Abstract
Infections of mammalian malaria parasites start when sporozoites from an infected anopheline mosquito are injected into the bloodstream of the host. The sporozoites enter the hepatocytes and become transformed into exoerythrocytic schizonts. Since the discovery of the primate parasite Plasmodium cynomolgi in monkey hepatocytes1 and the rodent parasite Plasmodium berghei in hamster hepatocytes2, the ultrastructure of these stages has been extensively studied both in primate3,4 and rodent plasmodia5–10. These observations relate only to the development of the exoerythrocytic schizont 25 h8 after sporozoite injection until the final maturation (of P. berghei) 50 h post-inoculation. Recently, we have studied the route of entry of sporozoites across the cellular lining of liver sinusoids and invasion of the liver parenchymal cells11–13 by using transmission electron microscopy. The results of these studies in combination with other physiological experiments14–17 strongly suggested that the sporozoite was initially harboured by the Kupffer cell, from which the parasite escaped into the neighbouring hepatocyte. The migration of sporozoites from liver sinusoids to hepatocytes can be achieved within a few minutes18. We present here the first ultrastructural observations on the natural transformation of intrahepatocytic sporozoites into exoerythrocytic forms in vivo, using the rodent malaria parasite P. berghei in a laboratory host, the Brown Norway rat. These observations complete the search for the final link in the life cycle of malaria parasites.
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References
Shortt, H. E. & Garnham, P. C. C. Nature 161, 126 (1948).
Yoeli, M. & Most, H. Nature 205, 715–716 (1965).
Sodeman, T., Schnitzer, B., Durkee, T. & Contacos, P. Science 170, 340–341 (1970).
Boulard, Y., Druilhe, P., Miltgen, F., Landau, I. & Rinjard, J. Protistologica 18, 253–258 (1982).
Garnham, P. C. C., Bird, R. G., Baker, J. R. & Killick-Kendrick, R. Trans. R. Soc. trop. Med. Hyg. 63, 328–332 (1969).
Desser, S. S., Weller, I. & Yoeli, M. Can J. Zool. 50, 821–825 (1972).
Seureau, C., Szollosi, A., Boulard, Y., Landau, I. & Peters, W. Prostistologica 16, 419–426 (1980).
Meis, J. F. G. M., Verhave, J. P., Jap, P. H. K., Hess, F. & Meuwissen, J. H. E. T. Parasitology 82, 195–204 (1981).
Jap, P. H. K., Meis, J. F. G. M., Verhave, J. P. & Meuwissen, J. H. E. T. Parasitology 85, 263–269 (1982).
Meis, J. F. G. M., Jap, P. H. K., Verhave, J. P. & Meuwissen, J. H. E. T. J. Protozool. 30, 111–114 (1983).
Meis, J. F. G. M., Verhave, J. P., Jap, P. H. K. & Meuwissen, J. H. E. T. Z. Parasitenkd 67, 345–348 (1982).
Meis, J. F. G. M., Verhave, J. P., Jap, P. H. K. & Meuwissen, J. H. E. T. Sinusoidal Liver Cells (eds Knook, D. L. & Wisse, E.) 429–436 (Elsevier, Amsterdam, 1982).
Meis, J. F. G. M., Verhave, J. P., Jap, P. H. K. & Meuwissen, J. H. E. T. Parasitology 86, 231–242 (1983).
Verhave, J. P., Meuwissen, J. H. E. T. & Golenser, J. Int. J. nucl. Med. Biol. 7, 149–156 (1980).
Sinden, R. E. & Smith, J. E. Acta trop. 39, 11–27 (1982).
Danforth, H. D., Moon, R. J., Jensen, J. B., Vrable, R.G. & Beaudoin, R. L. Acta trop. 39, 5–10 (1982).
Smith, J. E. & Sinden, R. E. in Sinusoidal Liver Cells (eds Knook, D. L. & Wisse, E.) 437–444 (Elsevier, Amsterdam, 1982).
Shin, S. C. J., Vanderberg, J. P. & Terzakis, J. A. J. Protozool. 29, 448–454 (1982).
Vermeulen, A. N., Van Munster, J. C. & Meuwissen, J. H. E. T. Expl Parasit. 53, 199–208 (1982).
Danforth, H. D., Aikawa, M., Cochrane, A. H. & Nussenzweig, R. S. J. Protozool. 27, 193–202 (1980).
Holmberg, S., Schulman, S. & Vanderberg, J. P. J. Parasit. 67, 893–897 (1981).
Jap, P. H. K. thesis, Univ. Nijmegen (1971).
Ladda, R. L. Milit. Med. 134, 825–864 (1969).
Canning, E. U. & Sinden, R. E. Parasitology 67, 29–40 (1973).
Bannister, L. H., Butcher, G. A. & Mitchell, G. H. Bull. Wld Hlth Org. 55, 163–169 (1977).
Sinden, R. E. in Rodent Malaria (eds Killick-Kendrick, R. & Peters, W.) 85–168 (Academic, London, 1978).
Krotoski, W. A. et al. Am. J. trop. Med. Hyg. 31, 24–35 (1982).
Krotoski, W. A. et al. Am. J. trop. Med. Hyg. 32, 211–225 (1982).
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Meis, J., Verhave, J., Jap, P. et al. Malaria parasites—discovery of the early liver form. Nature 302, 424–426 (1983). https://doi.org/10.1038/302424a0
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DOI: https://doi.org/10.1038/302424a0
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