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Relative developmental and reproductive fitness associated with pyrethroid resistance in the major southern African malaria vector, Anopheles funestus

Published online by Cambridge University Press:  12 November 2007

P.N. Okoye ,
B.D. Brooke ,
R.H. Hunt  and
M. Coetzee
P.N. Okoye
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private Bag X4, Sandringham, 2131, South Africa School of Animal Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa
B.D. Brooke*
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private Bag X4, Sandringham, 2131, South Africa Division of Virology and Communicable Disease Surveillance, School of Pathology of the National Health Laboratory Service and the University of the Witwatersrand, Johannesburg, South Africa
R.H. Hunt
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private Bag X4, Sandringham, 2131, South Africa School of Animal Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa
M. Coetzee
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private Bag X4, Sandringham, 2131, South Africa Division of Virology and Communicable Disease Surveillance, School of Pathology of the National Health Laboratory Service and the University of the Witwatersrand, Johannesburg, South Africa
*
*Author for correspondence Fax: +27 11 386-6481 E-mail: basilb@nicd.ac.za
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Abstract

The effect of pyrethroid resistance on the fitness of a laboratory strain of Anopheles funestus originating from southern Mozambique was evaluated by comparing the developmental and reproductive characteristics of a pyrethroid resistant strain with an insecticide susceptible strain. Fitness was evaluated in terms of fecundity, fertility, egg production, developmental time and life stage progression and survival. Of the eggs laid by females of the resistant strain, 81.5% hatched while only 66.9% were recorded in the susceptible strain. The time from egg hatch to adult emergence was longer for the resistant strain (15.9 days) than the susceptible strain (15.2 days). A significantly higher proportion of eggs from the resistant strain (61.6%) survived to adulthood compared with those of the susceptible strain (49%). Fecundity and larval and pupal survival did not differ significantly between strains. Of spermathecae dissected from females of the resistant strain, 56.8% were fertilized compared to 52.6% from the susceptible strain. The proportion of females that successfully produced eggs was 43.3% and 23.3% for the resistant and susceptible strains respectively. Complete failure of larval hatch was recorded in 28.6% of susceptible strain families compared to 7.7% of resistant families. Our results show that pyrethroid resistance in southern African An. funestus does not incur any loss of fitness under laboratory conditions. These results suggest that the removal of pyrethroid insecticide selection pressure may not lead to a regression of resistance alleles in pyrethroid resistant An. funestus populations in southern Africa.

Keywords

Anopheles funestusfitnessinsecticide resistancesouthern Africapyrethroidsinsecticide resistance management
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 6 , December 2007 , pp. 599 - 605
Copyright
Copyright © Cambridge University Press 2007

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References

Amenya, D.A., Koekemoer, L.L., Vaughan, A., Morgan, J.C., Brooke, B.D., Hunt, R.H., Ranson, H., Hemingway, J. & Coetzee, M. (2005) Isolation and sequence analysis of P450 genes from a pyrethroid resistant colony of the major malaria vector Anopheles funestus. DNA Sequence 16, 437–445.CrossRefGoogle ScholarPubMed
Amin, A.M. & White, G.B. (1984) Relative fitness of organophosphate-resistant and susceptible strains of Culex quinquefasciatus Say (Diptera: Culicidae). Bulletin of Entomological Research 74, 591–598.CrossRefGoogle Scholar
Armbruster, P., Hutchinson, R.A. & Linvell, T. (2000) Equivalent inbreeding depression under laboratory and field conditions in a tree-hole-breeding mosquito. Proceedings of the Royal Society of London B 267, 1939–1946.CrossRefGoogle Scholar
Bonning, B.C. & Hemingway, J. (1991) Identification of reduced fitness associated with an insecticide resistance gene in Culex pipiens by microtitre plate tests. Medical and Veterinary Entomology 5, 377–379.CrossRefGoogle ScholarPubMed
Brooke, B.D., Kloke, G., Hunt, R.H., Koekemoer, L.L., Temu, E.A. & Taylor, M.E. (2001) Bioassay and biochemical analyses of insecticide resistance in southern African Anopheles funestus (Diptera: Culicidae). Bulletin of Entomological Research 91, 265–272.CrossRefGoogle ScholarPubMed
Campanhola, C., McCutchen, B.F., Baehrecke, E.H. & Plapp, F.W. Jr. (1991) Biological constraints associated with resistance to pyrethroids in the tobacco budworm (Lepidoptera: Noctuidae). Journal of Economic Entomology 84, 1404–1411.CrossRefGoogle Scholar
Clements, A.N. (1992) The Biology of Mosquitoes: Development, Nutrition and Reproduction. 536 pp. London, Chapman and Hall.CrossRefGoogle Scholar
Coetzee, M. (2006) Malaria and dengue vector biology and control in southern and eastern Africa. pp. 101–109in Knols, B.G.J. & Louis, C. (Eds) Bridging Laboratory and Field Research for Genetic Control of Disease Vectors. Berlin, Springer.Google Scholar
Crow, J.F. (1957) Genetics of insecticide resistance to chemicals. Annual Review of Entomology 2, 227–246.CrossRefGoogle Scholar
Ferrari, J.A. & Georghiou, G.P. (1981) Effects on insecticidal selection and treatment on reproductive potential of resistant, susceptible, and heterozygous strains of the southern house mosquito. Journal of Economic Entomology 74, 323–327.CrossRefGoogle Scholar
ffrench-Constant, R.H. & Roush, R.T. (1990) Resistance detection and documentation: the relative roles of pesticidal and biochemical assays. pp. 4–38in Roush, R.T. & Tabashnik, B.E. (Eds) Pesticide Resistance in Arthropods. New York, Chapman and Hall.CrossRefGoogle Scholar
Garros, C., Koekemoer, L.L., Kamau, L., Awolola, T.S., Van Bortel, W., Coetzee, M., Coosemans, M. & Manguin, S. (2004) Restriction fragment length polymorphism method for the identification of major African and Asian malaria vectors within the Anopheles funestus and An. minimus groups. American Journal of Tropical Medicine and Hygiene 70, 260–265.CrossRefGoogle Scholar
Georghiou, G.P. (1983) Management of resistance in arthropods. pp. 769–792in Georghiou, G.P. & Saito, T. (Eds) Pest Resistance to Pesticides. New York, Plenum.CrossRefGoogle Scholar
Gillies, M.T. & Coetzee, M. (1987) A supplement of the Anophelinae of Africa south of the Sahara. Publications of the South African Institute for Medical Research 55, 1–143.Google Scholar
Gillies, M.T. & De Meillon, B. (1968) The Anophelinae of Africa south of the Sahara. Publications of the South African Institute for Medical Research 54, 1–343.Google Scholar
Hargreaves, K., Koekemoer, L.L., Brooke, B.D., Hunt, R.H., Mthembu, J. & Coetzee, M. (2000) Anopheles funestus resistant to pyrethroid insecticides in South Africa. Medical and Veterinary Entomology 14, 181–189.CrossRefGoogle ScholarPubMed
Hargreaves, K., Hunt, R.H., Brooke, B.D., Mthembu, J., Weeto, M.M., Awolola, T.S. & Coetzee, M. (2003) Anopheles arabiensis and An. quadriannulatus resistance to DDT in South Africa. Medical and Veterinary Entomology 17, 417–422.CrossRefGoogle Scholar
Heather, N.W. (1982) Comparison of population growth rates of malathion resistant and susceptible populations of the rice weevil, Sitopilus oryzae (Linnaeus) (Coleoptera: Curculionidae). Queensland Journal of Agricultural and Animal Sciences 39, 61–68.Google Scholar
Hunt, R.H., Brooke, B.D., Pillay, C., Koekemoer, L.L. & Coetzee, M. (2005) Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus. Medical and Veterinary Entomology 19, 271–275.CrossRefGoogle ScholarPubMed
Koekemoer, L.L., Kamau, L., Garros, C., Manguin, S., Hunt, R.H. & Coetzee, M. (2006) Impact of the Rift Valley on restriction fragment length polymorphism typing of the major African malaria vector Anopheles funestus (Diptera: Culicidae). Journal of Medical Entomology 43, 1178–1184.CrossRefGoogle ScholarPubMed
Leeper, J.R., Roush, R.T. & Reynolds, H.T. (1986) Preventing or managing resistance in arthropods. pp. 335–346in National Research Council, Committee on Strategies for the Management of Pesticide Resistance Pest Populations, Pesticide resistance: strategies and tactics for management. Washington, USA, National Academy Press.Google Scholar
May, R.M. & Dobson, A.P. (1986) Population dynamics and the rate of evolution of pesticide resistance. pp. 170–193in National Research Council, Committee on Strategies for the Management of Pesticide Resistance Pest Populations, Pesticide resistance: strategies and tactics for management. Washington, USA, National Academy Press.Google Scholar
Munstermann, L.E. (1994) Unexpected genetic consequences of colonization and inbreeding: allozyme tracking in Culicidae (Diptera). Annals of the Entomological Society of America 87, 157–164.CrossRefGoogle Scholar
Ntomwa, B.N. (2004) Biology and behaviour of malaria mosquitoes and their role in Malaria transmission in northern Namibia. MSc thesis, University of the Witswatersrand, South Africa.Google Scholar
Perkins, B.D. Jr. & Grayson, J.M. (1961) Some biological comparisons of resistant and non-resistant strains of the German cockroach, Blatella germanica. Journal of Economic Entomology 54, 747–750.CrossRefGoogle Scholar
Raymond, M., Berticat, C., Weill, M., Pasteur, N. & Chevillon, C. (2001) Insecticide resistance in the mosquito culex pipiens: what have we learned about adaptation? Genetica 112, 287–296.CrossRefGoogle ScholarPubMed
Reisen, W.K., Milby, M.M. & Bock, M.E. (1984) The effects of immature stress on selected events in the life history of Culex tarsalis. Mosquito News 44, 385–395.Google Scholar
Rodcharoen, J. & Mulla, S.M. (1997) Biological fitness of Culex quinquefasciatus (Diptera: Culicidae) Susceptible and Resistant to Bacillus sphaericus. Journal of Medical Entomology 34, 5–10.CrossRefGoogle ScholarPubMed
Roush, R.T. (1989) Designing resistance management programs: how can you choose? Pesticide Science 26, 423–441.CrossRefGoogle Scholar
Roush, R.T. & Croft, B.A. (1986) Experimental population genetics and ecological studies of pesticide resistance in insects and mites. pp. 257–270in National Research Council, Committee on Strategies for the Management of Pesticide Resistance Pest Populations, Pesticide resistance: strategies and tactics or management. Washington, USA, National Academy Press.Google Scholar
Roush, R.T. & Hoy, M.A. (1981) Laboratory, glasshouse, and field studies of artificially selected carbaryl resistance in Metaseiulus occidentalis. Journal of Economic Entomology 74, 142–147.CrossRefGoogle Scholar
Roush, R.T. & McKenzie, J.A. (1987) Ecological genetics of insecticide and acaricide resistance. Annual Review of Entomology 32, 361–380.CrossRefGoogle ScholarPubMed
Rowland, M. (1991) Behaviour and fitness of gamma HCH/dieldrin resistant and susceptible female Anopheles gambiae and Anopheles stephensi mosquitoes in the absence of insecticide. Medical and Veterinary Entomology 5, 193–206.CrossRefGoogle ScholarPubMed
Thomas, J.G. & Brazzel, J.R. (1961) A compartive study of certain biological phenomena of a resistant and a susceptible strain of the boll weevil, Anthonomus grandis. Journal of Economic Entomology 47, 129–123.Google Scholar
Varzandeh, M., Bruce, W.N. & Decker, G.C. (1954) Resistance to insecticides as a factor influencing the biotic potential of the house fly. Journal of Economic Entomology 54, 417–420.Google Scholar
Wood, R.J. & Bishop, J.A. (1981) Insecticide resistance: populations and evolution. pp. 97–127in Bishop, J.A. & Cook, L.M. (Eds) The Genetic Basis of Man-made Change. New York, Academic Press.Google Scholar