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Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community

Published online by Cambridge University Press:  03 March 2004

R. J. TRAUB ,
P. T. MONIS ,
I. ROBERTSON ,
P. IRWIN ,
N. MENCKE  and
R. C. A. THOMPSON
R. J. TRAUB
Affiliation:
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the Western Australian Biomedical Research Institute, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150
P. T. MONIS
Affiliation:
Australian Water Quality Centre, Bolivar SA 5110, Australia
I. ROBERTSON
Affiliation:
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the Western Australian Biomedical Research Institute, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150
P. IRWIN
Affiliation:
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the Western Australian Biomedical Research Institute, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150
N. MENCKE
Affiliation:
Bayer AG, BG-Animal Health, D-51368 Leverkusen, Germany
R. C. A. THOMPSON
Affiliation:
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the Western Australian Biomedical Research Institute, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150
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Abstract

Giardia duodenalis isolates recovered from humans and dogs living in the same locality in a remote tea-growing community of northeast India were characterized at 3 different loci; the SSU-rDNA, elongation factor 1-alpha (ef1-α) and triose phosphate isomerase (tpi) gene. Phylogenetic analysis of the SSU-rDNA and ef1-α genes provided poor genetic resolution of the isolates within various assemblages, stressing the importance of using multiple loci when inferring genotypes to Giardia. Analysis of the tpi gene provided better genetic resolution and placed canine Giardia isolates within the genetic groupings of human isolates (Assemblages A and B). Further evidence for zoonotic transmission was supported by epidemiological data showing a highly significant association between the prevalence of Giardia in humans and presence of a Giardia-positive dog in the same household (odds ratio 3·01, 95% CI, 1·11, 8·39, P=0·0000).

Keywords

GiardiadogszoonosesIndia
Type
Research Article
Information
Parasitology , Volume 128 , Issue 3 , March 2004 , pp. 253 - 262
Copyright
2004 Cambridge University Press

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