Abstract
Objective:
The purpose of this study was to determine whether common single nucleotide polymorphisms (SNPs) at the adiponectin (ADIPOQ) locus influence changes in circulating adiponectin and the features of insulin resistance in response to a weight loss intervention.
Subjects:
In total, 294 nondiabetic/overweight–obese Koreans participated in a clinical intervention study lasting 12 weeks involving a caloric reduction of −300kcal/day.
Methods:
Plasma adiponectin, blood lipids, glucose and insulin concentrations were measured at baseline and after weight loss. Insulin resistance was estimated by homeostasis model assessment insulin resistance (HOMA-IR) derived from fasting glucose and insulin concentrations. We genotyped for three SNPs, 45T>G, 276G>T and −11377C>G.
Results:
At baseline, HOMA-IR was significantly higher in GG homozygotes than in carriers of the T allele at SNP276G>T of the adiponectin gene (P<0.05). With regard to SNP45T>G and SNP −11377C>G, we did not find any genotype related differences in baseline levels of HOMA-IR and adiponectin. In the 45/276 haplotype test, homozygous for the TG haplotype had significantly lower concentrations of plasma adiponectin (P<0.05). After the 12-week weight loss intervention, the significant decreases in HOMA-IR (P<0.001) and increases in adiponectin (P<0.01) were observed in GG homozygotes at SNP276, which were not shown in carriers of the T allele. Furthermore, there was a significant difference in the decreases in HOMA-IR between the GG homozygotes and carriers of the T allele at SNP276 (P<0.05). Regarding SNP45T>G and SNP −11377C>G, there was no association between SNP45T>G and SNP −11377C>G and decreases in HOMA-IR. In the 45/276 haplotype test, there was a significant difference in changes of adiponectin levels among those with different haplotype combinations (P<0.05).
Conclusion:
The SNP276G>T of the ADIPOQ gene is associated with different responses of circulating adiponectin and insulin resistance to mild weight loss in overweight-obese subjects.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Nutrition Survey Health Examination. Ministry of Health and Welfare. Nutrition Survey Health Examination: Seoul, South Korea, 2002.
Klein S . Outcome success in obesity. Obes Res 2001; 9: 354S–358S.
Davidson MH, Hauptman J, DiGirolamo M, Foreyt JP, Halsted CH, Heber D et al. Weight control and risk factor reduction in obese subjects treated for 2 years with orlistat. JAMA 1999; 281: 235–242.
Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley RE et al. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab 2001; 86: 1930–1935.
Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y et al. Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol 2000; 20: 1595–1599.
Kazumi T, Kawaguchi A, Sakai K, Hirano T, Yoshino G . Young men with high-normal blood pressure have lower serum adiponectin, smaller LDL size, and higher elevated heart rate than those with optimal blood pressure. Diabetes Care 2002; 25: 971–976.
Matsubara M, Maruoka S, Katayose S . Decreased plasma adiponectin concentrations in women with dyslipidemia. J Clin Endocrinol Metab 2002; 87: 2764–2769.
Yang WS, Lee WJ, Funahashi T, Tanaka S, Matsuzawa Y, Chao CL et al. Weight reduction increases plasma levels of an adipose-derived anti-inflammatory protein, adiponectin. J Clin Endocrinol Metab 2001; 86: 3815–3819.
Fruebis J, Tsao TS, Javorschi S . Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice. Proc Natl Acad Sci USA 2001; 98: 2005–2010.
Garaulet M, Viguerie N, Porubsky S, Klimcakova E, Clement K, Langin D et al. Adiponectin gene expression and plasma values in obese women during very-low-calorie diet. Relationship with cardiovascular risk factors and insulin resistance. J Clin Endocrinol Metab 2001; 89: 756–760.
Vasseur F, Helbecque N, Dina C, Lobbens S, Delannoy V, Gaget S et al. Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians. Hum Mol Genet 2002; 11: 2607–2614.
Hara K, Boutin P, Mori Y, Tobe K, Dina C, Yasuda K et al. Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population. Diabetes 2002; 52: 536–540.
Menzaghi C, Ercolino T, Di Paola R, Berg AH, Warram JH, Scherer PE et al. A haplotype at the adiponectin locus is associated with obesity and other features of the insulin resistance syndrome. Diabetes 2002; 51: 2306–2312.
Chandran M, Phillips SA, Ciaraldi T, Henry RR . Adiponectin: more than just another fat cell hormone? Diabetes Care 2003; 26: 2442–2450.
Steering Committee. Assessment diagnosis. The Asia-Pacific perspective: redefining obesity and its treatment, 2002. pp 15–21.
Mathews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC . Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28: 412–419.
Case CC, Jones PH, Nelson K, O'Brian SE, Ballantyne CM . Impact of weight loss on the metabolic syndrome. Diabetes Obes Metab 2002; 4: 407–414.
Haluzik M, Parizkova J, Haluzik MM . Adiponectin and its role in the obesity-induced insulin resistance and related complications. Physiol Res 2004; 53: 123–129.
Faraj M, Havel PJ, Phelis S, Blank D, Sniderman AD, Cianflone K . Plasma acylation-stimulating protein, adiponectin, leptin, and ghrelin before and after weight loss induced by gastric bypass surgery in morbidly obese subjects. J Clin Endocrinol Metab 2003; 88: 1594–1602.
Hulver MW, Zheng D, Tanner CJ, Houmard JA, Kraus WE, Slentz CA et al. Adiponectin is not altered with exercise training despite enhanced insulin action. Am J Physiol Endocrinol Metab 2002; 283: E861–E865.
Thamer C, Haap M, Bachmann O, Zur Nieden T, Tschritter O, Stefan N et al. Serum adiponectin levels predict the effect of short-term dietary interventions on insulin sensitivity in humans. Diabetologia 2004; 47: 1303–1305.
Stumvoll M, Tschritter O, Fritsche A . Association of the T-G polymorphism in adiponectin (exon 2) with obesity and insulin sensitivity: interaction with family history of type 2 diabetes. Diabetes 2002; 51: 37–41.
Bacci S, Menzaghi C, Ercolino T, Ma X, Rauseo A, Salvemini L et al. The +276 G/T single nucleotide polymorphism of the adiponectin gene is associated with coronary artery disease in type 2 diabetic patients. Diabetes Care 2004; 27: 2015–2020.
Qi L, Li T, Rimm E, Zhang C, Rifai N, Hunter D et al. The +276 Polymorphism of the APM1 gene, plasma adiponectin concentration, and cardiovascular risk in diabetic men. Diabetes 2005; 54: 1607–1610.
Gu HF, Abulaiti A, Ostenson CG, Humphreys K, Wahlestedt C, Brookes AJ et al. Single nucleotide polymorphisms in the proximal promoter region of the adiponectin (APM1) gene are associated with type 2 diabetes in Swedish caucasians. Diabetes 2004; 53: S31–S35.
Jang Y, Lee JH, Chae JS, Kim OY, Koh SJ, Kim JY et al. Association of the +276G>T polymorphism of the adiponectin gene with cardiovascular disease risk factors in nondiabetic Koreans. Am J Clin Nutr 2005; 82: 760–767.
Acknowledgements
We thank Patty W Siri of Children's Hospital Oakland Research Institute (CHORI) for her expert help. Financial support: (1) National Research Laboratory project, Ministry of Science & Technology (2005-01572) (2) Korea Science and Engineering Foundation (R01-2003-0000-11709-0) (3) Korea Health 21 R&D Projects, Ministry of Health & Welfare (00-PJ3-PG6-GN-01-0001), Republic of Korea (4) NIH/NHLBI # HL54776, and contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research Service (5) Korea Health 21 R&D Projects, Ministry of Health & Welfare, Republic of Korea (02-PJ1-PG1-CH15-0001).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shin, MJ., Jang, Y., Koh, S. et al. The association of SNP276G>T at adiponectin gene with circulating adiponectin and insulin resistance in response to mild weight loss. Int J Obes 30, 1702–1708 (2006). https://doi.org/10.1038/sj.ijo.0803338
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.ijo.0803338
Keywords
This article is cited by
-
Heterozygosity for the rs696217 SNP in the Preproghrelin Gene Predicts Weight Loss After Bariatric Surgery in Severely Obese Individuals
Obesity Surgery (2017)
-
The Association of SNP276G>T at Adiponectin Gene with Insulin Resistance and Circulating Adiponectin in Morbid Obese Patients After a Biliopancreatic Diversion Surgery
Obesity Surgery (2017)
-
Meta-analysis of the association of ADIPOQ G276T polymorphism with insulin resistance and blood glucose
Endocrine (2014)
-
Three adiponectin rs1501299G/T, rs822395A/C, and rs822396A/G polymorphisms and risk of cancer development: a meta-analysis
Tumor Biology (2013)
-
SNP276G>T polymorphism in the adiponectin gene is associated with metabolic syndrome in patients with Type II diabetes mellitus in Korea
European Journal of Clinical Nutrition (2010)