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The hESC line Envy expresses high levels of GFP in all differentiated progeny

Abstract

Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.

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Figure 1: Genetic and phenotypic analysis of Envy cells.
Figure 2: Differentiated progeny of Envy cells retain robust GFP expression.

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References

  1. Reubinoff, B.E., Pera, M.F., Fong, C.Y., Trounson, A. & Bongso, A. Nat. Biotechnol. 18, 399–404 (2000).

    Article  CAS  Google Scholar 

  2. Stamp, L., Crosby, H.A., Hawes, S.M., Strain, A.J. & Pera, M.F. Stem Cells 23, 103–112 (2005).

    Article  CAS  Google Scholar 

  3. Soriano, P. Nat. Genet. 21, 70–71 (1999).

    Article  CAS  Google Scholar 

  4. Mao, X., Fujiwara, Y., Chapdelaine, A., Yang, H. & Orkin, S.H. Blood 97, 324–326 (2001).

    Article  CAS  Google Scholar 

  5. Ma, Y., Ramezani, A., Lewis, R., Hawley, R.G. & Thomson, J.A. Stem Cells 21, 111–117 (2003).

    Article  CAS  Google Scholar 

  6. Gropp, M. et al. Mol. Ther. 7, 281–287 (2003).

    Article  CAS  Google Scholar 

  7. Liu, Y.P. et al. Stem Cells Dev. 13, 636–645 (2004).

    Article  CAS  Google Scholar 

  8. Reubinoff, B.E. et al. Nat. Biotechnol. 19, 1134–1140 (2001).

    Article  CAS  Google Scholar 

  9. Goldstein, R.S., Drukker, M., Reubinoff, B.E. & Benvenisty, N. Dev. Dyn. 225, 80–86 (2002).

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Embryonic Stem Cell International (ESI) Pte. Ltd., The Australian Stem Cell Centre, The Juvenile Diabetes Research Foundation and The National Health and Medical Research Council (NHMRC) of Australia. A.G.E. is an NHMRC Senior Research Fellow. M.D. is a Howard Florey Fellow.

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Correspondence to Edouard G Stanley.

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This work was supported by Embryonic Stem Cell International (E.S.I.) Pte. Ltd. who have ownership of the Envy cell line.

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Costa, M., Dottori, M., Ng, E. et al. The hESC line Envy expresses high levels of GFP in all differentiated progeny. Nat Methods 2, 259–260 (2005). https://doi.org/10.1038/nmeth748

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