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Post-Transplant Events

Early emergence of PNH-like T cells after allogeneic stem cell transplants utilising CAMPATH-1H for T cell depletion

Bone Marrow Transplantation volume 36, pages 237–244 (2005)Cite this article

Summary:

CAMPATH-1H (C-1H) is widely used in vivo and / or in vitro for T cell depletion in hematopoietic SCT. This humanised monoclonal antibody is specific for CD52, a marker coexpressed on the majority of human lymphocytes with CD48 and other glycosylphosphatidyl-inositol (GPI) anchored proteins. We detected CD52 / CD48 dual expression on >99% of CD3+ lymphocytes from normal individuals and all 15 post-SCT patients whose transplants did not utilise C-1H. By contrast, 23 / 26 patients with transplants involving C-1H (in vivo, in vitro or both) exhibited populations lacking CD52 expression that accounted for 49.7% (4.2–86.2%) of the CD3+ lymphocytes (median and range) in samples evaluated at a median of 2 months post-SCT. Most CD52− cells also lacked CD48 expression. These GPI− T cells were of either donor or mixed donor / recipient origin. They were predominant in the early months after SCT at times of profound lymphopenia and inversely correlated with the recovery of the absolute lymphocyte count (r= − 0.663, P<0.0001). The presence of CD52− cells has been correlated previously with clinical outcome after CAMPATH therapy for both malignant and nonmalignant diseases.

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Acknowledgements

This work was funded by a grant from the COGENT trust. We acknowledge the assistance of the nursing staff of the BMT Unit and Oncology Day Beds in obtaining blood samples for use in this study. This work would not have been possible without the agreement of the patients to participate, to whom we are deeply indebted.

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Authors and Affiliations

  1. Department of Pathology and Microbiology, University of Bristol, University Walk, Bristol, UK

    R J Garland, S J Groves, P Diamanti, S E West, K L Winship, N J Goulden & C G Steward

  2. Department of Immunology and Immunogenetics, North Bristol NHS Trust, Bristol, UK

    P F Virgo

  3. Bone Marrow Transplant Unit, Royal Hospital for Children, Bristol, UK

    S P Robinson, A Oakhill, J M Cornish, D I Marks, N J Goulden & C G Steward

  4. Institute for Transfusion Sciences, National Blood Service, Bristol, UK

    P Diamanti & D H Pamphilon

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  1. R J Garland
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Correspondence to R J Garland.

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Garland, R., Groves, S., Diamanti, P. et al. Early emergence of PNH-like T cells after allogeneic stem cell transplants utilising CAMPATH-1H for T cell depletion. Bone Marrow Transplant 36, 237–244 (2005). https://doi.org/10.1038/sj.bmt.1705049

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