Abstract
Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general ‘eat me’ signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-β. Furthermore, the results of time-lapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.
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Main
Apoptosis is an essential physiological process for embryonic morphogenesis and for the maintenance of homeostasis in adult multicellular organisms.1 Rapid and efficient cell corpse clearance is crucial to the protection of normal healthy cells from harmful contents and the debris of dying cells, and such clearance also reduces the tissue damage resulting from inappropriate inflammation or autoimmune responses.2 Clearance is generally achieved via the activity of professional phagocytes (macrophages and immature dendritic cells) and neighboring cells (fibroblasts, endothelial, and epithelial cells). The central element in the recognition process is the cell surface presentation of adhesive engulfment ligands (also referred to as ‘eat me’ signals) displayed by apoptotic cells.3, 4 Although little remains known regarding the molecular identities of engulfment ligands, the best-characterized marker of apoptotic cells is phosphatidylserine (PS) exposure, which has been associated with losses in phospholipid asymmetry.
Several classes of receptors have been implicated in the recognition of apoptotic cells, possibly via direct binding to the exposed PS or indirect binding through bridging molecules.3 These receptors include the phosphatidylserine receptor (PSR),5 C. elegans CED-1,6 CD14,7 the αvβ3 and αvβ5 integrins,8 and the receptor tyrosine kinase Mer.9 In addition, several scavenger receptors have been theorized to mediate the binding and uptake of aged/apoptotic cells, including LOX-1,10 CD36,11 Drosophila Croquemort,12 and low-density lipoprotein (LDL)-receptor-related protein (LRP1, also referred to as CD91).13, 14 These receptors may function in parallel or redundant phagocytic systems, as the blocking of any receptor impairs apoptotic cell engulfment only partially.15 Although these receptors have been implicated in the engulfment of aged/apoptotic cells, no evidence has yet been presented to show that any of these receptors can directly recognize PS. Although the above-mentioned bridging molecules have been demonstrated to recognize PS, their activity is insufficient to explain all of the observed responses to PS, as well as the attendant anti-inflammatory and anti-immunogenic effects. PSR was originally shown to be a specific PS receptor, but now it appears not to perform this function. Therefore, a membrane receptor for PS remains to be identified.16
Stabilin-2 is a large multifunctional receptor, which harbors seven FAS1 domains, four EGF-like repeats, and a link domain. Stabilin-2 was initially identified as the endocytic hyaluronan receptor on rat liver sinusoidal endothelial cells17, 18 and has also been reported to function as a scavenger receptor, which effects the endocytosis of modified LDL and glycation end products.19, 20 Stabilin-2 also has been shown to bind to Gram-negative and Gram-positive bacteria, thereby suggesting the potential involvement of stabilin-2 in the immune response. It is expressed at high levels in the sinusoidal endothelial cells of the spleen, lymph nodes, and liver.18, 21 In this study, we present evidence that stabilin-2 is also expressed in human monocyte-derived macrophages (HMDMs). These findings compelled us to speculate that stabilin-2 may participate in the recognition and engulfment of apoptotic and aged cells in macrophages. Accordingly, the potential for stabilin-2 to function as a PS receptor in the clearance of aged and apoptotic cells is addressed and supported by the evidence reported herein.
Results
Stabilin-2 performs a crucial function in the rapid engulfment of aged RBCs
Stabilin-2 has been identified as a multifunctional scavenger receptor that endocytoses modified LDL and glycation end products and also evidences Gram-negative and Gram-positive bacterial-binding activities.19, 20 However, the possibility that stabilin-2 might participate in the phagocytosis of PS-exposed cell corpses remained an open question until now. In an effort to assess the potential involvement of stabilin-2 in the recognition and engulfing of apoptotic and aged cells, stabilin-2 was stably transfected into mouse fibroblast L cells, which evidence no phagocytic activity under normal conditions. L cells have been shown not to express any cadherins22 and they have the advantage of being able to distinguish stabilin-2-specific binding from other interactions, because they do not interact significantly with any of the normal or apoptotic cells employed in this study. Through selection in G418, four lines were acquired that consistently expressed stabilin-2 on the cell surface (Figure 1a). We selected aged red blood cells (RBCs) as target cells, because they do not bind to phagocytes without apoptotic signals, and allow for distinction between binding and engulfment via hypotonic lysis.23 Stabilin-2 stably expressing L cells (L/Stab-2) were shown to bind to and engulf aged RBCs (79.4±5.9 and 41.4±4.8, respectively) to a significant degree, whereas L cells transfected with the empty vector (L/Mock) evidenced little binding to and absolutely no phagocytosis of aged RBCs (13.4±4.5 and 2.0±0.7, respectively) (Figures 1b and c). The interaction between RBCs and L/Stab-2 cells was specific to aged cells, as the normal RBCs did not interact with the L/Stab-2 cells in this assay (Figure 1c). To verify the engulfment of aged RBCs, the phagocytosis assay was modified via the pre-labeling of the target cells with phosphatidylcholine (PC)-7-nitrobenz-2-oxa-1,3-diazole (NBD). Distinctly labeled target cells (green) were detected within the L/Stab-2 cells (red) (Figure 1d). Pretreatment with a monoclonal anti-stabilin-2 antibody (mAb 5G3) before exposure to aged RBCs resulted in a substantial reduction in the binding and uptake of aged RBCs (from 78.8±11.1 and 42.1±3.9 to 15.8±5.3 and 17.7±6.5, respectively) (Figure 1e). This antibody is specific to stabilin-2 and does not crossreact with orthologous and paralogous proteins (Supplementary Figure 1 is available online at http://www.nature.com/cdd/index.html). We also evaluated the interaction occurring between L/Stab-2 cells and aged RBCs via time-lapse video microscopy. Aged RBCs were quickly engulfed by L/Stab-2 cells (Figure 1f and Supplementary Movie 1). To further investigate the velocity of uptake, the images were analyzed at shorter intervals. We observed that the uptake of tethered RBCs occurred within 2–4 s (Figure 1g and Supplementary Movie 2).
In an effort to further determine the potential involvement of stabilin-2 in cell corpse removal, we designed three short hairpin RNAs (shRNA)-targeting stabilin-2 (Figure 2a) and cloned them into the pSuper vector. The suppression of stabilin-2 expression was evaluated in CHO-K1 cells via the co-transfection of the shRNAs along with a stabilin-2-expressing vector. The expression of stabilin-2 was suppressed profoundly by shRNA2, but only marginally by shRNA1 and shRNA3 (Figure 2b). To suppress effectively the expression of stabilin-2, lentiviruses encoding for stabilin-2 shRNA2 (shStab-2) or its scrambled shRNA (shCont) along with green fluorescent protein (GFP) were prepared (Figure 2c). Under similar transfection efficiency conditions, shRNA against stabilin-2, but not control shRNA, downregulated the expression of the stabilin-2 protein (Figure 2d) and significantly reduced the binding and uptake of aged RBCs (from 75.6±7.3 and 44.2±7.4 to 31.4±10.1 and 21.6±4.3, respectively) (Figure 2e). Collectively, our results indicate that stabilin-2 specifically mediates the binding and engulfment of aged RBCs.
Stabilin-2 recognizes PS and engulfs aged RBCs in a PS-specific manner
Although the molecular identities of engulfment ligands remain largely unknown, PS externalization has been identified as a hallmark of the earliest stages of the apoptotic process.24, 25 The exposure of PS to the surface of aged RBCs increased in a time-dependent manner (Figure 3a, left panel). These changes were accompanied by an increase in binding to L/Stab-2 cells (Figure 3a, right panel), thereby indicating that stabilin-2 may recognize PS on the surfaces of aged RBCs. We then evaluated the affinity of fluorescence-labeled liposomes harboring a variety of phospholipids to monolayers of L/Stab-2 cells. As is shown in Figure 3b, PS liposomes bound to L/Stab-2 cells to a significant degree, but other phospholipid liposomes did not. None of the phospholipid liposomes bound to the L/Mock cells to any significant extent. We evaluated the engulfment of aged RBCs by L/Stab-2 cells in the presence of PS liposomes, using PC liposomes as a negative control. The PS liposomes effected a dose-dependent inhibition of engulfment of aged RBCs by L/Stab-2 cells, whereas the PC liposomes exerted no such effects (Figure 3c). The inhibition of aged RBC binding and uptake was restricted to the PS liposomes, and other anionic and neutral phospholipids exerted no detectable effects (Figure 3d). To confirm the specific recognition of PS, we assessed the inhibitory action of a structural analogue of PS using phospho-L-serine (PLS), with phospho-D-serine (PDS) employed as a negative control. Similarly, binding and uptake were inhibited by PLS, but not by PDS (Figure 3e). These findings show that L/Stab-2 cells do not simply interact with aged RBCs through their negative charge, but that they recognize PS stereospecifically on the surfaces of the aged cells.
Stabilin-2 engulfs apoptotic cells via the recognition of PS on the surfaces of apoptotic cells
We conducted phagocytosis assays using apoptotic cells as target cells. Jurkat T and U937 cells were induced to undergo apoptosis via treatment with anti-Fas antibody or etoposide, respectively. Apoptosis was quantified via the binding of fluorecein-5-isothiocyanate (FITC)-labeled annexin V to externalized PS, and was confirmed by staining with propidium iodide (PI), which cannot permeate the intact plasma membranes of apoptotic cells (Figure 4a). As had been expected, the L/Stab-2 cells engulfed the apoptotic cells (Figures 4b and c), and this uptake was inhibited significantly by mAb 5G3 and the PS liposome, thereby suggesting that the PS-specific engulfment by stabilin-2 did not depend on the type of cells or the initiating signals (Figure 4c). We also evaluated the engulfment of apoptotic cells by L/Stab-2 cells using time-lapse video microscopy. Phagocytosis was delayed until ∼5 min (Figure 4d and Supplementary Movie 3), and the elongation of ingestion time may be resultant from the different size of the target cells. We then utilized Jurkat T cells coated with PS in order to directly assess the role of stabilin-2 as a PS receptor involved in the binding and engulfment of apoptotic cells. As is shown in Figure 5a, these cells acquired the ability to bind to annexin V. Viable Jurkat T cells coated with PS in the outer leaflet were engulfed by L/Stab-2 cells, in a manner comparable to that of apoptotic cells (31.2±5.2 and 35.7±10.2, respectively) (Figure 5b). Viable cells coated with PC were not engulfed (Figure 5b). When the PS-treated viable cells were cultured for 6 h after the PS liposomes were washed away, they no longer bound to annexin V, and were therefore not engulfed (Figures 5c and d), thereby indicating that PS liposome treatment did not induce the apoptosis of viable cells. These results indicate that PS alone is sufficient to mediate the tethering or engulfment of apoptotic cells by stabilin-2.
Stabilin-2 is involved in the clearance of aged/apoptotic cells and the production of anti-inflammatory cytokine by HMDMs
To determine whether stabilin-2 is expressed in macrophages, which perform a crucial function in the uptake of apoptotic cells as professional phagocytes, we conducted RT-polymerase chain reaction (PCR) using two different probes and determined that stabilin-2 is expressed in HMDMs, human alveolar macrophages (HAMs), and several macrophage cell lines, including phorbol myristate acetate (PMA)-treated THP-1 and P388D1 cells (Figure 6a). In an effort to assess the functional role of stabilin-2 in a more physiological context, we conducted experiments using HMDMs. In our flow cytometry analyses, we verified that stabilin-2 shRNA, but not control scrambled shRNA, caused a significant reduction in the levels of stabilin-2 expression on the surfaces of HMDMs (Figure 6b). The engulfment of aged RBCs was more pronounced in cases in which HMDMs were utilized as phagocytes. In this case, the percentage of the cells that engulfed aged RBC was approximately 60%, and half of them harbored more than five RBCs. The downregulation of stabilin-2 significantly attenuated the uptake of aged RBCs in CD68+ HMDMs (Figure 6c). The percentage of CD68+ HMDMs that did not engulf RBC after co-culturing with aged RBCs increased from 38.6±3.9 to 55.4±5.5, and the percentage of the HMDMs engulfing more than five RBCs decreased significantly from 29.9±3.2 to 10.4±2.1 (Figure 6d). We attempted to determine whether monoclonal antibody against human stabilin-2 (mAb 5G3) inhibits the engulfment of aged RBCs and apoptotic Jurkat T cells. mAb 5G3 also inhibited the percentage of HMDM engulfing aged RBC (from 59.3±3.8 to 44.5±5.3) (Figure 6e), and the percentage of the HMDMs engulfing more than five RBCs was reduced more efficiently, to the same degree as was observed with stabilin-2 shRNA (data not shown). When apoptotic Jurkat T cells were used as target cells, the HMDMs engulfed 1–3 apoptotic cells, and the reduction of ingested cells may be the result of the different size of the target cells. The percentage of macrophages that engulfed apoptotic cells was inhibited from 29.4±4.1 to 11.8±1.5 in the presence of mAb 5G3, similar to that induced by the PS liposome (Figure 6e). However, the blockade of the stabilin-2 receptor with shRNA or mAb 5G3 induced a partial inhibition of aged and apoptotic cell engulfment. This may be the result of significant redundancy in apoptotic cell recognition, the result of using many macrophage receptors simultaneously.3
An important issue with regard to the uptake of apoptotic cells is the consequential resolution of inflammatory responses.2 The potential anti-inflammatory effects of the recognition and uptake of apoptotic cells may explain the quiet, non-inflammatory nature of apoptotic cell removal during development and tissue remodelling. As the PS-dependent ingestion of apoptotic cells is associated with the production of anti-inflammatory cytokines,26 we attempted to evaluate the potential for stabilin-2 to promote the production of the anti-inflammatory cytokine, transforming growth factor-β (TGF-β), using HMDMs isolated from normal human donors. As was observed with aged cells and PS-containing liposomes, the pretreatment of the cells with anti-stabilin-2 antibody (5G3) resulted in increased TGF-β production (Figure 7a). Similar to what was observed with HMDM, mAb 5G3 also resulted in increased TGF-β production in the L/Stab-2 cells (Figure 7b). Thus, these results provide evidence that stabilin-2 performs an important function in the uptake of aged cells and delivers a ‘tickling signal’, which modulates the immune response via the secretion of anti-inflammatory cytokines.
Discussion
The externalization of PS that is normally observed within the inner leaflet of the cell membrane occurs during apoptosis, and is the best characterized event that can be used to distinguish live cells from dead ones.27 For the rapid and efficient clearance of apoptotic cells, phagocytes must recognize PS on the apoptotic cell surface via either the PS receptor or secreted bridging molecules. Although soluble bridging molecules such as MFG-E88 have been shown to mediate the PS-dependent engulfment of apoptotic cells, membrane receptors that directly recognize PS remain to be identified. Several membrane receptors have been demonstrated to be involved in the clearance of apoptotic cells, including the PSR,5, 28 CD14,7 and scavenger receptors including CD36.11 Among these, the PSR is the most salient representative, as it can recognize PS in a stereospecific manner.5 However, recent studies suggest considerable ambiguity regarding the localization of PSR on the cell surface29 and whether it is crucial for the clearance of apoptotic cells.30, 31 The results of previous studies have demonstrated that CD14 and CD36 are involved in the phagocytosis of apoptotic cells as membrane receptors in HMDMs. However, CD14 appears unlikely to bind preferentially to apoptotic-cell-associated PS, and does not function as a PS-receptor on HMDMs.32 It remains unclear as to whether CD36 mediates the engulfment of apoptotic cells via direct binding to PS.11 The results of the present study suggest that stabilin-2 is a strong candidate for a PS-specific membrane receptor. Four lines of evidence bolster the proposition that stabilin-2 is involved in the clearance of apoptotic cells in human macrophages. First, Stabilin-2-expressing cells were shown to mediate the binding and engulfment of aged and apoptotic cells, and this binding and engulfment activity was inhibited significantly by anti-stabilin-2 antibody or stabilin-2-specific shRNA. Second, stabilin-2 recognizes PS in a stereospecific manner. Third, the blockade of stabilin-2 with shRNA or mAb 5G3 resulted in a reduction in phagocytosis in primary HMDMs. Although the blockade of stabilin-2 receptor partially inhibited the percentage of macrophages engulfing aged RBC, by ∼25%, the percentage of the macrophages engulfing more than five aged RBCs was reduced more efficiently, by approximately 65%. Fourth, the introduction of PS into the outer leaflets of viable cells substantially induced their uptake by stabilin-2, thereby suggesting that stabilin-2 may function as a tethering receptor, which potentially binds directly to PS. Thus, our results indicate that stabilin-2 performs an important function as a PS receptor for cell corpse clearance in macrophages.
In addition to PS-recognizing molecules, the engulfment of apoptotic cells requires the coordinated activity of several cell-surface receptors, which ultimately mediate the internalization of bound apoptotic cells. Therefore, two steps, referred to as ‘tethering’ (recognition of PS) and ‘tickling’ (internalization of apoptotic cells and activation of downstream signalling pathways) are required for the clearance of apoptotic cells. The evidence collected thus far suggests that a host of membrane receptors, including the vitronectin receptor, CD36, and CD14, function in the tethering of apoptotic cells to phagocytes, and that a tickling signal is generated as the result of interactions between PS and the PS-receptor.33 The findings of recent studies have shown that the Mer receptor functions as a tickling receptor.34 Does stabilin-2 simply provide tethering signals or does it deliver both signals? The tickling step involves the internalization of tethered cellular corpses and the induction of an active anti-inflammatory response in phagocytes, following binding to the cellular corpse. In this study, we have determined that the blockade of stabilin-2 blocks the interaction between apoptotic cells and phagocytes. Although stabilin-2 expression may have generated the expression of additional ‘tickling receptors’, the activation of stabilin-2 with the mAb stimulates the production of TGF-β in HMDMs and stabilin-2-expressing L cells. Thus, our data indicate that stabilin-2 may aid in the resolution of inflammation by contributing to both the clearance of apoptotic cells and the secretion of anti-inflammatory cytokines, and thus may prove to be responsible for both the tethering and the tickling of phagocytes.
Only few reports have thus far addressed the velocities of apoptotic cell engulfment in phagocytes. Microglial cells phagocytosed an apoptotic cerebellar granule neuron within 1–2 min.35 IgG-opsonized erythrocytes have been shown to be ingested more slowly (5–7 min) by Raw264.7 macrophages.36 In this study, we determined that aged RBC was ingested quickly, within 2–4 s. For the rapid and efficient clearance of apoptotic cells, a single PS receptor with both tethering and tickling functions on the surface of phagocytes is likely required. In this regard, stabilin-2, which is capable of quickly ingesting apoptotic cells, is strongly suggested to be a key molecule for the clearance of apoptotic cells without causing inflammation. However, in our system, apoptotic cell engulfment was delayed for ∼5 min. The difference in ingestion times between the aged RBCs and the apoptotic cells may be explained by the sizes of the target cells or by differences in engulfment mechanisms. According to our results, macrophages engulfed many numbers of aged RBCs (3–15 cells per cell) as compared with what was observed with the apoptotic cells (1–3 cells per cell). It remains possible that the engulfment of apoptotic cells may require another complication to the current simple model, in which PS exposed on the cell surface functions as a direct target for recognition by stabilin-2. Recently, it has been suggested that calreticulin may function as a second general recognition ligand, via binding and activating LRP or CD91 on engulfing cells.14 Arur et al.37 have suggested that annexin I may function in the facilitation of or enhancement of the recognition of PS by the receptor. Whether calreticulin or annexin I is related to stabilin-2-mediated phagocytosis is currently unclear, and will require further study.
Unlike other molecules, which are expressed ubiquitously, stabilin-2 is expressed in specific cell types, including sinusoidal endothelial cells of the spleen, lymph node, and liver,21 as well as macrophages. It has been reported in previous studies that stabilin-2 is only barely detectable in monocytes/macrophages.20 However, we have determined, via RT-PCR, that stabilin-2 is expressed in HMDMs, HAMs, and several macrophage cell lines, including PMA-treated THP-1 and P388D1. Stabilin-2 was also detected in HMDMs via flow cytometry using mAb 5G3, and its expression was specifically downregulated by shRNA against stabilin-2. This discrepancy may be explained by possible differences in antibody epitopes and RT-PCR conditions. In addition, a few studies have demonstrated that stabilin-1, the closest homologue of stabilin-2, is expressed in macrophages.19, 21 Stabilin-1 and stabilin-2 have similar domain structures and expression patterns, and also operate as scavenger receptors.17, 19, 20 In this point, stabilin-1 may be also involved in the clearance of apoptotic cells in macrophages. It remains to be determined whether two proteins are functionally linked.
In summary, our results demonstrate for the first time that stabilin-2 is a novel PS receptor that provides tethering and tickling signals, and therefore performs a crucial role in the rapid clearance of aged and apoptotic cells and the resolution of inflammation. The identification of stabilin-2 as a receptor that directly recognizes PS on the surfaces of apoptotic cells would facilitate the elucidation of the molecular mechanisms by which apoptotic cells are recognized and phagocytosed in the human body.
Materials and Methods
Reagents
mAb directed against c-Myc (clone 9E10) was obtained from Santa Cruz. mAb directed against CD68 (clone KP1) was purchased from Abcam. mAb directed against Fas was purchased from Upstate. Alexa Fluor 568 or 647-conjugated goat anti-mouse IgG antibodies were obtained from Molecular Probes. Etoposide was acquired from Sigma. Human and mouse spleen cDNAs were obtained from Invitrogen. All phospholipids were purchased from Avanti Polar Lipids.
Cell culture
Human monocytes were obtained from normal donors, isolated using monocyte isolation kit II systems (Milteny Biotec.) and cultured in X-vivo 10 (BioWhitaker) containing 10% human serum. The differentiated macrophages (HMDMs) were utilized at 7–10 days of culture. Mouse fibroblast L cells (ATCC CCL-1) were generously donated by Dr M Takeichi (Kyoto University, Japan), and were grown in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% heat-inactivated FBS and the appropriate antibiotics. U937, a human monocytic cell line, and Jurkat T, a human leukemic cell line, were maintained in RPMI-1640 medium containing 10% FBS.
Production of mAb
To generate monoclonal anti-human stabilin-2 antibodies, a His-tagged recombinant protein corresponding to amino acids 1173-1727 was expressed in E. coli and purified with Ni-NTA resin, in accordance with the manufacturer's instructions. Six mice were immunized with 20 μg of purified recombinant protein each, at 2-week intervals. All mAb production was performed by Dinona Inc. (Seoul, Korea). Supernatants resulting from the fusion were tested by flow cytometry for L cells transfected with stabilin-2 cDNA or empty vector. One hybridoma supernatant (clone 5G3, IgG1, lambda chain) was selected. This mAb (5G3) was shown to be reactive via flow cytometry and immunohistochemical staining, and did not crossreact with orthologous proteins including mouse and rat stabilin-2, nor with paralogous proteins such as human stabilin-1 (Supplementary Figure 1 is available online at http://www.nature.com/cdd/index.html).
Transfection
The complete stabilin-2 cDNA was amplified from human spleen cDNA (Invitrogen) and cloned into pcDNA3.1(−)/Myc-His vector (Invitrogen). Mouse fibroblast L cells were transfected with human stabilin-2 cDNA using Lipofectamine (Invitrogen). For stable transfection, L cells were selected in G418 (400 μg/ml). Individual G418-resistant colonies were isolated after 10–12 days of culture. The final clones were designated L/Stab-2-#. Negative control clones were selected randomly from transfection with empty vector (L/Mock). The expression of stabilin-2 was assessed via Western blotting and flow cytometry.
Fluorescence-activated cell sorting analysis
For the analysis of surface staining, the cells were incubated with 1 μg/ml of mAb 5G3 on ice for 30 min with gentle agitation every 5 min. The cells were washed twice, resuspended in PBS, and incubated with 4 μg/ml of FITC-conjugated goat anti-mouse IgG antibody for 45 min on ice. After three washes in PBS, the cells were resuspended in PBS, and analyzed via flow cytometry on a FACScalibur cytometer (BD Biosciences).
Liposome preparation and liposome incorporation assay
Liposomes containing the indicated phospholipid and PC at a molar ratio of 50 : 50 were prepared. The lipids were mixed in chloroform and dried under nitrogen gas. The dried lipids were then resuspended in PBS at a final concentration of 5 mM. The mixture was mixed, and then sonicated for 10 min on ice. Fluorescence-labelled liposomes were prepared in the presence of N-(lissamin rhodamine B sulfonyl)-L-α-phosphatidylethanolamine (Avanti Polar Lipids) at 1% of total phospholipids, as described previously.38 The affinity of fluorescent liposomes to L cell transfectants was quantified with a fluorescent microplate reader (Biolumin 960, Molecular Dynamics) set to an excitation wavelength of 549 nm and an emission wavelength of 565 nm.
Reconstitution of plasma membrane outer leaflet with PS
The association of liposomes containing PS with Jurkat T cells was conducted as previously described39 with slight modifications. In brief, the cells were collected, washed twice, and treated with 10 μM N-ethylmaleimide (Sigma) for 5 min at 37°C in order to inhibit aminophospholipid translocase (APT) activity. The cells were then exposed to varying quantities of liposomes for 30 min at 37°C, washed twice in PBS, and used immediately thereafter. Surface PS was verified via evaluation of annexin V-FITC binding ability, as determined via flow cytometry. In some cases, the added PS was removed by washing the cells three times in PBS containing 2% bovine serum albumin, and the cells were incubated for 6 h in tissue culture medium at 37°C in order to allow for the translocation of remaining PS to the inner leaflet via APT. PS removal was also verified via flow cytometry, as described above.
Binding and phagocytosis of aged RBCs
Aged RBCs were prepared via the incubation of cells in PBS (20% hematocrit) at 37°C, as described previously.10 Exposure of PS on the surface of aged RBCs was detected via annexin V-FITC using an annexin V Apoptosis detection kit (Santa Cruz). Aged RBCs were added to the L cell transfectants or HMDMs, and incubated for 1 h to assess binding in the presence or absence of mAb (5G3) (20 μg/ml), isotype-matched control Ab (IgG1, 20 μg/ml), or 10 μM liposome containing PC, or 50 : 50 molar ratios of PS : PC, PI : PC, PG : PC, PE : PC, PA : PC, or PG : PC. After washing away the unbound RBCs, the uningested RBCs were lysed via the addition of deionized H2O for 10 s, followed by immediate replacement with DMEM as described.13 The cells were fixed with methanol and stained with a Diff Quick staining kit (IMEB Inc.). The binding and phagocytosis of aged RBCs were quantified via light microscopy before and following hypotonic lysis, respectively. The percentages of binding and phagocytosis were determined as the percentages of phagocytes positive for binding and engulfment, respectively. At least 100 cells were scored per well, and the experiments were repeated at least three times.
In certain experiments, the phagocytosis assay was conducted in the presence of monoclonal anti-stabilin-2 antibody. L cell transfectants or HMDMs were preincubated with anti-stabilin-2 antibody for 1 h at 37°C before the addition of aged RBCs.
Phagocytosis of apoptotic cells
Apoptotic cells were added to the L cell transfectants or HMDMs, and incubated for 1 h to evaluate uptake in the presence of mAb 5G3 (20 μg/ml), isotype-matched control Ab (IgG1, 20 μg/ml), or 10 μM liposome containing PS or PC. After extensive washing away the uningested cells, the cells were stained using a Diff Quick staining kit, and the uptake of apoptotic cells was quantified under a light microscope. Under a light microscope, the engulfed apoptotic cells were lightly stained, and the nuclei of engulfing cells were dislocated by the engulfed apoptotic cells. Cells harboring lightly stained apoptotic cells and manifesting nuclear dislocation by apoptotic cells were scored as engulfing cells. This convention may have underestimated the number of engulfing cells. The percentage of phagocytosis was determined as the percentage of phagocytes positive for engulfment.
In the phagocytosis assay using HMDMs, apoptotic Jurkat T cells were labeled with PC-NBD and added to HMDMs. After 1 h of incubation, the uningested apoptotic cells were removed by extensive washing. To further identify the engulfed cells, fluorescence derived from bound apoptotic cells (remnant cells) was quenched using the trypan blue described previously.40 HMDMs engulfing fluorescent-labelled apoptotic cells were quantified under confocal microscopy.
Time-lapse video microscopy
L/Stab-2 cells were seeded into collagen type I coated dishes (IWAKI, Asahi techno glass). The culture dishes were placed in a chamber on the microscope (Leica) at 37°C in an atmosphere of 5% CO2. DIC (differential interference contrast) images were acquired using a × 100 objective and a cooled CCD camera (Cool Snap HQ, Photometrics). Images were controlled using Metamorph image processing software (Universal Imaging Corp.). Two hours after the addition of aged RBCs or apoptotic cells, we began to collect images every 2–20 s for 2 h.
Lentivirus-mediated shRNA production
Three 20-nt shRNA sequences, shRNA1: 5′-CAAACTGGAATGCAAATGCC-3′, shRNA2: 5′-TGGCAAGGAAGGCTGACCTC-3′, and shRNA3: 5′-CGCAGCAGTGGAATTGTCAT-3′, corresponding to bases 840–959, 1324–1343, and 3174–3193, respectively, in the stabilin-2 mRNA sequence (accession number: NM_017564) were designed. Candidate oligonucleotides were synthesized and cloned into the pSuper/basic vector (OligoEngine). pSuper/shRNA2 significantly downregulated stabilin-2 protein expression in the CHO-K1 cells co-transfected with a vector expressing stabilin-2, and the expression cassette of pSuper/shRNA2 was subcloned into the pRNAT-U6.1/Lenti lentiviral vector (GenScript). Scrambled shRNA (shCont) was employed as a control for stabilin-2 shRNA (shStab-2). All constructs were confirmed via sequence analysis. Lentiviral transduction was conducted in accordance with the manufacturer's instructions. In brief, the pRNAT-shStab-2 vector was cotransfected with the packaging vector (Invitrogen) into 293FT cells (Invitrogen). The supernatant was collected after 72 h, and filtered through a 0.22 μm pore acetate filter. HMDMs were seeded at 2 × 105 cell/ml into 24-well plates. Lentiviral particles and 8 μg/ml of polybrene (Sigma) were added to the culture. In our system, using lentivirus-mediated-shRNA (shStab-2), the depletion of stabilin-2 via shRNA-mediated gene silencing was determined to be effective 3 days after infection. pRNAT-shStab-2 and pRNAT-shCont vectors also harbored the transgene for GFP, allowing for the monitoring of infection efficiency.
RNA isolation, reverse transcription and PCR
Total RNA was isolated from spleen tissues, as well as several primary macrophages and macrophage cell lines, using standard Trizol RNA isolation protocol (Invitrogen). Reverse transcription was performed with M-MLV reverse transcriptase (Promega) using 2 μg of total RNA (Invitrogen) for 50 min at 42°C, followed by 3 min at 95°C. The human stabilin-2 was amplified in a Perkin-Elmer 2400 PCR thermocycler under the following conditions: 94°C for 2 min (initial denaturation); 94°C for 30 s, 58°C for 30 s, 72°C for 45 s (35 cycles); and 72°C for 10 min (final extension). The primers utilized in this study are shown in Supplementary Table 1.
TGF-β assays
HMDMs were treated with either aged RBCs, 10 μM liposomes containing 50 molar% PS or PC, mAb 5G3 (20 μg/ml), or isotype-matched control Ab (20 μg/ml). The supernatants were evaluated for cytokine production via ELISA 18 h later (R&D system). L cell transfectants were treated with mAb 5G3 or isotype-matched control Ab and assessed for TGF-β production 18 h later.
Statistical analysis
Statistical significance was assessed via ANOVA. A P-value of <0.05 was considered to be statistically significant.
Accession codes
Abbreviations
- PS:
-
phosphatidylserine
- RBCs:
-
red blood cells
- TGF-β:
-
transforming growth factor-β
- PSR:
-
phosphatidylserine receptor
- LDL:
-
low-density lipoprotein
- PC:
-
phosphatidylcholine
- NBD:
-
7-nitrobenz-2-oxa-1,3-diazole
- HMDMs:
-
human monocyte-derived macrophages
- mAb:
-
monoclonal antibody
- shRNA:
-
short hairpin RNA
- PMA:
-
phorbol myristate acetate
- FITC:
-
fluorecein-5-isothiocyanate.
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Acknowledgements
This work was supported by a National Research Laboratory Program (M10104000036-01J0000-01610); by the Grant No. RTI04-01-01 from the Regional Technology Innovation Program of the MOCIE, Advanced Medical Technology Cluster for Diagnosis and Prediction at Kyungpook National University from MOCIE; and by the Brain Korea 21 Project, in 2007.
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Supplementary Information accompanies the paper on Cell Death and Differentiation website (http://www.nature.com/cdd)
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Park, SY., Jung, MY., Kim, HJ. et al. Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor. Cell Death Differ 15, 192–201 (2008). https://doi.org/10.1038/sj.cdd.4402242
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DOI: https://doi.org/10.1038/sj.cdd.4402242
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