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Detection of Aeromonas caviae in the common housefly Musca domestica by culture and polymerase chain reaction

Published online by Cambridge University Press:  05 February 2002

D. NAYDUCH ,
A. HONKO ,
G. P. NOBLET  and
F. STUTZENBERGER
D. NAYDUCH
Affiliation:
Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA
A. HONKO
Affiliation:
Department of Microbiology and Molecular Medicine, Clemson University, Clemson, South Carolina, USA
G. P. NOBLET
Affiliation:
Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA
F. STUTZENBERGER
Affiliation:
Department of Microbiology and Molecular Medicine, Clemson University, Clemson, South Carolina, USA
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Abstract

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Aeromonas caviae has been implicated in diarrhoeal disease of livestock and humans. The potential role of houseflies in the epidemiology of this pathogen was investigated by examining the prevalence of A. caviae in houseflies collected from two South Carolina farms and one restaurant. Isolation was accomplished by culture of flies in alkaline peptone water followed by identification with Aeromonas-specific PCR using novel primers (APW–PCR). All isolates cultured from houseflies were identified as A. caviae by biochemical characteristics and direct sequencing ∼ 800 bp of the 16S rRNA gene. Aeromonas caviae was detected in 78% (272/349) dairy farm flies, 55% (54/99) pig farm flies and 39% (77/200) restaurant flies. Faeces from cows and pigs at the farms also were positive for A. caviae (58% and 100%, respectively). The APW–PCR method provided a rapid, convenient way to identify A. caviae from faeces and houseflies that contained hundreds of bacterial species.

Type
Research Article
Information
Epidemiology & Infection , Volume 127 , Issue 3 , December 2001 , pp. 561 - 566
Copyright
2001 Cambridge University Press