Cell Stem Cell
Volume 16, Issue 5, 7 May 2015, Pages 504-516
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Article
Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation

https://doi.org/10.1016/j.stem.2015.03.007Get rights and content
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Highlights

  • The lncRNA Haunt and its genomic locus play distinct roles in HOXA gene activation

  • The Haunt DNA locus contains potential HOXA enhancers

  • Haunt RNA binds to chromatin and acts to prevent aberrant HOXA expression

  • Haunt orchestrates proper ESC differentiation

Summary

Long noncoding RNAs (lncRNAs) have been implicated in controlling various aspects of embryonic stem cell (ESC) biology, although the functions of specific lncRNAs, and the molecular mechanisms through which they act, remain unclear. Here, we demonstrate discrete and opposing roles for the lncRNA transcript Haunt and its genomic locus in regulating the HOXA gene cluster during ESC differentiation. Reducing or enhancing Haunt expression, with minimal disruption of the Haunt locus, led to upregulation or downregulation of HOXA genes, respectively. In contrast, increasingly large genomic deletions within the Haunt locus attenuated HOXA activation. The Haunt DNA locus contains potential enhancers of HOXA activation, whereas Haunt RNA acts to prevent aberrant HOXA expression. This work reveals a multifaceted model of lncRNA-mediated transcriptional regulation of the HOXA cluster, with distinct roles for a lncRNA transcript and its genomic locus, while illustrating the power of rapid CRISPR/Cas9-based genome editing for assigning lncRNA functions.

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