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Amacrine and bipolar inputs to midget and parasol ganglion cells in marmoset retina

Published online by Cambridge University Press:  08 May 2012

CARLA J. ABBOTT ,
KUMIKO A. PERCIVAL ,
PAUL R. MARTIN  and
ULRIKE GRÜNERT
CARLA J. ABBOTT
Affiliation:
Department of Ophthalmology and Save Sight Institute, The University of Sydney, Sydney, New South Wales, Australia Australian Research Council Centre of Excellence in Vision Science, The University of Sydney, Sydney, New South Wales, Australia
KUMIKO A. PERCIVAL
Affiliation:
Department of Ophthalmology and Save Sight Institute, The University of Sydney, Sydney, New South Wales, Australia Australian Research Council Centre of Excellence in Vision Science, The University of Sydney, Sydney, New South Wales, Australia
PAUL R. MARTIN
Affiliation:
Department of Ophthalmology and Save Sight Institute, The University of Sydney, Sydney, New South Wales, Australia Australian Research Council Centre of Excellence in Vision Science, The University of Sydney, Sydney, New South Wales, Australia
ULRIKE GRÜNERT*
Affiliation:
Department of Ophthalmology and Save Sight Institute, The University of Sydney, Sydney, New South Wales, Australia Australian Research Council Centre of Excellence in Vision Science, The University of Sydney, Sydney, New South Wales, Australia
*
*Address correspondence and reprint requests to: Ulrike Grünert, Save Sight Institute, Sydney Eye Hospital Campus, GPO Box 4337, Sydney, NSW 2001, Australia, E-mail: ugrunert@sydney.edu.au
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Abstract

Retinal ganglion cells receive excitatory synapses from bipolar cells and inhibitory synapses from amacrine cells. Previous studies in primate suggest that the strength of inhibitory amacrine input is greater to cells in peripheral retina than to foveal (central) cells. A comprehensive study of a large number of ganglion cells at different eccentricities, however, is still lacking. Here, we compared the amacrine and bipolar input to midget and parasol ganglion cells in central and peripheral retina of marmosets (Callithrix jacchus). Ganglion cells were labeled by retrograde filling from the lateral geniculate nucleus or by intracellular injection. Presumed amacrine input was identified with antibodies against gephyrin; presumed bipolar input was identified with antibodies against the GluR4 subunit of the AMPA receptor. In vertical sections, about 40% of gephyrin immunoreactive (IR) puncta were colocalized with GABAA receptor subunits, whereas immunoreactivity for gephyrin and GluR4 was found at distinct sets of puncta. The density of gephyrin IR puncta associated with ganglion cell dendrites was comparable for midget and parasol cells at all eccentricities studied (up to 2 mm or about 16 degrees of visual angle for midget cells and up to 10 mm or >80 degrees of visual angle for parasol cells). In central retina, the densities of gephyrin IR and GluR4 IR puncta associated with the dendrites of midget and parasol cells are comparable, but the average density of GluR4 IR puncta decreased slightly in peripheral parasol cells. These anatomical results indicate that the ratio of amacrine to bipolar input does not account for the distinct functional properties of parasol and midget cells or for functional differences between cells of the same type in central and peripheral retina.

Keywords

Primate retinaInhibitory synapsesCallithrix jacchusExcitatory synapses
Type
Research Articles
Information
Visual Neuroscience , Volume 29 , Issue 3 , May 2012 , pp. 157 - 168
Copyright
Copyright © Cambridge University Press 2012

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