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The role of porcine reproductive and respiratory syndrome (PRRS) virus structural and non-structural proteins in virus pathogenesis

Published online by Cambridge University Press:  14 April 2010

Nedzad Music  and
Carl A. Gagnon
Nedzad Music
Affiliation:
Service de diagnostic, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada Groupe de recherche sur les maladies infectieuses du porc (GREMIP), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada
Carl A. Gagnon*
Affiliation:
Service de diagnostic, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada Groupe de recherche sur les maladies infectieuses du porc (GREMIP), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada Centre de recherche en infectiologie porcine (CRIP), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada
*
*Corresponding author. E-mail: carl.a.gagnon@umontreal.ca
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Abstract

Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease affecting the swine industry worldwide. The etiological agent, PRRS virus (PRRSV), possesses a RNA viral genome with nine open reading frames (ORFs). The ORF1a and ORF1b replicase-associated genes encode the polyproteins pp1a and pp1ab, respectively. The pp1a is processed in nine non-structural proteins (nsps): nsp1α, nsp1β, and nsp2 to nsp8. Proteolytic cleavage of pp1ab generates products nsp9 to nsp12. The proteolytic pp1a cleavage products process and cleave pp1a and pp1ab into nsp products. The nsp9 to nsp12 are involved in virus genome transcription and replication. The 3′ end of the viral genome encodes four minor and three major structural proteins. The GP2a, GP3 and GP4 (encoded by ORF2a, 3 and 4), are glycosylated membrane associated minor structural proteins. The fourth minor structural protein, the E protein (encoded by ORF2b), is an unglycosylated membrane associated protein. The viral envelope contains two major structural proteins: a glycosylated major envelope protein GP5 (encoded by ORF5) and an unglycosylated membrane M protein (encoded by ORF6). The third major structural protein is the nucleocapsid N protein (encoded by ORF7). All PRRSV non-structural and structural proteins are essential for virus replication, and PRRSV infectivity is relatively intolerant to subtle changes within the structural proteins. PRRSV virulence is multigenic and resides in both the non-structural and structural viral proteins. This review discusses the molecular characteristics, biological and immunological functions of the PRRSV structural and nsps and their involvement in the virus pathogenesis.

Keywords

PRRSviral pathogenesisstructural proteinsnon-structural proteinsvirulencemolecular characteristicsimmunological functions
Type
Review Article
Information
Animal Health Research Reviews , Volume 11 , Issue 2 , December 2010 , pp. 135 - 163
Copyright
Copyright © Cambridge University Press 2010

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