Abstract
OBJECTIVE: The gene that codes for a novel uncoupling protein, UCP2, has been linked to obesity in animal models. Markers encompassing the UCP2 locus have been linked to energy expenditure in humans. We studied the role of a common amino acid substitution, replacing an alanine (A) with a valine (V) at codon 55, of the coding region of the UCP2 gene for 24‐h energy expenditure and respiratory quotient (RQ) in healthy subjects
METHODS:24‐h energy expenditure and RQ were measured in calorimeters in 60 healthy subjects. The UCP2 polymorphism was determined by restriction fragment length polymorphism-generating polymerase chain reaction.
RESULTS: Age, gender and body fat were not different between groups, the number of subjects in each groups was A/A: 35% (n=21), A/V: 48% (n=29), and V/V: 17% (n=10). Twenty‐four‐hour energy expenditure, adjusted for fat-free mass, fat mass, and spontaneous physical activity, was 311 kJ/d lower (95% confidence interval: 24–598 kJ/d, P=0.03) in the V/V homozygotes than in the A/A and A/V genotypes. The V/V had ∼20% higher 24‐h spontaneous physical activity, particularly higher at night (P<0.005). Energy expenditure due to higher spontaneous physical activity counteracted the V/V group’s lower 24‐h resting energy expenditure for a given body size and composition. 24‐h RQ adjusted for energy balance, age, sex and spontaneous physical activity, was higher in the V/V homozygotes than in the AA and A /V groups (P<0.05).
CONCLUSIONS:Subjects with the V/V genotype of the UCP2 gene exhibit an enhanced metabolic efficiency and lower fat oxidation than the A/A and A/V genotypes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Astrup, A., Toubro, S., Dalgaard, L. et al. Impact of the v/v 55 polymorphism of the uncoupling protein 2 gene on 24‐h energy expenditure and substrate oxidation. Int J Obes 23, 1030–1034 (1999). https://doi.org/10.1038/sj.ijo.0801040
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.ijo.0801040
Keywords
This article is cited by
-
Interactions between UCP2 SNPs and telomere length exist in the absence of diabetes or pre-diabetes
Scientific Reports (2016)
-
Association between UCP2A55V polymorphism and risk of cardiovascular events in patients with multi-vessel coronary arterial disease
BMC Medical Genetics (2013)
-
Uncoupling protein 2 expression affects androgen synthesis in polycystic ovary syndrome
Endocrine (2013)
-
Uncoupling protein 2 gene polymorphisms are associated with obesity
Cardiovascular Diabetology (2012)
-
A/ASP/VAL allele combination of IGF1R, IRS2, and UCP2 genes is associated with better metabolic profile, preserved energy expenditure parameters, and low mortality rate in longevity
AGE (2012)