1016/j.neulet.2005.08.053

Neuroscience Letters

Volume 391, Issue 3, 2 January 2006, Pages 122-126

https://doi.org/10.1016/j.neulet.2005.08.053 Get rights and content

Abstract

This study investigated the neuroprotective effects of cyanidin-3-O-β-d-glucopyranoside (C3G) from the mulberry fruits on neuronal cell damage. A 1% HCl–MeOH mulberry fruit extract was shown to have a cytoprotective effect on PC12 cells that had been exposed to hydrogen peroxide. The extract inhibited the cerebral ischemic damage caused by oxygen glucose deprivation (OGD) in PC12 cells. The neuroprotective effect of the mulberry fruit extract was further demonstrated in vivo using a mouse-brain-injury model with a transient middle cerebral artery occlusion (MCAO). C3G was isolated as a neuroprotective constituent from the mulberry fruit extract. Compared with the control group, C3G had neuroprotective effects on the PC12 cells exposed to hydrogen peroxide in vitro and on cerebral ischemic damage in vivo.

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Acknowledgments

This work was supported by the grants of Brain Korea 21 projects (Ministry of Education, Korea) and by the grant (PF0320601-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by the Ministry of Science and technology of Korean government.

References (14)

J. Chandra et al.
Triggering and modulation of apoptosis by oxidative stress
Free Radic. Biol. Med.
(2000)
P.J. Crack et al.
Reactive oxygen species and the modulation of stroke
Free Radic. Biol. Med.
(2005)
M.B. Hansen et al.
Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
J. Immunol. Methods
(1989)
N.P. Seeram et al.
Cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries
Phytomedicine
(2001)
I. Serraino et al.
Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure
Life Sci.
(2003)
J. Tian et al.
Neuroprotective effect of 20(S)-ginsenoside Rg3 on cerebral ischemia in rats
Neurosci. Lett.
(2005)
N. Asano et al.
Polyhydroxylated alkaloids isolated from mulberry trees (Morusalba L.) and silkworms (Bombyx mori L.)
J. Agric. Food Chem.
(2001)

There are more references available in the full text version of this article.

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Neuroprotective effects of the cyanidin-3-O-β-d-glucopyranoside isolated from mulberry fruit against cerebral ischemia

Abstract

Section snippets

Acknowledgments

Free Radic. Biol. Med.

Free Radic. Biol. Med.

J. Immunol. Methods

Phytomedicine

Life Sci.

Neurosci. Lett.

Polyhydroxylated alkaloids isolated from mulberry trees (Morusalba L.) and silkworms (Bombyx mori L.)

J. Agric. Food Chem.