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Intracytoplasmic sperm injection in dysmorphic human oocytes

Published online by Cambridge University Press:  26 September 2008

Mina Alikani ,
Gianpiero Palermo ,
Alexis Adler ,
Massimo Bertoil ,
Marlena Blake  and
Jacques Cohen
Mina Alikani*
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Gianpiero Palermo
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Alexis Adler
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Massimo Bertoil
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Marlena Blake
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Jacques Cohen
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
*
Mina Alikani, The Institute for Reproductive Medicine and Science, Saint Barbabas Medical Center, 101 Old Short Hills Road, Suite 501, West Orange, NJ 07052, USA. Telephone: 201–234–6237. Fax: 201–234–6235.
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Summary

Fertilisation and development of dysmorphic human oocytes recovered from hyperstimulated ovaries have been evaluated following intracytoplasmic sperm injection (ICSI) for treatment of male infertility. A total of 2968 oocytes at metaphase II of meiosis were injected, of which 806 (27.2%) were dysmorphic at the light microscopic level. Cytoplasmic abnormalities included granularity, areas of necrosis, organelle clustering, vacuoles, and accumulating saccules of smooth endoplasmic reticulum. Anomalies of the first polar body and zona pellucida, as well as non-spherical shapes of oocytes, were also noted. Contrary to previous findings linking some dysmorphisms to non-assisted fertilisation failure, in this study no single abnormality led to a reduction in the fertilisation rate, nor was fertilisation compromised in oocytes with multiple abnormalities. The incidence of normal fertilisation (two pronuclei and two polar bodies) was 69% in both the dysmorphic and non-dysmorphic oocytes. While overall pregnancy and implantation results were not altered in the group of patients (n = 242) in whom at least one dysmorphic oocyte was injected, exclusive replacement of embryos which originated from dysmorphic oocytes led to a higher incidence of early pregnancy loss. It is concluded that aberrations in the morphology of human oocytes – most probably a product of controlled ovarian stimulation – are of little or no consequence to fertilisation or early cleavage after ICSI. It is possible, however, that these embryos have a reduced potential for implantation and further development.

Keywords

DysmorphicICSIImplantationOocytePregnancy loss
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 283 - 288
Copyright
Copyright © Cambridge University Press 1995

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