Abstract
Dendritic cells can prime naive CD4+ T cells; however, here we demonstrate that dendritic cell–mediated priming was insufficient for the development of T helper type 2 cell–dependent immunity. We identify basophils as a dominant cell population that coexpressed major histocompatibility complex class II and interleukin 4 message after helminth infection. Basophilia was promoted by thymic stromal lymphopoietin, and depletion of basophils impaired immunity to helminth infection. Basophils promoted antigen-specific CD4+ T cell proliferation and interleukin 4 production in vitro, and transfer of basophils augmented the population expansion of helminth-responsive CD4+ T cells in vivo. Collectively, our studies suggest that major histocompatibility complex class II–dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.
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Acknowledgements
We thank M. Mohrs (Trudeau Institute) for 4get mice; A. Troy for critical reading of this manuscript; E. Tait for generating the bone marrow chimeras; and the University of Pennsylvania Flow Cytometry Core and Center for Molecular Studies in Digestive and Liver Diseases Morphology Core for assistance with sorting and immunofluorescence staining. Supported by the National Institutes of Health (Artis lab: AI61570 and AI074878 to D.A., T32 training grant AI007532-08 to J.G.P., F31 training grant GM082187 to S.A.S. and T32 CA09140-30 to B.C.T.; Pearce lab: AI32573 and AI53825; and Laufer lab), the Burroughs Wellcome Fund (Artis lab: D.A.), the National Institute of Diabetes and Digestive Kidney Diseases (Artis lab: DK50306), the Crohn's and Colitis Foundation of America (Artis lab: D.A. and M.G.N.) and the University of Pennsylvania (Artis lab: D.A. and T.M.L.).
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Perrigoue, J., Saenz, S., Siracusa, M. et al. MHC class II–dependent basophil–CD4+ T cell interactions promote TH2 cytokine–dependent immunity. Nat Immunol 10, 697–705 (2009). https://doi.org/10.1038/ni.1740
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DOI: https://doi.org/10.1038/ni.1740
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