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Schistosoma mansoni: further studies of the interaction between schistosomula and granulocyte-derived cationic proteins in vitro

Published online by Cambridge University Press:  06 April 2009

Diane J. McLaren ,
C. G. B. Peterson  and
Per Venge
Diane J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
C. G. B. Peterson
Affiliation:
Department of Clinical Chemistry, University of Uppsala, Uppsala, Sweden
Per Venge
Affiliation:
Department of Clinical Chemistry, University of Uppsala, Uppsala, Sweden
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Summary

Purified eosinophil and neutrophil cationic proteins isolated from the lysosomal secretion granules of human leucocytes have been tested for cytotoxic capacity against lung-stage schistosomula of Schistosoma mansoni in vitro. Eosinophil cationic protein (ECP) caused paralysis but not death at high concentration; this effect was reversible and involved no gross pathological manifestations. Eosinophil protein X (EPX) and neutrophil cationic protein (NCP) were highly toxic at concentrations of 10−5 mol/1, and induced paralysis at sublethal concentrations. These two proteins exerted their cidal effect principally against the subtegumental musculature and internal tissues of the parasite rather than against the surface syncytium and tegumental outer membrane. ECP and NCP were shown to bind to lung worms at paralysing concentrations, but this binding occurred independently of the charge of the parasite surface. Of several control proteins tested, only protamine was capable of destroying lung-stage parasites; the manifestations of damage were different, however, from those induced by the granulocyte-derived proteins. Parallel assays demonstrated that EPX also possessed cytotoxic capacity against newly transformed schistosomula, but it was less efficient than ECP in this respect. The data are discussed in relation to potential post-skin mechanisms of challenge attrition in the immunized host.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 3 , June 1984 , pp. 491 - 503
Copyright
Copyright © Cambridge University Press 1984

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References

Clegg, J. A. (1965). In vitro cultivation of Schistosoma mansoni. Experimental Parasitology 16, 133–47.CrossRefGoogle ScholarPubMed
Clegg, J. A. & Smithers, S. R. (1972). The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–98.CrossRefGoogle ScholarPubMed
Dean, D. A. (1983). Schistosoma and related genera: acquired resistance in mice. Experimental Parasitology 55, 1–104.CrossRefGoogle ScholarPubMed
Durack, D. T., Ackerman, S. J., Loegering, D. A. & Gleich, G. J. (1981). Purification of human eosinophil-derived neurotoxin. Proceedings of the National Academy of Sciences 78, 5165–9.CrossRefGoogle ScholarPubMed
Durack, D. T., Sumi, S. M. & Klebanoff, S. J. (1979). Neurotoxicity of human eosinophils. Proceedings of the National Academy of Sciences 76, 1443–7.CrossRefGoogle ScholarPubMed
Fresdens, K., Dahl, R. & Venge, P. (1982). The Gordon Phenomenon induced by the eosinophil cationic protein and eosinophil protein X. Journal of Allergy and Clinical Immunology 70, 361–6.CrossRefGoogle Scholar
Gleich, G. J., Loegering, D. A., Frigas, E., Wassom, D. L., Solley, G. O. & Mann, K. G. (1980). The major basic protein of the eosinophil granule: physicochemical properties, localization and function. In The Eosinophil in Health and Disease (ed. Mahmoud, A. A. F. and Austen, K. F.), p. 79. New York: Grune Stratton.Google Scholar
Gordon, M. H. (1933). Remarks on Hodgkin's Disease. A pathogenic agent in the glands, and its application in diagnosis. British Medical Journal 1, 641–4.CrossRefGoogle ScholarPubMed
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 13–25.CrossRefGoogle ScholarPubMed
Incani, R. N. & McLaren, D. J. (1981). Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: studies of the kinetics of complement and/or antibody-dependent adherence and killing. Parasite Immunology 3, 107–26.CrossRefGoogle ScholarPubMed
Lichtenberg, F. von & Byram, J. E. (1980). Pulmonary cell reactions in natural and acquired host resistance to Schistosoma mansoni. American Journal for Tropical Medicine and Hygiene 29, 1286–300.CrossRefGoogle Scholar
Mangold, B. L. & Knopf, P. M. (1981). Host protective humoral immune responses to Schistosoma mansoni infections in the rat. Kinetics of hyperimmune serum-dependent sensitivity and elimination of schistosomes in a passive transfer system. Parasitology 83, 559–74.CrossRefGoogle Scholar
McLaren, D. J. (1974). The anterior glands of adult Necator americanus (Nematoda: Strongyloidea). 1. Ultrastructural studies. International Journal for Parasitology 4, 25–37.CrossRefGoogle ScholarPubMed
McLaren, D. J. (1980). Schistosoma mansoni: the parasite surface in relation to host immunity. In Tropical Medicine Research Studies vol. 1, (ed. Brown, K. N.), pp. 1–229. Chichester: Research Studies Press/John Wiley.Google Scholar
McLaren, D. J., Glegg, J. A. & Smithers, S. R. (1975). Acquisition of host antigens by young Schistosoma mansoni in mice: correlation with failure to bind antibody in vitro. Parasitology 70, 67–75.CrossRefGoogle ScholarPubMed
McLaren, D. J., McKean, J. R., Olsson, I., Venge, P. & Kay, A. B. (1981). Morphological studies on the killing of schistosomula of Schistosoma mansoni by human eosinophil and neutrophil cationic proteins in vitro. Parasite Immunology 3, 359–73.CrossRefGoogle ScholarPubMed
McLaren, D. J. & Ramalho-Pinto, F. J. (1979). Eosinophil-mediated killing of schistosomula of Schistosoma mansoni in vitro: synergistic effect of antibody and complement. Journal of Immunology 123, 1431–8.CrossRefGoogle ScholarPubMed
McLaren, D. J. & Smithers, S. R. (1982). Immunity to schistosomiasis: in vitro versus in vivo models. In Animal Models in Parasitology (ed. Owen, D. G.), pp. 213–27. London: Macmillan Press.CrossRefGoogle Scholar
McLaren, D. J. & Terry, R. J. (1982). The protective role of acquired host antigens during schistosome maturation. Parasite Immunology 4, 129–48.CrossRefGoogle ScholarPubMed
Olsson, I. & Venge, P. (1974). Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukemic myeloid cells. Blood 44, 235–46.CrossRefGoogle ScholarPubMed
Olsson, I., Venge, P., Spitznagel, J. K. & Lehrer, R. I. (1977). Arginine-rich cationic proteins of human eosinophil granules. Comparison of the constituents of eosinophilic and neutrophilic leucocytes. Laboratory Investigation 36, 493–500.Google Scholar
Peterson, C. G. B. & Venge, P. (1983). Purification and characterization of a new cationic protein – eosinophil protein X (EPX) –from granules of human eosinophils. Immunology 50, 19–26.Google ScholarPubMed
Pincus, S. H., Butterworth, A. E., David, J. R., Robbins, M. & Vadas, M. A. (1981). Antibody-dependent eosinophil mediated damage to schistosomula of Schistosoma mansoni: lack of requirement for oxidative metabolism. Journal of Immunology 126, 1794–9.CrossRefGoogle ScholarPubMed
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: a defined system for the stepwise transformation of the cercaria to schistosomule in vitro. Experimental Parasitology 36, 360–72.CrossRefGoogle ScholarPubMed
Ramalho-Pinto, F. J., McLaren, D. J. & Smithers, S. R. (1978). Complement-mediated killing of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. Journal of Experimental Medicine 147, 147–56.CrossRefGoogle ScholarPubMed
Rumjanek, F. D. (1980). Biochemical adaptation during Schistosoma mansoni infection. In The Host Invader Interplay (ed. Van den Bossche, H.), pp. 139–42. Amsterdam: Elsevier/North Holland Biomedical Press.Google Scholar
Rumjanek, F. D. & McLaren, D. J. (1981). Schistosoma mansoni: modulation of schistosomular lipid composition by serum. Molecular and Biochemical Parasitology 3, 239–52.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1977). The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni. Parasitology 74, 61–71.CrossRefGoogle ScholarPubMed