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Insulin resistance but not visceral adipose tissue is associated with plasminogen activator inhibitor type 1 levels in overweight and obese premenopausal African-American women

International Journal of Obesity volume 27, pages 82–87 (2003)Cite this article

Abstract

OBJECTIVE: To compare plasma plasminogen activator inhibitor type 1 (PAI-1) levels and to examine the association of PAI-1 with visceral adiposity and other components of the metabolic syndrome in overweight and obese premenopausal African-American (AA) and Caucasian (CC) women.

DESIGN: Cross-sectional study.

SUBJECTS: 33 CC and 23 AA healthy, overweight and obese, premenopausal women (age 19–53 y, body mass index 28.1–48.9 kg/m2).

MEASUREMENTS: Body mass index, sagittal diameter, waist circumference, percentage body fat, visceral and subcutaneous adipose tissue (by anthropometry, magnetic resonance imaging (MRI), and bioelectric impedance techniques), PAI-1, leptin, lipids, glucose, insulin, and insulin resistance (by HOMA IR).

RESULTS: AA women had lower triglyceride levels and less visceral adipose tissue (VAT) volume than CC despite similar BMI. PAI-1 levels were not significantly different in the two groups. Insulin resistance was associated with PAI-1 in both groups but only in CC women were VAT, triglyceride, HDL cholesterol and blood pressure related to plasma PAI-1 levels. Multiple regression analysis showed that VAT in CC and insulin resistance in AA were independent predictors of PAI-1.

CONCLUSION: VAT is significantly associated with circulating PAI-1 levels in overweight and obese CC but not AA premenopausal women.

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References

  1. Larsson B . Obesity, fat distribution and cardiovascular disease. Int J Obes 1991; 15: 53–57.

    PubMed  Google Scholar 

  2. Bjorntorp P . Abdominal fat distribution and disease: an overview of epidemiological data. Ann Med 1992; 24: 15–18.

    Article  CAS  Google Scholar 

  3. Reaven GM . Pathogenesis of insulin resistance in human and disease. Physiol Rev 1995; 75: 473–486.

    Article  CAS  Google Scholar 

  4. Juhan-Vague I & Alessi MC . PAI-1, obesity, insulin resistance and risk of cardiovascular events. Thromb Haemost 1997; 78: 656–660.

    Article  CAS  Google Scholar 

  5. Juhan-Vague I, Alessi MC & Vague P . Increased plasma plaminogen activator inhibitor-1 levels: a possible link between insulin resistance and atherothrombosis. Diabetologia 1991; 34: 457–462.

    Article  CAS  Google Scholar 

  6. Lindahl B, Asplund K, Eliasson M & Evrin PE . Insulin resistance syndrome and fibrinolytic activity: the northern Sweden MONICA study. Int J Epidemiol 1996; 25: 291–299.

    Article  CAS  Google Scholar 

  7. Festa A, D'Agostino R Jr, Mykkanen L, Tracy RP, Zaccaro DJ, Hales CN & Haffner SM . Relative contribution of insulin and its precursors to fibrinogen and PAI-1 in a large population with different states of glucose tolerance. The Insulin Resistance Atherosclerosis Study (IRAS). Arterioscler Thromb Vasc Biol 1999; 19: 562–568.

    Article  CAS  Google Scholar 

  8. Meigs JB, Mittleman MA, Nathan DM, Tofler GH, Singer DE, Murphy-Sheehy PM, Lipinska I, D'Agostino RB & Wilson PW . Hyperinsulinemia, hyperglycemia and impaired hemostasis: the Framingham Offspring Study. JAMA 2000; 283: 221–228.

    Article  CAS  Google Scholar 

  9. Vague P, Juhan-Vague I, Aillaud MF, Badier C, Viard R, Alessi MC & Collen D . Correlation between blood fibrinolytic activity, plasminogen activator inhibitor level, plasma insulin level, and relative body weight in normal and obese subjects. Metabolism 1986; 35: 250–253.

    Article  CAS  Google Scholar 

  10. Vague P, Juhan-Vague I, Chabert V, Allesi MC & Atlan C . Fat distribution and plasminogen activator inhibitor activity in nondiabetic obese women. Metabolism 1989; 38: 913–915.

    Article  CAS  Google Scholar 

  11. Janand-Delenne B, Chagnaud C, Raccah D, Alessi MC, Juhan-Vague I & Vague P . Visceral fat as a main determinant of plasminogen activator inhibitor 1 level in women. Int J Obes Relat Metab Disord 1998; 22: 312–317.

    Article  CAS  Google Scholar 

  12. Giltay EJ, Elbers JMH, Gooren LJG, Emeis JJ, Kooistra T, Asscheman H & Stehouwer CD . Visceral fat accumulation is an important determinant of PAI-1 levels in young, non obese men and women. Modulation by cross-sex hormone administration. Arterioscler Thromb Vasc Biol 1998; 18: 1716–1722.

    Article  CAS  Google Scholar 

  13. Mussoni L, Mannuci L, Sirtori M, Camera M, Maderna P, Sironi L & Tremoli E . Hypertriglyceridemia and regulation of fibrinolytic activity. Arterioscler Thromb 1992; 12: 19–27.

    Article  CAS  Google Scholar 

  14. Asplund-Carlson A, Hamsten A, Wiman B & Carlson LA . Relationship between plasma plasminogen activator inhibitor-1 activity and VLDL triglyceride concentration, insulin levels and insulin sensitivity: studies in randomly selected normo- and hyper-triglyceridaemic men. Diabetologia 1993; 36: 817–825.

    Article  CAS  Google Scholar 

  15. Shimomura I, Funahashi T, Takahashi M, Maeda K, Kotani K, Nakamura T, Yamashita S, Miura M, Fukuda Y, Takemura K, Tokunaga K & Matsuzawa Y . Enhanced expression of PAI-1 in visceral fat: possible contributor to vascular disease in obesity. Nat Med 1996; 2: 800–803.

    Article  CAS  Google Scholar 

  16. Crandall DL, Quinet EM, Morgan GA, Busler DE, McHendry-Rinde B & Kral JG . Synthesis and secretion of plasminogen activator inhibitor-1 by human preadipocytes. J Clin Endorinol Metab 1999; 84: 3222–3227.

    Article  CAS  Google Scholar 

  17. Erkisson P, Reynisdottir S, Lonnqvist F, Stemme V, Hamsten A & Arner P . Adipose tissue secretion of plasminogen activator inhibitor-1 in non-obese and obese individuals. Diabetologia 1998; 41: 65–71.

    Article  Google Scholar 

  18. Alessi MC, Peiretti F, Morangue P, Henry M, Nalbone G & Juhan-Vague I . Production of plasminogen activator inhibitor 1 by human adipose tissue. Possible link between visceral fat accumulation and vascular disease. Diabetes 1997; 46: 860–867.

    Article  CAS  Google Scholar 

  19. Kuczmarski RJ, Flegal KM, Campbell SM & Johnson CL . Increasing prevalence of overweight among US adults: the National Health and Nutritional Examination Surverys, 1960–1991. JAMA 1994; 272: 205–211.

    Article  CAS  Google Scholar 

  20. Kumanyika SK . Obesity in black women. Epidemiol Rev 1987; 9: 31–50.

    Article  CAS  Google Scholar 

  21. Adams-Campbell LL, Nwankwo M, Omene J, Haile GT & Kuller LH . Body fat distribution patterns and blood pressure in black and white women. J Natl Med Assoc 1990; 82: 573–576.

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Wing RR, Kuller LH, Bunker C, Matthews K, Caggiula A, Meihlan E & Kelsey S . Obesity, obesity-related behaviors, and coronary heart disease risk factors in black and white premenopausal women. Int J Obes 1989; 13: 511–519.

    CAS  PubMed  Google Scholar 

  23. Zillikens MC & Conway JM . Anthropometry in blacks: applicability of generalized skinfold equations and differences in fat patterning between black and whites. Am J Clin Nutr 1990; 52: 45–51.

    Article  CAS  Google Scholar 

  24. Perry AC, Applegate EB, Jackson ML, Deprima S, Goldberg RB, Ross R, Kempner L & Feldman BB . Racial differences in visceral adipose tissue but not anthropometric markers of health-related variables. J Appl Physiol 2000; 89: 636–643.

    Article  CAS  Google Scholar 

  25. Conway JM, Yanovski SZ, Avila NA & Hubbard VS . Visceral adipose tissue differences in black and white women. Am J Clin Nutr 1995; 61: 765–771.

    Article  CAS  Google Scholar 

  26. Lovejoy JC, de la Bretonee JA, Klemperer M & Tulley R . Abdominal fat distribution and metabolic risk factors: effects of race. Metabolism 1996; 45: 1119–1124.

    Article  CAS  Google Scholar 

  27. Karter AJ, Mayer-Davis EJ, Selby JV, D'Agostino RB Jr, Haffner SM, Sholinsky P, Bergman R, Saad MF & Hamman RF . Insulin sensitivity and abdominal obesity in African-American, Hispanic, and non-Hispanic white men and women. The Insulin Resistance and Atherosclerosis Study. Diabetes 1996; 45: 1547–1555.

    Article  CAS  Google Scholar 

  28. Dowling HJ & Pi-Sunyer X . Race-dependent health risks of upper body obesity. Diabetes 1993; 42: 537–543.

    Article  CAS  Google Scholar 

  29. Albu JB, Murphy L, Frager DH, Johnson JA & Pi-Sunyer FX . Visceral fat and race-dependent health risks in obese nondiabetic premenopausal women. Diabetes 1997; 46: 456–462.

    Article  CAS  Google Scholar 

  30. Segal KR, Van Loan M, Fitzgerald PI, Hodgdon JA & Van Itallie TB . Lean body mass estimation by bioelectrical impedance analysis: a four-site cross-validation study. Am J Clin Nutr 1988; 47: 7–14.

    Article  CAS  Google Scholar 

  31. Goran MI & Khaled MA . Cross-validation of fat-free mass estimated from body density against bioelectrical resistance: effects of obesity and gender. Obes Res 1995; 3: 531–539.

    Article  CAS  Google Scholar 

  32. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF & Turner RL . Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28: 412–419.

    Article  CAS  Google Scholar 

  33. Friedewald WT, Levy RS & Fredrickson DS . estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499–502.

    CAS  Google Scholar 

  34. Ross R, Leger L, Morris D, de Guise J & Guardo R . Quantification of adipose tissue by MRI: relationship to antthropometric variables. J Appl Physiol 1992; 72: 787–795.

    Article  CAS  Google Scholar 

  35. Kooistra T, Bosma PJ, Tons HAM, van den Berg AP, Meyer P & Princen HMG . Plasminogen activator inhibitor 1: biosynthesis and mRNA level are increased by insulin in cultured human hepatocytes. Thromb Haemost 1989; 62: 723–728.

    CAS  PubMed  Google Scholar 

  36. Schneider DJ, Absher PM & Ricci MA . Dependance of augmentation of arterial endothelial cell expression of plasminogen activator inhibitor type 1 by insulin on soluble factors released from vascular smooth muscle cells. Circulation 1997; 96: 2868–2876.

    Article  CAS  Google Scholar 

  37. Potter Van Loon BJ, de Bart ACW, Radder JK, Frouch M, Kluft C & Menders AE . Acute exogenous hyperinsulinemia does not result in elevation of plasma plasminogen activator inhibitor 1 (PAI-1) in human. Fibrinolysis 1990; 4: 93–94.

    Article  Google Scholar 

  38. Vuorinen-Markkola H, Puhakainen I & Yki-Jarvinen H . No evidence for short-term regulation of plasminogen activator inhibitor in man. Thromb Haemost 1992; 67: 117–120.

    Article  CAS  Google Scholar 

  39. Landin K, Tengborn L, Chmielewska J, von Schenk H & Smith U . The acute effect of insulin on tissue plasminogen activator inhibitor in man. Thromb Haemost 1991; 65: 130–133.

    Article  CAS  Google Scholar 

  40. Eriksson P, Van Harmelen V, Hoffsteadt J, Lundquist P, Vidal H, Stemme V, Hamsten A, Arner P & Reynisdottir S . Regional Variation in plasminogen activator inhibitor-1 expression in adipose tissue from obese individuals. Thromb Haemost 2000; 83: 545–548.

    Article  CAS  Google Scholar 

  41. Hill JO, Sidney S, Lewis CE, Tolan K, Scherzinger AL & Stamm ER . Racial differences in amounts of visceral adipose tissue in young adults: the CARDIA (Coronary Artery Risk Development in Young Adults) study. Am J Clin Nutr 1999; 69: 381–387.

    Article  CAS  Google Scholar 

  42. Blair D, Habicht J-P, Sims EAH, Sylwester D & Abraham S . Evidence for an increased risk of hypertension with centrally located body fat and the effect of sex and race on this risk. Am J Epidemiol 1984; 119: 526–540.

    Article  CAS  Google Scholar 

  43. Stevens J, Keil JE, Rust PF, Tyroler HA, Davis CE & Gazes PC . Body mass index and body girths as predictors of mortality in black and white women. Arch Intern Med 1992; 152: 1257–1262.

    Article  CAS  Google Scholar 

  44. Juhan-Vague I, Pyke SDM, Alessi MC, Jespersen J, Haverkate F & Thompson SG . on behalf of the ECAT Study Group. Fibrinolytic factors and the risk of myocardial infarction or sudden death in patients with angina pectoris. Circulation 1996; 94: 2057–2063.

    Article  CAS  Google Scholar 

  45. Cigolini M, Targher G, Seidell JC, Tonoli M, Schiavon R, Agostino G & De Sandre G . Relationships of blood pressure to fibrinolysis: influence of anthropometry, metabolic profile and behavioral variables. J Hypertens 1995; 3: 659–666.

    Article  Google Scholar 

  46. Jeng JW, Sheu HH, Jeng C, Huang S & Shieh S . Impaired fibrinolysis and insulin resistance in hypertension. Am J Hypertens 1996; 26: 484–490.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, University of Miami School of Medicine, Miami, Florida, USA

    M P Solano & R B Goldberg

  2. Human Performance Laboratory, University of Miami, Miami, Florida, USA

    A C Perry & X Wang

  3. School of Physical and Health Education, Queens University, Kinston, Ontario, Canada

    R Ross

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Solano, M., Perry, A., Wang, X. et al. Insulin resistance but not visceral adipose tissue is associated with plasminogen activator inhibitor type 1 levels in overweight and obese premenopausal African-American women. Int J Obes 27, 82–87 (2003). https://doi.org/10.1038/sj.ijo.0802192

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