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Body composition, water turnover and energy turnover assessment with labelled water

Published online by Cambridge University Press:  12 June 2007

Klaas R. Westerterp
Klaas R. Westerterp*
Affiliation:
Department of Human Biology, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
*
Corresponding Author: Dr K. Westerterp, fax +31 43 3670976, email k.westerterp@hb.unimaas.NL
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Abstract

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Our understanding of human energy metabolism has benefited greatly from the application of water labelled with 2H and 18O for the measurement of total body water, water turnover and total daily energy expenditure. Applications include validation of techniques for the assessment of dietary intake and physical activity, assessment of water and energy requirement and the assessment of the effect of dietary and physical activity interventions, including its use with endurance athletes competing at the highest level. Critical aspects of the application are isotope dose preparation, sample collection, sample analysis and the calculation procedure. The labelled-water method can easily be applied in normal living conditions, including exercise, and in the clinical setting. However, sample analysis requires a sophisticated laboratory with an isotope-ratio mass spectrometer and a sample preparation system. Examples of insights based on labelled-water studies are: (1) self-reported dietary intakes often underestimate energy requirements; (2) subjects have problems maintaining energy balance when daily energy expenditure exceeds 2.5 times resting energy expenditure. Devices for the assessment of physical activity validated using labelled water allow the study of activity patterns and strategies to influence the activity level of a sedentary society.

Keywords

Energy intakeEnergy requirementEnergy balanceExerciseDoubly-labelled water
Type
Meeting Report
Information
Proceedings of the Nutrition Society , Volume 58 , Issue 4 , November 1999 , pp. 945 - 951
Copyright
The Nutrition Society

References

Begley, IS & Scrimgeour, CM (1997) High-precision d2H and d18O measurement for water and volatile organic compounds by continuous-flow pyrolysis isotope ratio mass spectrometry. Analytical Chemistry 69, 1530–1535.Google Scholar
Black, AE, Coward, WA, Cole, TJ & Prentice, AM (1996) Human energy expenditure in affluent societies: an analysis of 574 doubly-labelled water measurements. European Journal of Clinical Nutrition 50, 72–92.Google ScholarPubMed
Bouten, CVC, Verboeket-van de Venne, WPHG, Westerterp, KR, Verduin, M & Janssen, JD (1996) Physical activity assessment: comparison between movement registration and doubly labeled water. Journal of Applied Physiology 81, 1019–1026.CrossRefGoogle ScholarPubMed
de Bruin, NC, Degenhart, HJ, Gàl, S, Westerterp, KR, Stijnen, T & Visser, HKA (1998) Energy utilization and growth in breast-fed and formula-fed infants, measured prospectively during the first year of life. . American Journal of Clinical Nutrition 67, 885–896.Google Scholar
Forsum, E, Kabir, N, Sadurskis, A & Westerterp, KR (1992) Total energy expenditure of healthy Swedish women during pregnancy and lactation. American Journal of Clinical Nutrition 56, 334–342.CrossRefGoogle ScholarPubMed
Hammond, KA & Diamond, J (1997) Maximal sustained energy budgets in humans and animals. Nature 386, 457–462.CrossRefGoogle ScholarPubMed
Lifson, N & McClintock, R (1966) Theory of use of turnover rates of body water for measuring energy and material balance. Journal of Theoretical Biology 12, 46–74.CrossRefGoogle ScholarPubMed
Prentice, AM (1990) The Doubly-labelled Water Method For Measuring Energy Expenditure, Technical Recommendations For Use In Humans. Section Nutritional and Health Related Environmental Studies, Vienna: International Atomic Energy Agency.Google Scholar
Schoeller, DA (1983) Energy expenditure from doubly labeled water: some fundamental considerations in humans. American Journal of Clinical Nutrition 38, 999–1005.CrossRefGoogle ScholarPubMed
Schoeller, DA & DeLany, JP (1998) Human energy balance: what have we learned from the doubly labeled water method? American Journal of Clinical Nutrition 68, Suppl., 927S–979S.Google Scholar
Schoeller, DA, Leitch, CA & Brown, C (1986) Doubly labeled water method: in vivo oxygen and hydrogen isotope fractionation. American Journal of Physiology 251, R1137–R1143.Google ScholarPubMed
Schoeller, DA & van Santen, E (1982) Measurement of energy expenditure in humans by doubly-labeled water method. Journal of Applied Physiology 53, 955–959.Google Scholar
Speakman, JR (1997) Doubly Labelled Water: Theory and Practice. London: Chapman & Hall.Google Scholar
Speakman, JR & Roberts, SB (1995) Recent advances in the doubly labeled water technique. Obesity Research 3, Suppl. 1, 1–74.CrossRefGoogle Scholar
Scrimgeour, CM, Rollo, MM, Mudambo, SMKT, Handley, LL & Prosser, SJ (1993) A simplified method for deuterium/hydrogen isotope ratio measurements on water samples of biological origin. Biological Mass Spectrometry 22, 383–387.Google Scholar
van den Berg-Emons, HJG, Saris, WHM, de Barbanson, DC, Westerterp, KR, Huson, A & van Baak, MA (1995) Daily physical activity of schoolchildren with spastic diplegia and healthy control subjects. Journal of Pediatrics 127, 578–584.Google Scholar
van Etten, LMLA, Westerterp, KR, Verstappen, FTJ, Boon, BJB & Saris, WHM (1997) Effect of an 18-wk weight-training program on energy expenditure and physical activity. Journal of Applied Physiology 82, 298–304.Google Scholar
van Marken Lichtenbelt, WD, Kester, A, Baarends, EM & Westerterp, KR (1996) Bromide dilution in adults: optimal equilibration time after oral administration. Journal of Applied Physiology 81, 653–656.Google Scholar
van Marken Lichtenbelt, WD, Westerterp, KR & Wouters, L (1994) Deuterium dilution as a method to determine total body water: effect of test protocol and sampling time. British Journal of Nutrition 72, 491–497.Google Scholar
Velthuis-te Wierik, EJM, Westerterp, KR & van den, Berg H (1995) Impact of a moderately energy-restricted diet on energy metabolism and body composition in non-obese men. International Journal of Obesity 19, 318–324.Google Scholar
Westerterp, KR (1998) Alterations in energy balance with exercise. American Journal of Clinical Nutrition 68, 970S–974S.CrossRefGoogle ScholarPubMed
Westerterp, KR & Bouten, CVC (1997) Physical activity assessment: comparison between movement registration and doubly labeled water method. Zeitschrift für Ernährungswissenschaften 36, 263–267.CrossRefGoogle ScholarPubMed
Westerterp, KR, Donkers, J, Fredrix, EWHM & Boekhoudt, P (1995a) Energy intake, physical activity and body weight; a simulation model. British Journal of Nutrition 73, 337–347.CrossRefGoogle ScholarPubMed
Westerterp, KR, Meijer, GAL, Janssen, EME, Saris, WHM & ten, Hoor F (1992) Long term effect of physical activity on energy balance and body composition. British Journal of Nutrition 68, 21–30.CrossRefGoogle ScholarPubMed
Westerterp, KR, Saris, WHM, Van, Es M & ten, Hoor F (1986) Use of the doubly labeled water technique in man during heavy sustained exercise. Journal of Applied Physiology 61, 2162–2167.Google Scholar
Westerterp, KR, Verboeket-van de Venne, WPHG, Bouten, CVC, de Graaf, C, van het, Hof KH & Weststrate, JA (1996) Energy expenditure and physical activity in subjects consuming full- or reduced-fat diets. British Journal of Nutrition 76, 785–795.Google Scholar
Westerterp, KR, Wouters, L & van Marken Lichtenbelt, WD (1995b) The Maastricht protocol for the measurement of body composition and energy expenditure with labeled water. Obesity Research 3, Suppl. 1, 49–57.Google Scholar
Wong, WW, Lee, LS & Klein, PD (1987) Deuterium and 18oxygen measurements on microlitre samples of urine, plasma, saliva and human milk. American Journal of Clinical Nutrition 45, 905–913.CrossRefGoogle Scholar