Abstract
We outline current in vitro and in vivo models for experimental proliferative vitreoretinopathy (PVR) and provide a detailed protocol of our standardized in vivo PVR model. PVR is the leading cause of failed surgical procedures for the correction of rhegmatogenous retinal detachment. The pathogenesis of this multifactorial condition is still not completely understood. Experimental models for PVR help us understand the factors that play a role in the pathogenesis of the disease process in a controlled manner and allow for reproducible preclinical assessment of novel therapeutic interventions. We describe a cell injection model in detail that uses homologous retinal pigment epithelial (RPE) cell cultures to induce PVR over a 2–8 week period.
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Acknowledgements
The authors would like to thank Ernesto Barron for assistance with the figures and Susan Clarke for editorial assistance. This work was supported by National Institutes of Health grant EY02061 and a National Eye Institute core grant to the Doheny Eye Institute (EY03040).
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Agrawal, R., He, S., Spee, C. et al. In vivo models of proliferative vitreoretinopathy. Nat Protoc 2, 67–77 (2007). https://doi.org/10.1038/nprot.2007.4
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DOI: https://doi.org/10.1038/nprot.2007.4
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