Original articleGene expression profiling in biliary epithelial cells of primary biliary cirrhosis using laser capture microdissection and cDNA microarray
Section snippets
Subjects
Liver specimens were obtained from 5 PBC patients (the PBC group) and 3 CHC patients (the CHC group) by laparoscopic liver biopsy. Liver specimens were also obtained from 3 normal subjects (the normal group), who were living donors of liver transplantation, by intraoperative needle biopsy. All subjects were admitted to Okayama University Hospital of Medicine and Dentistry, and liver biopsy was performed for the diagnosis of liver diseases. The study was conducted in accordance with the
Clinical, serological, and histopathological profile of the subjects
Clinical, serological, and histopathological data of the 5 PBC patients, the 3 CHC patients, and the 3 normal subjects are shown in Table I. Histological diagnoses of the PBC patients were stage 1/2/3 in 1/2/2 patients, respectively, according to Scheuer’s and Ludwig’s classifications.18, 19 Serum liver enzymes, that is, AST, ALT, ALP, and γ-GTP, were elevated in all PBC patients, and T-Bil was elevated in the 2 PBC patients. AMA and AMA-M2 were positive in all PBC patients. ANAs were positive
Discussion
To elucidate the etiology of PBC, it seems necessary to analyze the alterations of gene expression in BEC, the target cells that are mainly disordered in PBC. It was hypothesized that combining recently developed methods—LCM, T7-based RNA amplification, and cDNA microarray—would make it possible to analyze the gene expression in the selectively isolated BEC.
The first method, LCM, enabled identification and selective collection of a particular cell under a microscope. In the authors’ experiment,
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2017, Journal of HepatologyCitation Excerpt :In PBC, differentially expressed miRNAs have been identified in liver tissue, biliary epithelium, serum, and peripheral blood mononuclear cells (PBMCs) [19–22]. In addition, mRNA profiling studies in PBC have also been reported [7,23,24]. However, no studies have yet analysed miRNA or mRNA expression profiles in CD4+ T cells isolated from PBC patients.
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2015, Journal of AutoimmunityCitation Excerpt :Importantly, CD4+ [11] and CD8+ [12] T cells specific to mitochondrial auto-antigens have been demonstrated in the peripheral blood, livers and liver-draining lymph nodes of affected patients, while not detected in either healthy controls or patients with other liver diseases. Both MHC class I and II proteins are also expressed on BECs of PBC patients and thought to present antigen to cytotoxic CD8+ and helper CD4+ T cells, respectively [13–15]. The cytokine signature associated with PBC is also indicative of immune system activation with a Th1/Th17 bias.
Anatomy and Physiology of the Biliary Epithelium
2010, Comprehensive Toxicology, Second Edition