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Transfer of genomics information to flow cytometry: expression of CD27 and CD44 discriminates subtypes of acute lymphoblastic leukemia

Leukemia volume 19, pages 876–878 (2005)Cite this article

TO THE EDITOR

Expression profiling studies have provided data on an unprecedented number of genes that are expressed in malignant cells.1, 2, 3, 4 The critical number of genes which can predict a particular genotype is a matter of discussion. Downing pointed out that as few as 20 genes may be necessary for an accurate prediction of subsets and prognosis; these genes should be specifically studied, perhaps by methods like reverse transcription polymerase chain reaction (RT-PCR) or flow cytometry (FC) (Downing in Carroll et al5). FC, which shows the expression of molecules in mutual context on individual cells, appears optimal for such analysis.

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Acknowledgements

We thank the cytometric technicians Jana Ridoskova, Klara Pospisilova and Lucie Gondorcinova, and the molecular genetic technicians Katka Muzikova and Leona Reznickova and Marketa Kalinova. MV highly appreciates the practical and theoretical experience in the laboratory of Z Trajanoski (Institute of Biomedical Engineering and Christian Doppler Laboratory for Genomics and Bioinformatics, Graz University of Technology, Austria), as well as the friendly atmosphere. The collaboration of all Czech Pediatric Hematology (CPH) centers (leaders: B Blazek (Ostrava), Z Cerna (Plzen), J Hak (Hradec Kralove), Y Jabali (Ceske Budejovice), V Mihal (Olomouc), D Prochazkova (Usti nad Labem), J Stary (Praha) and J Sterba (Brno)) is appreciated. The work was supported by Grants Nos. GA UK 80/2004, MSM0021620813, IGA MZ CR 7430-3 and 6929-3.

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Authors and Affiliations

  1. CLIP – Childhood Leukemia Investigation Prague, 2nd Medical School, Charles University, Prague, Czech Republic

    M Vaskova, E Mejstrikova, T Kalina, J Trka & O Hrusak

  2. Department of Immunology, 2nd Medical School, Charles University, Prague, Czech Republic

    M Vaskova, E Mejstrikova, T Kalina & O Hrusak

  3. Department of Probability and Mathematical Statistics, Faculty of Mathematics and Physics, Charles University, Prague, Czech Republic

    P Martinkova & M Omelka

  4. Pediatric Hematology and Oncology, 2nd Medical School, Charles University, Prague, Czech Republic

    J Trka & J Stary

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  1. M Vaskova
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Correspondence to O Hrusak.

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Vaskova, M., Mejstrikova, E., Kalina, T. et al. Transfer of genomics information to flow cytometry: expression of CD27 and CD44 discriminates subtypes of acute lymphoblastic leukemia. Leukemia 19, 876–878 (2005). https://doi.org/10.1038/sj.leu.2403706

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