Original articleDuodenal-jejunal bypass attenuates progressive failure of pancreatic islets in streptozotocin-induced diabetic rats
Section snippets
Animals
A cohort of 8-week old male Sprague–Dawley (SD) rats weighing 180–200 g was adopted from the Shanghai Laboratory Animal Research Center (Shanghai, China). The rats were housed in individual cages with controlled temperature (24±2°C), humidity (40%–70%), and light/dark cycle (12 hr light/12 hr dark, with light on at 7:00 a.m.) and allowed unrestricted access to standard rat chow and tap water. The study protocol was reviewed and approved by the Institutional Animal Care and Utilization Committee
Food intake and weight
Food intake and weight of each rat were measured 1 day before and 1 week after the DJB or sham procedure, and 10 and 20 days after STZ administration. At the onset of this experiment, animals were randomized into 3 groups, all of which had a comparable baseline in food intake and weight. Then the DJB or sham procedure was performed to induce hormonal and cytokine changes in rats. One week after surgery, there was no significant difference in food intake among DJB, S, and C groups.
Ten days after
Discussion
In this study, nonobese healthy rats underwent prophylactic DJB to reroute the intestine to induce beneficial biological or hormonal effects. Both rats that had a sham procedure and rats that did not have surgery served as controls. A moderate dosage of STZ then was administered to selectively induce pancreatic beta cell apoptosis in all the rats. After these treatments, we found that the rats that underwent DJB did not experience typical symptoms of uncompensated diabetes, including
Disclosures
The authors have no commercial associations that might be a conflict of interest in relation to this article.
Acknowledgments
The authors wish to thank Zhengdong Qiao, Yueqian Wang, Lili Gao, and Yongjun Liang for assistance with the experiments and technical support and Dan Yang for valuable discussions. This study was supported by Young Medical Talents Training Program of Pudong Health Bureau of Shanghai (Grant No. PWRq2013-14), Shanghai Pujiang Telant Project Grant (No. 14 PJ1407800), Shanghai Commission of Science and Technology Grant (Grant No.124119 b1800), as well as Pudong Bureau of Health and Family Planning
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