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Current status and recent advances of next generation sequencing techniques in immunological repertoire

Genes & Immunity volume 17, pages 153–164 (2016)Cite this article

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Abstract

To ward off a wide variety of pathogens, the human adaptive immune system harbors a vast array of T-cell receptors (TCRs) and B-cell receptors (BCRs), collectively referred to as the immune repertoire. High-throughput sequencing (HTS) of TCR/BCR genes allows in-depth molecular analysis of T/B-cell clones, providing an unprecedented level of detail when examining the T/B-cell repertoire of individuals. It can evaluate TCR/BCR complementarity-determining region 3 (CDR3) diversity and assess the clonal composition, including the size of the repertoire; similarities between repertoires; V(D)J segment use; nucleotide insertions and deletions; CDR3 lengths; and amino acid distributions along the CDR3s at sequence-level resolution. Deep sequencing of B-cell and T-cell repertoires offers the potential for a quantitative understanding of the adaptive immune system in healthy and disease states. Recently, paired sequencing strategies have also been developed, which can provide information about the identity of immune receptor pairs encoded by individual T or B lymphocytes. HTS technology provides a previously unimaginable amount of sequence data, accompanied, however, by numerous challenges associated with error correction and interpretation that remain to be solved. The review details some of the technologies and some of the recent achievements in this field.

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Acknowledgements

This work was supported by funds received from the National Natural Science Foundation of China (No. 81271810, 81571953), 12-5 state S&T Projects for infectious diseases (2012ZX10002-007), Doctoral Fund of Ministry of Education of China (20120101110009), and Zhejiang medical science and technology project (2015118507).

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  1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

    X-L Hou, L Wang, Y-L Ding, Q Xie & H-Y Diao

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Hou, XL., Wang, L., Ding, YL. et al. Current status and recent advances of next generation sequencing techniques in immunological repertoire. Genes Immun 17, 153–164 (2016). https://doi.org/10.1038/gene.2016.9

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