Molecular detection of porcine kobuviruses in Italian swine
Section snippets
Conflict of interest statement
All Authors declare that there are no financial or other relationships that might lead to a conflict of interest. All authors have seen and approved the manuscript and have contributed significantly to the work.
Acknowledgements
The authors wish to thank Dr Claudia Costanzo for the collection of fecal specimens. The work was financed by grants from the University of Teramo, Italy, and from the Italian Ministry of University and Research.
References (37)
- et al.
First detection of kobuvirus in farm animals in Brazil and the Netherlands
Infection, Genetic and Evolution
(2011) - et al.
Molecular detection of kobuvirus sequences in stool samples collected from healthy pigs in Japan
Infection, Genetics and Evolution
(2010) - et al.
Molecular detection and genetic characterization of kobuviruses in fecal samples collected from diarrheic cattle in Korea
Infection, Genetics and Evolution
(2011) - et al.
Prevalence and genetic diversity of aichi virus strains in stool samples from community and hospitalized patients
Journal of Clinical Microbiology
(2008) - et al.
Porcine kobuvirus from pig stool in Korea
Virus Genes
(2011) - et al.
Complete genome sequence of the porcine kobuvirus variant CH/HNXX-4/2012
Journal of Virology
(2012) - et al.
Molecular detection of bovine kobuviruses in Italy
Archives of Virology
(2012) - et al.
The complete genome of klassevirus – a novel picornavirus in pediatric stool
Virology Journal
(2009) - et al.
Klassevirus 1, a previously undescribed member of the family Picornaviridae, is globally widespread
Virology Journal
(2009) - et al.
Three clusters of bovine kobuvirus isolated in Korea, 2008–2010
Virus Genes
(2011)
Characterization of a canine homolog of human aichivirus
Journal of Virology
Porcine kobuvirus in piglets, Thailand
Emerging Infectious Diseases
Bovine kobuviruses from cattle with diarrhea
Emerging Infectious Diseases
Picornaviridae
MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment
Briefing in Bioinformatics
Kobuvirus in South Korean black goats
Virus Genes
Complete genome sequence of porcine kobuvirus strain WUH1
Journal Virology
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2019, Veterinary MicrobiologyCitation Excerpt :Since those cell cultures are only used to study human kobuviruses, a new cell culture protocol capable of isolating the porcine kobuvirus is needed to evaluate the viability of porcine kobuviruses. To assess the phylogenetic differences found between the samples in this study, positive Kobuvirus samples identified using the “universal” primer pair were subjected to amplification tests with different primer pairs targeting the more variable VP1 segment, in the same manner as other studies in Europe and Asia (Yu et al., 2011; Di Profio et al., 2013). The unsuccessful attempts to amplify the VP1 with European and Asian primer pairs, as previously documented in a study conducted in Minnesota, USA (Verma et al., 2013), led to the design of a new set of primers amplifying a longer fragment.
Porcine kobuvirus 1 in healthy and diarrheic pigs: Genetic detection and characterization of virus and co-infection with rotavirus A
2017, Infection, Genetics and EvolutionCitation Excerpt :Surprisingly, they were not clustered near sequences originating from neighbouring countries but rather with Asian or African viral sequences (Fig. 1). Similar phenomenon has been observed with Italian PKV-1 sequences (Di Profio et al., 2013). At present it is difficult to explain this observation since there is not any known trading of pigs or products from/to Slovakia outside Europe.
Development of TaqMan real-time reverse transcription-polymerase chain reaction for the detection and quantitation of porcine kobuvirus
2016, Journal of Virological MethodsCitation Excerpt :PKV was first identified in apparently healthy pigs in Hungary in 2008 (Reuter et al., 2008). Since then, it has been detected in both asymptomatic and diarrheic animals in several other countries, including Japan (Khamrin et al., 2010), China (Yu et al., 2009), South Korea (Park et al., 2010), Thailand (Khamrin et al., 2009), Brazil (Ribeiro et al., 2013), the USA (Verma et al., 2013; Sisay et al., 2013; Liu et al., 2015), and Italy (Di Profio et al., 2013). The genome of PKV is 8.2–8.4 kb in length with a viral protein genome(VPg) linked to the 5′ end, and a poly(A) tail at the 3′ end.
Molecular investigations on the prevalence and viral load of enteric viruses in pigs from five European countries
2016, Veterinary MicrobiologyCitation Excerpt :Still, the influence of PKoV should not be totally neglected. In the present study PKoV was demonstrated at rather equal incidences regardless of age, despite that a decrease of prevalence by age has been recorded in East Africa (Amimo et al., 2014) and in Italy, where the PKoV was detected only in 3.85% out of 280 fecal samples collected from healthy pigs aged between 6 and 10 months (Di Profio et al., 2013). Unlike PKoV, it is established that PCV2 can contribute to several clinical diseases, including potential participation to enteric clinical disorders in nursery and growing-finishing pigs (Baró et al., 2015).