Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Post-Transplants Events

No impact of NOD2/CARD15 on outcome after SCT

Bone Marrow Transplantation volume 41, pages 961–964 (2008)Cite this article

Abstract

Recent studies have pointed towards an association between certain single nucleotide polymorphisms (SNPs) in the NOD2/CARD15 gene, and negative outcome of Allo-SCT. In this study, 198 patients and their corresponding donors were analyzed retrospectively for the occurrence of NOD2/CARD15 mutations to evaluate the impact on clinical results after Allo-SCT. In all, 7.6% of the patients and 11% of the donors were heterozygous for one of three SNPs 8, 12 or 13. Contrary to earlier findings, we found no significant impact on incidence of acute GVHD or TRM following Allo-SCT. These differences in results could be due to a lower mutation frequency in the studied population and/or a lower overall incidence of severe GVHD. On the basis of these findings we conclude that a consideration to NOD2/CARD15 mutation status is not pertinent when selecting a donor for Allo-SCT at our centre.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2

Similar content being viewed by others

References

  1. Hugot JP, Chamaillard M, Zouali H, Lesage S, Cézard JP, Belaiche J et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411: 599–603.

    Article  CAS  Google Scholar 

  2. Ogura Y, Bonen DK, Inohara N, Nicolae DL, Chen FF, Ramos R et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411: 603–606.

    Article  CAS  Google Scholar 

  3. Eckmann L, Karin M . NOD2 and Crohn's disease: loss or gain of function? Immunity 2005; 22: 661–667.

    Article  CAS  Google Scholar 

  4. Holler E, Rogler G, Herfarth H, Brenmoehl J, Wild PJ, Hahn JH et al. Both donor and recipient NOD2/CARD15 mutations associate with transplant-related mortality and GvHD following allogeneic stem cell transplantation. Blood 2004; 104: 889–894.

    Article  CAS  Google Scholar 

  5. Holler E, Rogler G, Brenmoehl J, Hahn J, Herfarth H, Greinix H et al. Prognostic significance of NOD2/CARD15 variants in HLA-identical sibling hematopoietic stem cell transplantation: effect on long-term outcome is confirmed in 2 independent cohorts and may be modulated by the type of gastrointestinal decontamination. Blood 2006; 107: 4189–4193.

    Article  CAS  Google Scholar 

  6. Elmaagacli AH, Koldehoff M, Hindahl H, Steckel NK, Trenschel R, Peceny R et al. Mutations in innate immune system NOD2/CARD 15 and TLR-4 (Thr399Ile) genes influence the risk for severe acute graft-versus-host disease in patients who underwent an allogeneic transplantation. Transplantation 2006; 81: 247–254.

    Article  CAS  Google Scholar 

  7. Svahn BM, Remberger M, Myrbäck KE, Holmberg K, Eriksson B, Hentschke P et al. Home care during the pancytopenic phase after allogeneic hematopoietic stem cell transplantation is advantageous compared to hospital care. Blood 2002; 100: 4317–4324.

    Article  CAS  Google Scholar 

  8. Olerup O, Zetterquist H . HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 h: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992; 39: 225–235.

    Article  CAS  Google Scholar 

  9. Glucksberg H, Storb R, Fefer A, Buckner CD, Neiman PE, Clift RA et al. Clinical manifestations of graft-versus-host disease in human recipients of marrow from HLA-matched sibling donors. Transplantation 1974; 18: 295–304.

    Article  CAS  Google Scholar 

  10. Hampe J, Grebe J, Nikolaus S, Solberg C, Croucher PJ, Mascheretti S et al. Association of NOD2 (CARD 15) genotype with clinical course of Crohn's disease: a cohort study. Lancet 2002; 359: 1661–1665.

    Article  CAS  Google Scholar 

  11. Mattsson J, Uzunel M, Remberger M, Ringdén O . T-cell mixed chimerism is significantly correlated to a decreased risk of acute graft-versus-host disease after allogeneic stem cell transplantation. Transplantation 2001; 71: 433–439.

    Article  CAS  Google Scholar 

  12. Ringdén O, Remberger M, Persson U, Ljungman P, Aldener A, Andström E et al. Similar incidence of graft-versus-host disease using HLA-A, -B and -DR identical unrelated bone marrow donors as with HLA-identical siblings. Bone Marrow Transplant 1995; 15: 619–625.

    PubMed  Google Scholar 

  13. Granell M, Urbano-Ispizua A, Aróstegui JI, Fernández-Avilés F, Martínez C, Rovira M et al. Effect of NOD2/CARD15 variants in T-cell depleted allogeneic stem cell transplantation. Haematologica 2006; 91: 1372–1376.

    CAS  PubMed  Google Scholar 

  14. Oh H, Loberiza Jr FR, Zhang MJ, Ringdén O, Akiyama H, Asai T et al. Comparison of graft-vs-host disease and survival after HLA-identical sibling bone marrow transplantation in ethnic populations. Blood 2005; 105: 1408–1416.

    Article  CAS  Google Scholar 

  15. Remberger M, Aschan J, Lönnqvist B, Carlens S, Gustafsson B, Hentschke P et al. An ethnic role for chronic, but not acute graft-versus-host disease after HLA-identical sibling stem cell transplantation. Eur J Haematol 2001; 66: 50–56.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We are greatly indebted to Ms Agneta Stålberg for her invaluable assistance in retrieving patient samples. We also thank the staff at the Centre for Allogeneic Stem Cell Transplantation, and departments of Haematology and Paediatrics for truly competent and compassionate patient care.

Author information

Authors and Affiliations

  1. Centre for Allogeneic Stem Cell Transplantation, Karolinska University Hospital, Stockholm, Sweden

    D Sairafi, O Ringdén & J Mattsson

  2. Department of Clinical Immunology, Institution of Laboratory medicine, Karolinska Institutet, Stockholm, Sweden

    D Sairafi, M Uzunel, M Remberger, O Ringdén & J Mattsson

Authors
  1. D Sairafi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M Uzunel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M Remberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O Ringdén
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J Mattsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D Sairafi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sairafi, D., Uzunel, M., Remberger, M. et al. No impact of NOD2/CARD15 on outcome after SCT. Bone Marrow Transplant 41, 961–964 (2008). https://doi.org/10.1038/bmt.2008.9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/bmt.2008.9

Keywords

This article is cited by

Search

Advanced search

Quick links