Abstract
Catalytic activities of lingual lipase were investigated by enzyme histochemistry in post-mortem tongues from male rats. Sections of fresh-frozen or formalin-calcium fixed tissue were incubated with naphthol-AS-nonanoate and α-naphthyl acetate substrate mixtures. The effects of pH level, sodium taurocholate activator and E600 inhibitor were also examined. The use of cryostat sections of tissues fixed in formalin-calcium and of nonanoate substrate within the range of pH 4.4–6.4, were optimal for localizing maximum reaction product, captured by Fast Blue BB, in acini and demilunes of the posterior deep and superficial lingual glands respectively. The reaction product corresponded with the distribution of secretory granules and failed to develop when taurocholate was omitted from the incubation medium. Similarly localized E600-resistant reaction product occurred with the acetate substrate and hexazotized New Fuchsin at pH 7.4, in the absence of taurocholate. Lipase and conventional esterase activities appear to be superimposed in posterior lingual glands of rat. The ability of their acini and demilunes to hydrolyse nonanoate substrate at an acidic pH optimum, when activated by sodium taurocholate, seems attributable to lipase destined for secretion into saliva – hence convenient for routine histochemical identification of the enzyme.
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References
Abe M, Kramer SP, Seligman AM (1964) The histochemical demonstration of pancreatic-like lipase and comparison with the distribution of esterase. J Histochem Cytochem 12: 364–383.
Barrowman JA, Darnton SJ (1970) The lipase of rat gastric mucosa. A histochemical demonstration of the enzymatic activity against a medium chain triglyceride. Gastroenterology 59: 13–21.
Darnton SJ, Barrowman JA (1969) A specific histochemical method for the demonstration of the activity of pancreatic lipase. Histochem J 1: 551–557.
Davis BJ (1959) Histochemical demonstration of erythrocyte esterases. Proc Soc Exp Biol Med 101: 90–93.
DeNigris SJ, Hamosh M, Kasbekar DK, Lee TC, Hamosh P (1988) Lingual and gastric lipases: Species differences in the origin of prepancreatic digestive lipases and in the localization of gastric lipase. Biochim Biophys Acta 959: 38–45.
Field RB, Scow RO (1983) Purification and characterization of lingual lipase. J Biol Chem 258: 14563–14569.
Field RB, Spielman AI, Hand AR (1989) Purification of lingual amylase from serous glands of rat tongue and characterization of rat lingual amylase and lingual lipase. J Dent Res 68: 139–145.
Garrett JR, Winston DC, Proctor GB, Schulte BA (1992) Na,K-ATPase in resting and stimulated submandibular salivary glands in cats, studied by means of ouabain sensitive, K+-dependent p-nitrophenyl phosphatase activity. Arch Oral Biol 37: 711–716.
Gomori G (1945) The microtechnical demonstration of sites of lipase activity. Proc Soc Exp Biol Med 58: 362–364.
Hamosh M (1990) Lingual and gastric lipases. Nutrition 6: 421–428.
Hamosh M, Burns WA (1977) Lipolytic activity of human lingual glands (Ebner). Lab Invest 37: 603–608.
Hamosh M, Scow RO (1973) Lingual lipase and its role in the digestion of dietary lipid. J Clin Invest 52: 88–95.
Hand AR (1970) The fine structure of von Ebner's gland of the rat. J Cell Biol 44: 340–353.
Hand AR, Pathmanathan D, Field RB (1999) Morphological features of the minor salivary glands. Arch Oral Biol 44: S3–S10.
Knospe C, Plendl J (1997) Histochemical demonstration of lipase activity in the gastric mucosa of cat. Anat Histol Embryol 26: 303–304.
Lake BD (1972) Is the histochemical demonstration of lipase activity possible? Histochem J 4: 71–73.
Mahadevan S, Tappel AL (1968) Lysosomal lipases of rat liver and kidney. J Biol Chem 243: 2849–2854.
Mira E (1965) Cytochemical localization of oxidative and hydrolytic enzymes in von Ebner's glands. Acta Otolaryngol (Stock) 59: 88–96.
Moreau H, Bernadac A, Gargouri Y, Benkouka F, Laugier R, Verger R (1989) Immunocytolocalization of human gastric lipase in chief cells of the fundic mucosa. Histochemistry 91: 419–423.
Moreau H, Gargouri Y, Lecat D, Junien JL, Verger R (1988) Screening of preduodenal lipases in several mammals. Biochim Biophys Acta 959: 247–252.
Nagato T, Ren X-Z, Toh H, Tandler B (1997) Ultrastructure of Weber's salivary glands of the root of tongue of rat. Anat Rec 249: 435–440.
Pearse AGE (1960) Histochemistry: Theoretical and Applied. 2nd edn. London: Churchill. pp. 363–383, 456–490.
Roberts IM, Jaffe R (1986) Lingual lipase: Immunocytochemical localization in the rat von Ebner gland. Gastroenterology 90: 1170–1175.
Roberts IM, Nochomovitz LE, Jaffe R, Hanel SI, Rojas M, Agostini Jr RA (1987) Immunocytochemical localization of lingual lipase in serous cells of the developing rat tongue. Lipids 22: 764–766.
Schaffner T, Elner V, Bauer M, Wissler RW (1978) Acid lipase: A histochemical and biochemical study using Triton X100-naphthyl palmitate micelles. J Histochem Cytochem 26: 696–712.
Seligman AM (1972) Concerning overlapping activities of related hydrolases and their inconvenience to scientific study. Histochem J 4: 561–563.
Tandler B, Riva A (1986) Salivary glands. In: Mjör IA, Fejerskov O, eds. Human Oral Embryology and Histology. Copenhagen: Munksgaard, pp. 243–284.
Triantafyllou A, Fletcher D, Scott JS (2001) Histochemical phenotypes of von Ebner's gland of ferret and their functional implications. Histochem J 31: 173–181.
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Triantafyllou, A., Fletcher, D. & Scott, J. Lipolytic and Esterolytic Activities in Posterior Lingual Glands of Rat: A Histochemical Study. Histochem J 34, 529–533 (2003). https://doi.org/10.1023/A:1026037801958
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DOI: https://doi.org/10.1023/A:1026037801958