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Inhibitory interaction of c-Myb and GATA-1 via transcriptional co-activator CBP

Oncogene volume 19, pages 134–140 (2000)Cite this article

Abstract

Gene targeting experiments have revealed that transcription factors such as c-Myb and GATA-1 play crucial roles during hematopoietic differentiation. c-Myb is necessary in the immature cells of almost every hematopoietic lineage and GATA-1 is essential for the development of the erythroid lineage. In addition, CREB-binding protein (CBP) acts as a transcriptional adapter for various transcription factors, including c-Myb and GATA-1. In this paper, we show that the transcription factors c-Myb and GATA-1 each inhibit the transcriptional activity of the other and that any possible bipartite complexes c-Myb, GATA-1, and CBP could be formed, but the tripartite complex was hardly formed. The exclusive binding of GATA-1 and c-Myb to CBP is probably the molecular basis for the mutual inhibition of their transcriptional activity. Our data suggest that cross-talk between these three factors might be important for hematopoietic differentiation and that CBP functions as a key molecule during the process.

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Acknowledgements

We thank Drs M Obinata, J Miyazaki, S Nagata, C Kanei-Ishii, T Yasukawa, J Tanikawa and T Nomura for gifting us various materials, and Drs T Habu, N Wakabayashi, N Kanno and K Isoda for valuable discussion. Dr Kelly McNagny kindly read this manuscript critically. This work was supported in part by grants from the Ministry of Education, Science, Sports and Culture, Japanese Society for Promotion of Sciences (JSPS-RFTF98L01101), the Osaka Foundation for Promotion of Clinical Immunology and the Cell Science Research Foundation.

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Authors and Affiliations

  1. Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Tomomi Takahashi & Toru Nakano

  2. Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan

    Naruyoshi Suwabe & Masayuki Yamamoto

  3. Laboratory of Molecular Genetics, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Ibaraki, 305-0074, Japan

    Ping Dai & Shunsuke Ishii

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  1. Tomomi Takahashi
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Takahashi, T., Suwabe, N., Dai, P. et al. Inhibitory interaction of c-Myb and GATA-1 via transcriptional co-activator CBP. Oncogene 19, 134–140 (2000). https://doi.org/10.1038/sj.onc.1203228

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