Abstract
Apoptosis, a physiological form of cell death, is characterized by the activation of a program that kills cells and recycles their constituents. We have used thymoma cell lines to examine the role of Bcl-2 and caspases in ribosomal destruction during apoptosis. Glucocorticoid- and calcium ionophore (A23187)-induced apoptosis of S49 Neo cells resulted in both 28S rRNA and DNA degradation. Interestingly, anisomycin, a potent protein synthesis inhibitor, also induced 28S rRNA and DNA fragmentation suggesting that the responsible nucleases are present in the viable cells and become activated during apoptosis. The anti-apoptotic protein, Bcl-2, inhibited both glucocorticoid- and anisomycin-induced DNA and 28S rRNA degradation but could not protect against A23187-induced nucleic acid degradation. We next examined the role of caspase activation in the generation of 28S rRNA degradation through the use of ZVAD, a general caspase inhibitor. Under conditions where ZVAD substantially decreased 28S rRNA degradation induced by glucocorticoid or anisomycin, no decrease was observed when A23187 was used to induce apoptosis. Surprisingly, RNA degradation, like DNA degradation, occurs exclusively in shrunken lymphocytes but not those with normal cell volume despite equivalent exposure of the cells to the apoptotic signals. Together, these findings indicate the ribosome is a specific target for death effectors during apoptosis and that a caspase/Bcl-2-independent pathway exists to activate its destruction. Cell Death and Differentiation (2000) 7, 994–1001
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Introduction
Apoptosis participates in many physiological and pathological processes including embryonic development, tissue homeostasis, and shaping of the immune response.1,2 Morphological hallmarks of apoptosis include cell shrinkage, loss of cell-cell contact, and chromatin condensation. During the final stage of apoptosis, the cell breaks into small apoptotic bodies that are phagocytosed by macrophages or neighboring cells, so that, unlike necrosis, no inflammatory response is elicited. Biochemical changes are also distinct and dramatic during apoptosis where the cell systematically destroys vital cellular components through the activation of catabolic enzymes. Several proteases, known as caspases, are being investigated for their role in apoptosis. For example, the interleukin-1-β converting enzyme (ICE)-like family of cysteine proteases plays a pivotal role in cell death.3,4,5,6,7 Interestingly, recent evidence8 indicates that both caspase-dependent and -independent cell death pathways exist in lymphocytes. Additionally, the activation of nucleases leading to the degradation of DNA and RNA has been proposed to be an irreversible commitment step in the induction of apoptosis.9 Indeed, a hallmark feature of apoptosis is internucleosomal DNA degradation that results in the formation of multiples of 180–200 base pair DNA fragments. Thus, the destruction of cellular components by catabolic enzymes plays a major role in apoptosis.
One extensively-studied model system for apoptosis is the glucocorticoid-induced death of immature thymocytes. In this system, treatment of adrenalectomized rats with the synthetic glucocorticoid dexamethasone (dex) causes rapid loss of thymocytes by apoptosis. Within 2 days following steroid treatment, thymic mass is reduced by 50% due solely to the loss of immature thymocytes.10 Primary cultures of thymocytes and most thymoma cell lines also are exquisitely sensitive to glucocorticoids, and the glucocorticoid receptor is required for cytotoxic effects to occur. However, the precise mechanism by which glucocorticoids induce apoptosis is unknown. Thymocytes undergoing glucocorticoid-induced cell death show classic features of apoptosis including general increases in protein, DNA and RNA degradation.11,12,13,14 Steroid-induced RNA degradation occurs rapidly after hormone administration. In dex-treated rat thymocytes, RNA degradation is detectable as early as 2.5 h after steroid treatment and by 4 h there is a 50% increase in RNA degradation.11 Additionally, the ability to synthesize new proteins has been shown to be inhibited during the cell death process.15,16
One of the RNA components of the large ribosomal subunit, 28S rRNA, has been shown to be degraded during apoptosis in a rat myeloid leukemia cell line,17,18 human lymphocytes,19 bovine endothelial cells, as well as primary cultures of prednisone treated rat thymocytes.18 Samali et al,20 have shown that degradation of 28S rRNA occurs in some but not all cell types and can occur independent of DNA fragmentation. Additionally, Houge et al,18 have mapped the cleavage sites on the 28S rRNA molecule in apoptotic IPC-81 cells induced by cAMP to the D2 and D8 regions of the molecule. However, the mechanisms of regulation for RNA degradation are not well understood. We have now used the thymoma cell line, S49, as a model system to further examine the destruction of ribosomal components during apoptosis and the signaling pathways associated with this rRNA degradation. We show that 28S rRNA fragmentation consistently occurs in S49 cells irrespective of the death signal used to initiate apoptosis and in the absence of protein synthesis. Additionally, we show a direct correlation between cell shrinkage and 28S rRNA degradation. Furthermore, Bcl-2 overexpression and inhibition of caspase activity only partially abrogates 28S ribosomal RNA degradation.
Results
DNA and RNA degradation occurs in response to multiple apoptotic agents
The activation of nucleases appears to be a final commitment step in the apoptotic process, and thus, we wanted to examine the effect of nuclease activation on RNA and DNA integrity. Previous studies have shown that the synthetic glucocorticoid, dexamethasone (dex), induces apoptosis in S49 Neo cells.15,21,22 S49 Neo cells were treated with 0.25 μM dex for 24 h and the number of apoptotic cells, determined by microscopic analysis and trypan blue exclusion, increased from 2.7±0.9% to 30.4±4.9%. Nucleases that cleave DNA internucleosomally were also activated demonstrating that the cells were dying by apoptosis (Figure 1A). To determine if apoptotic nucleases degraded RNA as well, total RNA was isolated and separated by non-denaturing agarose gel electrophoresis. The band of intact 28S rRNA was diminished and one prominent, novel band was visible, indicating that 28S rRNA was being cleaved to produce a smaller fragment (Figure 1A). This degradation appeared to occur concomitantly with the apoptotic morphology as judged microscopically and the associated DNA fragmentation. 18S rRNA did not appear to be grossly degraded in either control or glucocorticoid treated cells.
It is conceivable that RNA degradation is part of the steroid signal transduction pathway because these hormones are well known to inhibit protein synthesis in lymphocytes15,16 or, alternatively, RNA degradation may occur independent of the death signal employed. To distinguish between these two possibilities, S49 Neo cells were induced to undergo apoptosis via different stimuli that utilized alternative signal transduction pathways. Treatment of S49 Neo cells with the protein synthesis inhibitor, anisomycin, (1.5 μM, ANIS) or with the calcium ionophore, A23187 (2 μM) resulted in apoptosis as demonstrated by the presence of internucleosomal DNA cleavage (Figure 1A). Because these agents induced death with different kinetics than dex, DNA and total RNA were isolated from treated cells when approximately 30% of the cells were apoptotic as determined by microscopic analysis and trypan blue dye exclusion to ensure that A23187-, anisomycin-, and dex-treated cells were at similar stages of apoptosis. As with glucocorticoid treatment, anisomycin increased the number of apoptotic cells from 2.0±0.6% to 31±1.1%, and A23187-treatment increased the number of apoptotic cells from 2.7±0.3% to 33.1±6.8%. Both agents induced internucleosomal cleavage of DNA and 28S rRNA degradation resulting in the formation of the novel RNA degradation product (Figure 1A). These data suggest that 28S rRNA degradation is not the result of a specific death stimulus but appears to be part of a common apoptotic pathway downstream from signal-specific events. Furthermore, these findings suggest that nucleases responsible for RNA degradation are already present in the cells since these experiments, specifically those using anisomycin, were conducted under conditions where protein synthesis is effectively inhibited.
Degradation of 28S rRNA is partially inhibited by Bcl-2
Bcl-2 expression has been shown to protect S49 cells from dex-induced internucleosomal DNA degradation and apoptosis.15,23 To determine if Bcl-2 had a similar protective effect against RNA degradation, S49 Bcl-2 cells were treated with dex, anisomycin and A23187 and RNA integrity was examined. The level of Bcl-2 in these cells has been shown to be approximately 20-fold greater than background (Huang and Cidlowski, unpublished observation). Consistent with previous studies, the number of apoptotic cells in dex-treated, Bcl-2 expressing cultures did not increase above 5% and no internucleosomal DNA fragmentation was observed, indicating that these cells were resistant to steroid-induced cell death (Figure 1B).15,23 In addition, RNA integrity was examined by agarose gel electrophoresis and the intact 28S rRNA band was not diminished and no novel bands were visible indicating that Bcl-2 expression repressed dex-induced RNA degradation as well (Figure 1B). Thus, dex-induced 28S rRNA degradation appears to be an apoptotic-specific event inhibited by Bcl-2 expression. We also examined the ability of the protein synthesis inhibitor, anisomycin, to induce DNA fragmentation and 28S rRNA degradation in the Bcl-2 cells. As with dex treatment, the number of apoptotic cells in the Bcl-2 expressing cultures did not increase above 5% and neither DNA nor 28S rRNA degradation was visible indicating that Bcl-2 can also protect against anisomycin induced apoptosis (Figure 1B). To determine if S49 Bcl-2 cells are capable of degrading 28S rRNA when undergoing apoptosis, cells were treated with 2 μM A23187. Previous studies have shown that Bcl-2 expression does not completely block A23187-induced apoptosis in S49 cells but confers only partial resistance.15 A23187-treatment increased the number of apoptotic cells from 1.7±0.6% to 30.0±1.3% and induced internucleosomal DNA fragmentation (Figure 1B). Additionally, the 28S rRNA band was degraded in a manner very similar to that observed in apoptotic S49 cells (Figure 1B). Thus, apoptosis and 28S rRNA degradation appear to be tightly coupled. Interestingly, Bcl-2 cells are resistant to dex-induced but not ionophore-induced RNA degradation indicating that the RNA degradation pathway remains intact in the Bcl-2 over-expressing cells. Additionally, this suggests that multiple signaling mechanisms can be employed to activate the catabolic component of the apoptotic machinery.
Selective degradation of 28S rRNA is partially inhibited by Bcl-2
Houge et al,17,18 used Northern blot analysis to map the apoptotic cleavage sites on the 28S rRNA molecule from IPC-81 cells and bovine endothelial cells as well as primary thymocytes. In order to confirm 28S rRNA degradation and compare the patterns of 28S rRNA fragmentation in dex-treated S49 cells to the results obtained by Houge et al, Northern blots of RNA from dex-treated S49-Neo cells were hybridized with probes that recognized the 5′ end of the 28S rRNA. Representative hybridizations are shown in Figure 2. The 5′ probe recognized five prominent fragments approximately 2900, 2500, 550, 200 and 50 bases in size (Figure 2A). These data indicate that ribosomal RNA destruction occurs via a specific, non-random process during the catabolic phase of apoptosis. Anisomycin produced a similar pattern of 28S rRNA degradation in S49 Neo cells. Near total 28S rRNA degradation was observed in A23187-treated S49 Neo cells, however, common degradation products were observed (see arrows). Overall, the pattern of degradation induced via different death stimuli using distinct signal transduction pathways appeared similar suggesting the involvement of a common ribonuclease.
To examine the effects of Bcl-2 expression on 28S rRNA cleavage, S49 Bcl-2 cells were treated with dex, anisomycin, and A23187 and examined for 28S rRNA fragmentation by Northern blot analysis. Analysis of 28S rRNA from dex- and anisomycin-treated S49 Bcl-2 cells resulted in little 28S rRNA fragmentation (Figure 2B). However, treatment of S49 Bcl-2 cells with A23187 resulted in cleavage of the 28S rRNA identical to the pattern observed in the absence of Bcl-2, suggesting the activation of a similar ribonuclease in a Bcl-2-independent apoptotic pathway. Interestingly, the extent of 28S rRNA degradation in S49 Bcl-2 cells was reduced over that observed in the absence of Bcl-2, suggesting that Bcl-2 overexpression can in part repress, but not inhibit, this catabolic pathway.
Caspase dependent/independent 28S rRNA catabolism
Caspases play a pivotal role in executing the apoptotic pathway,3,7 but alternative caspase independent pathways have recently been described.8 To determine if 28S rRNA degradation is dependent on caspase activation, S49 Neo cells were treated with dex or anisomycin in the presence or the absence of the general caspase inhibitor ZVAD for 24 h. Treatment with 75 μM ZVAD decreased the percentage of apoptotic cells from 40.0±8.7% to 23.0±6.9% for dex and decreased the percentage apoptotic cells from 78.6±6.0% to 22.6±6.9% for anisomycin. DNA and RNA were isolated from each treatment and analyzed by gel electrophoresis. Both DNA and 28S rRNA degradation were reduced, but not fully eliminated in the dex plus ZVAD and the anisomycin plus ZVAD treated cells (Figure 3). Consistent with these results, analysis of RNA fragmentation by Northern blot also showed a decrease in the quantity of RNA fragments produced, although the patterns of degradation were remarkably similar. S49 Neo cells treated with specific caspase inhibitors such as DEVD (caspase-3), IETD (caspase-8), and LEHD (caspase-9) in the presence of dex resulted in a small protective effect, however not to the level observed in the presence of the pan-caspase inhibitor ZVAD (data not shown).
To determine if ZVAD had similar affects on A23187 induced apoptosis in S49 Neo and S49 Bcl-2 cells, these cells were treated with 2 μM A23187 alone or in the presence of 75 μM ZVAD. The addition of ZVAD did not substantially block the apoptotic effect of A23187 in either S49 Neo or S49 Bcl-2 cells. Although cell viability was not improved by the addition of the caspase inhibitor, a reduction of both DNA and 28S rRNA degradation was observed in S49 Neo cells upon addition of ZVAD (Figure 4). Interestingly, the combined protective effects of Bcl-2 and ZVAD were not able to rescue cells from the calcium ionophore A23187-induced cell death (Figure 4) suggesting a caspase/Bcl-2-independent pathway for the catabolic effects during apoptosis. Additionally, S49 Neo and S49 Bcl-2 cells treated with specific caspase inhibitors in the presence of A23187 also failed to protect these cells from apoptosis (data not shown).
Most apoptotic agents induce cell death in both a time- and concentration-dependent manner. Therefore, the inability of ZVAD to rescue both S49 Neo and S49 Bcl-2 cells from A23187-induced cell death lead us to examine the protective effect of ZVAD versus alterations in the concentration of the apoptotic agent A23187. S49 Neo and S49 Bcl-2 were treated with 0.1, 0.5, 1, and 2 μM of A23187 for 24 h in the presence and absence of 75 μM ZVAD. Once again, the presence of ZVAD reduced, however did not completely inhibit 28S rRNA fragmentation, even at the lower concentrations of A23187 in S49 Neo cells (Figure 5). These data are similar to the results observed for 2 μM A23187 as shown in Figure 4. Both 1 and 2 μM A23187-induced 28S rRNA degradation in S49 Bcl-2 expressing cells, which again was not inhibited by ZVAD. Interestingly, lower concentrations of A23187 failed to induce 28S rRNA degradation in the Bcl-2 expressing cells, implying that this anti-apoptotic protein is functioning at some level to inhibit ionophore induced apoptosis.
RNA catabolism occurs only in shrunken cells
To date, all cells examined, including those used in these studies, shrink when undergoing apoptosis (for example, see24), and only shrunken cells exhibit DNA degradation and effector caspase activation.25 To assess if cell shrinkage is coupled to 28S rRNA degradation, S49 Neo cells were induced to undergo dex-induced apoptosis, then shrunken and non-shrunken cells were sorted by flow cytometry (Figure 6A). RNA was then isolated from each population and analyzed by agarose gel electrophoresis. Degraded 28S rRNA was visible only in the pre-sorted and shrunken populations, but not the normal, non-shrunken cell population (Figure 6B). This suggests that only the shrunken population of cells degrade their RNA even though all of the cells were exposed to this apoptotic stimuli for an equivalent duration.
Discussion
The present study has demonstrated that ribonucleic acids are degraded under a variety of apoptotic conditions during thymocyte cell death. Additionally, specific fragmentation of 28S rRNA occurs only in the shrunken apoptotic population of cells. Different apoptotic death signals in lymphocytes induced similar patterns of 28S rRNA degradation; however, A23187-treatment led to greater degradation of intact 28S rRNA and the accumulation of smaller 28S rRNA fragments suggesting that degradation products are subsequently broken down into smaller pieces. The fact that 28S rRNA degradation could also be induced by a protein synthesis inhibitor suggests that, like other apoptotic effector molecules, enzymes responsible for 28S rRNA degradation are already present in cells and become active when apoptosis is induced. Bcl-2 expression provided resistance to 28S rRNA degradation during glucocorticoid- and anisomycin-induced cell death but only partially affected death induced by the calcium ionophore A23187. 28S rRNA degradation induced by dex or anisomycin was reduced by caspase inhibition suggesting that caspases play an important role in RNA cleavage under these apoptotic conditions. However, caspase inhibition had little effect on either DNA or 28S rRNA degradation induced by A23187, even at various concentrations of this stimulus. These observations strongly suggest that a caspase/Bcl-2-independent pathway exists for the activation of cell death catabolism.
Regardless of the death stimulus employed, the pattern of 28S rRNA fragmentation was remarkably similar. In most cases, the 5′ probe recognized five degradation products, indicating that specific sites on the RNA molecule were being cleaved. Houge et al,18 have used Northern blot analysis, primer extension, and S1 nuclease protection to map cleavage sites in the 28S rRNA molecule in apoptotic IPC-81 cells with similarities apparent between our results and those reported previously. We both detected a 550 base and a 2900 base fragment recognized by a probe complimentary to the 5′ end of the 28S rRNA. However, unlike our results, Houge et al,18 did not detect 2500, 200 and 50 base bands with their 5′ probe. These differences probably reflect the different probes used, differences in the percentage of apoptotic cells at the time of RNA isolation, and different death stimuli employed, all of which we have shown affect the extent of fragmentation and size of fragments produced.
Because 28S rRNA is a component of the ribosome, it is possible that degradation of this molecule inhibits protein synthesis. Indeed, it is widely recognized that glucocorticoid treatment of thymocytes results in a decrease in protein synthesis in addition to triggering death.15,16 This biochemical parameter can be separated from the apoptotic process by expression of Bcl-2 in that dex-treated S49 Bcl-2 cells, which show a decrease in protein synthesis, show no cell death.15 We have demonstrated that dex-treatment of S49 Bcl-2 cells does not result in significant degradation of 28S rRNA after 24 h. If 28S rRNA degradation is solely responsible for the glucocorticoid-induced inhibition of protein synthesis then 28S rRNA degradation should also occur in dex-treated S49 Bcl-2 cells. These results strongly suggest that 28S rRNA degradation is not responsible for steroid-induced protein synthesis inhibition, and RNase activity is tightly coupled to glucocorticoid-induced apoptosis but not to other glucocorticoid-mediated effects. In contrast to the S49 Bcl-2 cell response to dex-treatment, A23187 does induce apoptosis and 28S rRNA degradation suggesting that more than one pathway can activate 28S rRNA degradation.
Cell shrinkage and activation of caspases are two distinct features of apoptotic cells suggesting a fundamental role for these two processes during apoptosis. In this study, we show that only shrunken cells exhibit 28S rRNA degradation. Additionally, ionic fluxes that induce cell shrinkage during apoptosis have been shown to be required to activate the nuclease responsible for this degradation.25,26 Inhibition of caspase activity reduced DNA and 28S rRNA degradation during glucocorticoid- and anisomycin-induced apoptosis in S49 Neo cells suggesting that RNA degradation is a caspase-dependent process. However, the addition of ZVAD to either S49 Neo or S49 Bcl-2 cells treated with A23187 only minimally alters the extent of DNA or 28S rRNA degradation. These data indicate that a caspase/Bcl-2-independent pathway exists for the activation of apoptosis.
The nucleases responsible for cleavage of both DNA and RNA have yet to be completely identified. However several potential candidates have been proposed as the apoptotic nuclease responsible for internucleosomal DNA cleavage including DNase I, Dnase II and NUC18. Recently, a caspase-dependent nuclease was isolated from mouse T-cell lymphoma cells designated CAD.27,28 Cytosolic extracts from normal, healthy cells were shown to contain nuclease activity upon treatment with caspase 3, suggesting that activation of the apoptotic nuclease is a caspase-dependent process. This occurs by the ability of caspase 3 to inactivate an inhibitor protein, ICAD permitting the translocation of CAD to the nucleus. In our study, the pan caspase inhibitor ZVAD was shown to substantially decrease both DNA and rRNA degradation in dex and anisomycin treated S49 Neo cells. However, DNA and rRNA degradation in cells treated with the calcium ionophore A23187 were only partially inhibited in the presence of ZVAD. These observations suggest that other nucleases may be involved in the apoptotic process perhaps dependent on the specific stimulus applied to induce death.
Activation of catabolic enzymes appears to be inherently important in the apoptotic process. 28S rRNA degradation has been found in cAMP-induced apoptosis of a rat myeloid leukemia cell line,17,18 γ-ray-induced apoptosis of human lymphocytes,19 in apoptotic bovine endothelial cells, and in primary cultures of prednisone-treated rat thymocytes,18 as well as systems described in this current study. In other systems, rRNA degradation is not associated with apoptosis suggesting that 28S rRNA cleavage is a cell type-dependent process. Several theories have been proposed for the role of RNA degradation in cell death. First, RNA turnover may be used to destroy RNA encoding proteins that antagonize the apoptotic process.29,30 Second, RNA degradation may be used to ensure that all new transcripts of the dying cell are eliminated making the apoptotic process irreversible.11 Third, RNA turnover may have the general role of breaking down cellular components to facilitate recycling after apoptotic cells are phagocytosed.31 Additionally the studies by Mondino and Jenkins31 have shown that there is an accumulation of cytosolic RNA-binding proteins in apoptotic cells. Several of these binding proteins recognize AU-rich sequences known to influence RNA stability. Furthermore, accumulation of these proteins occurs concomitantly with an increase in RNA turnover and apoptosis. These results suggest that degradation of RNA requires activation of a nuclease and also a possible role for accessory RNA-binding proteins. This multiple step process may provide a mechanism to target specific RNA molecules for degradation. Because 28S rRNA degradation does not appear to affect protein synthesis or any cellular process, it is possible that this molecule is not the primary target of the apoptotic RNase. Therefore, it will be important to establish whether other substrates exist and how they participate in the apoptotic process.
Materials and Methods
Materials
RPMI medium and TriZol reagent were purchased from Gibco-BRL (Gaithersburg, MD, USA) and fetal bovine serum from Irvine Scientific (Santa Ana, CA, USA). Dexamethasone was purchased from Steraloids (Wilton, NH, USA), anisomycin from Sigma Chemical Co. (St. Louis, MO, USA) and A23187 was purchased from Calbiochem (La Jolla, CA, USA). The general caspase inhibitor ZVAD was obtained from Kamiya (Seattle, WA, USA). All other reagents used were of the highest grade available from general suppliers.
Cell culture
The mouse thymoma cell lines, S49 Neo and S49 Bcl-2, are stable, clonal transformants of the parental S49.1 cell line. These cell lines were created by stably infecting S49.1 cells with recombinant amphotrophic retrovirus carrying the neomycin resistance gene alone or in combination with a bcl-2 complementary DNA as described previously.23 Both cell lines were maintained at 37°C, 7% CO2 atmosphere in logarithmic growth (105–106 cells/ml) in RPMI supplemented with 10% heat-inactivated fetal bovine serum, 4 mM glutamine, 100 U/ml penicillin, and 75 U/ml streptomycin. Agents used to induce death include dexamethasone (dex), anisomycin and A23187. ZVAD was used to inhibit caspase activity. Because different death signals have different kinetics for inducing apoptosis, treatments were selected so that, for most experiments, 30–40% of the cells were morphologically apoptotic as judged by light microscopy and trypan blue dye exclusion.
Cell sorting
S49 Neo cells were treated with 0.25 μM dexamethasone for 42 h then stained with propidium iodide (PI) to eliminate cells that have lost their membrane integrity from further analysis. Simultaneous sorting of both the normal and shrunken viable populations of cells was accomplished using a Becton Dickinson FACSVantage equipped with CELLQuest software. Gates were set on a forward-scatter versus PI fluorescence dot plot. Cells were sorted into 5-ml tubes containing approximately 300 μl of RPMI-1640 media and immediately processed for RNA isolation.
Preparation and analysis of DNA and RNA
For DNA isolation, 1×107 cells were pelleted, lysed in 5 mM Tris-HCl, pH 8.0, 20 mM EDTA, 0.5% Triton X-100 and 0.1% SDS, and treated with 0.4 mg/ml proteinase K for 1 h at 55°C. Lysates were extracted once with phenol-chloroform-isoamyl alcohol (25 : 24 : 1, by volume) and once with chloroform-isoamyl alcohol alone. One hundred mM NaCl was added and DNA precipitated by addition of two volumes of 100% ice cold ethanol at −80°C for 2 h. The suspension was centrifuged at 18 000×g for 30 min at 4°C. DNA pellets were air dried and resuspended in 10 mM Tris-HCl, pH 7.4, 1 mM EDTA, containing 0.3 mg/ml DNase-free RNase A and incubated overnight at 37°C. Fifteen μg DNA was loaded on 1.8% agarose gels that were subsequently stained with ethidium bromide. Total RNA was prepared from 2×107 cells using guanidine isothiocyanate and phenol.32 Two μg of total RNA was heated to 65°C and immediately loaded onto 1% non-denaturing gel that was subsequently stained with ethidium bromide.
Northern blot analysis
Total RNA was denatured using glyoxal and dimethyl sulfoxide and 2–3 μg RNA was separated by electrophoresis through 1.8% agarose gels. RNA was transferred to nylon membranes as previously described.33 Filters were hybridized in a buffer containing 5×SSPE, 2×Denhardt's, 0.5% SDS, 100 μg/ml denatured and sheared salmon sperm DNA and 5′ end 32P-labeled oligonucleotide (1×106 c.p.m./ml hybridization fluid) complementary to positions 16–51 (5′ probe) of the rat 28S rRNA.
Abbreviations
- ANIS:
-
anisomycin
- dex:
-
dexamethasone
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This research was supported by the NIEHS Intramural Program.
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King, K., Jewell, C., Bortner, C. et al. 28S ribosome degradation in lymphoid cell apoptosis: evidence for caspase and Bcl-2-dependent and -independent pathways. Cell Death Differ 7, 994–1001 (2000). https://doi.org/10.1038/sj.cdd.4400731
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DOI: https://doi.org/10.1038/sj.cdd.4400731
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