Basic scienceEffect of botulinum toxin on detrusor overactivity induced by intravesical adenosine triphosphate and capsaicin in a rat model
Section snippets
Bladder tube placement
The rats were anesthetized with halothane general anesthesia. The bladders were exposed through a midline incision. A Clay-Adams PE-50 (polyethylene) tube (1 mm outer diameter) was placed through a small incision in the dome of the bladder. The bladder was closed in a purse-string manner. The PE-50 tube was then tunneled under the subcutaneous tissue and exited through the interscapular space. A Covance infusion harness was used to secure the tube. The University of Maryland IUCAC approved all
CMG tracings
Figure 1 depicts representative CMG tracings from the experiments. Figure 1A is of a saline CMG. Figure 1B shows detrusor overactivity in response to intravesical infusion of 20 mM ATP. Figure 1C shows the effect of intravesical 100 μM capsaicin. Representative CMG tracings from animals treated to 1.0 U BTX and then infused with ATP and capsaicin are shown in Figure 1D and Figure 1E, respectively.
Effect of BTX on detrusor overactivity induced by ATP
ATP infusion into the rat bladder significantly increased the frequency of bladder contractions
Comment
The symptom complex of OAB represents both a diagnostic and a treatment dilemma for the urologist. First, it is a diagnosis of exclusion, because no pathognomonic criterion is available to confirm an OAB. Because of this, treatment is empiric and directed at stopping the presumed unwanted bladder contractions. Ideally, a drug should also stop or diminish the sense of bladder urgency. It is along these lines that antimuscarinics, such as oxybutynin and tolterodine, have been developed to treat
References (17)
- et al.
Current and future pharmacological treatment for overactive bladder
J Urol
(2002) - et al.
Intravesical capsaicin and resiniferatoxin therapyspicing up the ways to treat overactive bladder
J Urol
(1999) - et al.
Augmented stretch activated adenosine triphosphate release from bladder uroepithelial cells in patients with interstitial cystitis
J Urol
(2001) - et al.
Effect of doxazosin on stretch-activated adenosine triphosphate release in bladder urothelial cells from patients with benign prostatic hyperplasia
Urology
(2002) - et al.
Intravesical adenosine triphosphate stimulates the micturition reflex in awake, freely moving rats
J Urol
(2002) - et al.
Urodynamic effects induced by intravesical capsaicin in rats and hamsters
Auton Neurosci Basic Clin
(2001) - et al.
Botulinum-A toxin for treating detrusor hyperreflexia in spinal cord injured patientsa new alternative to anticholinergic drugs? Preliminary results
J Urol
(2000) - et al.
Botulinum toxin urethral sphincter injection to restore bladder emptying in men and women with voiding dysfunction
J Urol
(2001)
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This study was supported by a minigrant from the National Kidney Foundation of Maryland.