Experimental radiotherapyTumor lactate content predicts for response to fractionated irradiation of human squamous cell carcinomas in nude mice☆
Section snippets
Animals and tumor models
The study was performed using five lines of human squamous cell carcinomas (HNSCC) of the head and neck region xenografted in nude mice. Details of the animals, tumor lines and the local tumor control assay are described in Yaromina et al., this issue. In brief, tumors were transplanted subcutaneously into the right hind leg of nude mice whole body irradiated with 4 Gy (200 kV X-rays, 0.5 mm Cu filter) 2 days before transplantation. Tumors were either excised for histological studies and
Results
The process of structure-associated metabolic imaging is illustrated by Fig. 1 showing sequential cryosections through a human HNSCC (UT-SCC-15) xenograft representing the color-coded concentration distribution of lactate (a), the anti-mouse staining (b) and the hematoxylin and eosin (H&E) staining (c). The anti-mouse staining allows for an unequivocal discrimination between the human tumor xenograft (unstained) and the host murine tissue (dark brown staining). Using interactive image analysis,
Discussion
Structure-associated and quantitative metabolic imaging with bioluminescence is a unique technology for the assessment of quantitative information regarding the metabolic status of selected tissue areas. This is important for studies on solid tumors, since most cancers are heterogeneous containing viable tumor regions, more or less extended necrosis, stromal elements and a variable proportion of infiltrated immune cells. The current study has been focussed on the interaction between radiation
Acknowledgements
The authors thank Mrs. E. Wenzel, Mrs. S. Balschukat, Mrs. D. Pfitzmann, Mrs. K. Schumann and Mrs. M. Oelsner for excellent technical assistance.
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Financial support: This investigation was supported by grants of the Deutsche Forschungsgemeinschaft to W.M.K. (Mu 576/14-1) and to M.B. and D.Z. (Ba 1433/4-2).
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Shared first authorship.
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Shared senior authorship.