Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells
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Highlights
► Microfluidic arrays enable analysis of 96 qPCR assays on 1440 single cells. ► Detailed methods on obtaining qPCR data and performing preliminary data processing. ► Data from sufficient cells to address noise inherent in single-cell transcription. ► Methods used for conventional qPCR do not necessarily apply to single-cell qPCR.
Keywords
Single-cell gene expression profiling
High throughput qPCR
Real-time PCR
Microfluidic arrays
Eukaryotic transcription
Stochastic noise in gene expression
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Copyright © 2012 Elsevier Inc.