Abstract
ALTHOUGH many unsuccessful attempts have been made to transform normal human cells to the neoplastic state1–4, the putative transformations reported have been primarily with cell lines5,6 derived from xeroderma pigmentosum biopsies or osteosarcoma tumour tissues7–9. Recently, cells from a skin biopsy taken from the lip of an adult female were reported to be transformed by 4-nitroquinoline oxide and N-methyl-N1-nitronitrosoguanidine10 (MNNG). We now report that primary or low passage cells in vitro from various human foreskin cell populations can be neoplastically transformed in vitro by six different chemical carcinogens. A close correlation between growth in soft agar and tumour formation in nude mice was obtained.
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MILO, G., DIPAOLO, J. Neoplastic transformation of human diploid cells in vitro after chemical carcinogen treatment. Nature 275, 130–132 (1978). https://doi.org/10.1038/275130a0
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DOI: https://doi.org/10.1038/275130a0
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