Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Comparative morphological studies on the carcinogenic effect of 7,12-dimethylbenz(A)anthracene (DMBA) in normal or intrasplenic ovarian tissue of C3H mice

Abstract

A single intravenous injection of 100 mg/kg body weight (b.w.) of 7, 12 dimethylbenz(a)anthracene (DMBA) induces a high percentage of ovarian granulosa cell tumours in C3H mice. After implantation of ovarian tissue into the spleen of gonadectomized female C3H mice similar tumours were found, resulting from an over-stimulation by pituitary gonadotrophins. In the present study the tumour development in intrasplenic ovarian tissue was observed after an additional single intravenous application of 100 mg/kg b.w. DMBA. It was found that the induction of granulosa cell tumours did not seem to be affected by the carcinogen injection whether 12 weeks before or 12 weeks after ovarian tissue was implanted into the spleen. The morphology of these neoplasms corresponds to the DMBA induced granulosa cell tumours in orthotopic ovaries. A direct carcinogenic effect of DMBA on ovarian cells in mice could not be demonstrated but there are indications that the additional DMBA application accelerated the destruction of the oocytes, which might result in a more rapid intrasplenic tumour induction.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Authors

Additional information

Supported by Deutsche Forschungsgemeinschaft.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hilfrich, J. Comparative morphological studies on the carcinogenic effect of 7,12-dimethylbenz(A)anthracene (DMBA) in normal or intrasplenic ovarian tissue of C3H mice. Br J Cancer 32, 588–595 (1975). https://doi.org/10.1038/bjc.1975.265

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/bjc.1975.265

This article is cited by

Search

Quick links