Research ArticleNovel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis
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Supplementary Information accompanies the paper on the Laboratory Investigation website
Akihiko Ito and Takahiro Mimae: These authors contributed equally to this work.
Pseudopodia play a role in cell migration and pseudopodia proteomics may enhance our understanding of malignant phenotypes of tumor cells. A pseudopodia proteomics methodology was developed using an excimer laser and two-dimensional difference gel electrophoresis with ultrahigh sensitive fluorescent dye. Pseudopodia-enriched proteins were identified, and one such protein, RAB1A, is directly involved in pseudopodia formation.
Supplementary information: The online version of this article (doi:10.1038/labinvest.2012.98) contains supplementary material, which is available to authorized users.