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Sample size, power and effect size – What all researchers need to know

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    The investigators performing data analysis were blinded to group allocations and experimental treatment. The required experimental sample size and archived power were calculated based on the preliminary experiments by using G*Power 3.1.5 software (α = .05, power = 0.848).29 Bronchoalveolar lavage and lung digestion were performed in the experimental animals, as described previously,30 and cells were used for the preparation of cytospins and flow cytometry.

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    After euthanizing these mice, tumors weighing more than 700 mg were used for the immunohistochemistry and flow cytometry based investigation. Sample size calculations were performed using the DSS sample size calculator (DSS Research) as described elsewhere [54,55]. Three mice per experiment/group were determined as optimum sample size using DSS sample size calculator to get statistically significance between experimental groups [54,55].

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