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Human wig-1, a p53 target gene that encodes a growth inhibitory zinc finger protein

Oncogene volume 20, pages 5466–5474 (2001)Cite this article

Abstract

We previously identified a novel p53-induced mouse gene, wig-1, that encodes a 290 amino acid zinc finger protein (Varmeh-Ziaie et al., 1997). Here we have identified and characterized the human homolog of mouse wig-1. The human wig-1 protein is 87% identical to the mouse protein and contains three zinc finger domains and a putative nuclear localization signal. Human wig-1 mRNA and protein is induced following activation of wild type p53 expression in our BL41-ts p53 Burkitt lymphoma cells. Wig-1 is also induced in MCF7 cells following treatment with the DNA-damaging agent mitomycin C. Northern blotting detected low levels of wig-1 mRNA in normal human tissues. Fluorescence in situ hybridization mapped wig-1 to human chromosome 3q26.3-27. FLAG-tagged human wig-1 localizes to the nucleus. Ectopic overexpression of human wig-1 inhibits tumor cell growth in a colony formation assay. These results suggest that human wig-1 has a role in the p53-dependent growth regulatory pathway.

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Acknowledgements

This work was supported by grants from the Swedish Cancer Society (Cancerfonden), Ã…ke Wibergs Stiftelse, and Karolinska Institute Funds. We also thank David Israeli and Moshe Oren, Weizmann Institute of Science, Rehovot, Israel, for communicating unpublished data.

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Author notes

  1. Wang Qian

    Present address: National Institute for Medical Research, Divison of Virology, The Ridgeway, Mill Hill, NW7 1AA, London, UK

Authors and Affiliations

  1. Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institute, SE-171 76 Stockholm, Sweden

    Fredrik Hellborg, Cristina Mendez-Vidal, Charlotte Asker, Margareta Wilhelm & Klas G Wiman

  2. Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Stockholm, SE-171 77, Sweden

    Maria Kost-Alimova, Stefan Imreh & Klas G Wiman

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  1. Fredrik Hellborg
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Correspondence to Klas G Wiman.

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Hellborg, F., Qian, W., Mendez-Vidal, C. et al. Human wig-1, a p53 target gene that encodes a growth inhibitory zinc finger protein. Oncogene 20, 5466–5474 (2001). https://doi.org/10.1038/sj.onc.1204722

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