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Application of physico-chemical typing methods for the epidemiological analysis of Salmonella enteritidis strains of phage type 25/17

Published online by Cambridge University Press:  19 October 2009

G. Seltmann ,
W. Voigt  and
W. Beer
G. Seltmann
Affiliation:
Robert-Koch-Institut des Bundesgesundheitsamtes, Bereich Wernigerode, Postfach, D-38843 Wernigerode, Germany
W. Voigt
Affiliation:
Robert-Koch-Institut des Bundesgesundheitsamtes, Bereich Wernigerode, Postfach, D-38843 Wernigerode, Germany
W. Beer
Affiliation:
Robert-Koch-Institut des Bundesgesundheitsamtes, Bereich Wernigerode, Postfach, D-38843 Wernigerode, Germany
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Summary

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Eighty-nine Salmonella enteritidis phage type 25/17 strains isolated from a localized outbreak in the German state Nordrhein-Westfalen (outbreak NWI) could not be further differentiated by biochemotyping and plasmid pattern analysis. They were submitted to a complex typing system consisting of modern physico-chemical analytical procedures. In lipopolysaccharide pattern analysis the strains proved to be homogeneous. In multilocus enzyme electrophoresis, outer membrane and whole cell protein pattern (WCPP) analysis, and Fourier transform infrared (FT–IR) spectroscopy (increasing extent of differentiation in the given order) strains deviating from each basal pattern were found. The extent of correspondence in these deviations was satisfactory.

Forty-six strains of the same sero- and phage type, however, obtained from different outbreaks, were additionally typed. The results obtained with them indicate that the data of the first group were not restricted to strains from outbreak NWI, but of general validity.

It was found that both WCPP and FT–IR represent valuable methods for the sub-grouping of bacteria.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 3 , December 1994 , pp. 411 - 424
Copyright
Copyright © Cambridge University Press 1994

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