Abstract
A consensus primer PCR approach was used to (i) investigate the presence of herpesviruses in wild and zoo equids (zebra, wild ass, tapir) and to (ii) study the genetic relationship of the herpesvirus of pigeons (columbid herpesvirus 1) to other herpesvirus species. The PCR assay, based on degenerate primers targeting highly conserved regions of the DNA polymerase gene of herpesviruses, was modified by using a mixture of degenerate and deoxyinosine-substituted primers. The applicability of the modification was validated by amplification of published DNA polymerase genes of 16 herpesvirus species and of the previously uncharacterized DNA polymerase genes of equine herpesvirus 3 (EHV-3) and equine herpesvirus 5 (EHV-5). The modified assay was then used for partial amplification of the polymerase of columbid herpesvirus 1 which is presently classified as a β-herpesvirus based on biological criteria. Sequence analysis of amplicons obtained from four different viral strains revealed a close relationship of columbid herpesvirus 1 to members of the subfamily Alphaherpesvirinae, especially to Marek’s disease herpesvirus. This was confirmed by characterization of additional 1.6 kb of the columbid herpesvirus 1 polymerase. Consensus PCR analysis of blood samples from zebras, a wild ass and a tapir revealed amplicons showing high percentages (>50%) of sequence identity to DNA polymerases of γ-herpesviruses. In particular, the zebra and the wild ass sequence were closely related to each other and to the polymerases of the equine γ-herpesviruses EHV-2 and EHV-5 with sequence identities of >80%. This is a first indication that novel γ-herpesviruses are present in wild and zoo equids.
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References
Borchers K. and FroÈlich K., J Wildlife Dis 33, 812-817, 1997
Messana M., Kuesters J., and Grund C., Avian Pathology 26, 859-864, 1997
Ziemann K., Mettenleiter T.C., and Fuchs W., J Virol 72, 847-852, 1998
Buckmaster A.E., Scott S.D., Sanderson M.J., Boursnell M.E.G., Ross N.L.J., and Binns M.M., J Gen Virol 69, 2033-2042, 1988
Kaleta E.F., Avian Pathology 26, 305-316, 1997
VanDevanter D.R., Warrener P., Bennett L., Schultz E.R., Coulter S., Garber R.L., and Rose T.M., J Clin Microbiol 34, 1666-1671, 1996
Ehlers B., Buhk H.-J., and Ludwig H., J Gen Virol 66, 55-68, 1985
Kebelmann-Betzing C., Seeger K., Dragon S., Schmitt G., MoÈricke A., Schild T.A., Henze G., and Beyermann B., BioTechniques 24, 154-158, 1998
Rossolini G.M., Cresti S., Ingianni A., Cattani P., Riccio M.L., and Satta S., Mol Cell Probes 8, 91-98, 1994
Knoth K., Roberds S., Poteet C., and Tamkun M., Nucl Acids Res 16, 10932, 1988
Cassol S., Salas T., Lapointe N., Arella M., Rudnik J., and O'Shaughnessy M., Mol Cell Probes 5, 157-160, 1991
McGeoch D.J., Cook S., Dolan A., Jamieson F.E., and Telford E.A.R., J Mol Biol 247, 443-458, 1995
Rovnak J., Quackenbush S.L., Reyes R.A., Baines J.D., Parrish, C.R., and Casey J.W., J Virol, 72, 4237-4242, 1998
Cornwell H.J.C. and Wright N.G., J Comp Path 80, 221-226, 1970
Sallmann M., (Diss. vet. med.), University of Giessen, 1991
Knopf C.W., Virus Genes 16, 47-58, 1998
Desrosiers R.C., Sasseville V.G., Czajak S.C., Zhang X., Mans®eld K.G., Kaur A., Johnson P., Lackner A.A., and Jung J.U., J Virol 71, 9764-9769, 1998
Borchers K., Wol®nger U., Goltz M., Broll H., and Ludwig H., Arch Virol, 142, 917-928, 1997
Browning C.F. and Studdert M.J., Vet Bull 58, 775-790, 1988
Telford E.A.R., Studdert M.J., Agius C.T., Watson M.S., Aird H.C., and Davison A.J., Virology 195, 492-499, 1993
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Ehlers, B., Borchers, K., Grund, C. et al. Detection of New DNA Polymerase Genes of Known and Potentially Novel Herpesviruses by PCR with Degenerate and Deoxyinosine-Substituted Primers. Virus Genes 18, 211–220 (1999). https://doi.org/10.1023/A:1008064118057
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DOI: https://doi.org/10.1023/A:1008064118057