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The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H

Oncogene volume 21, pages 5031–5037 (2002)Cite this article

Abstract

Cyclin dependent kinases are regulated by phosphorylation and dephosphorylation of the catalytic cdk subunits, by assembly with specific cyclins and by specific inhibitor molecules. Recently, it turned out that cyclins are also phosphoproteins, which means that they are also potential targets for a regulation by phosphorylation and dephosphorylation. Here, we show that cyclin H was phosphorylated by protein kinase CK2. Like most other CK2 substrates cyclin H was much better phosphorylated by the CK2 holoenzyme than by the α-subunit alone. By using point mutants derived from the cyclin H sequence we mapped the CK2 phosphorylation site at threonine 315 at the C-terminal end of cyclin H. Phosphorylation at this position had no influence on the assembly of the cyclin H/cdk7/Mat1 complex. However, phosphorylation at amino acid 315 of cyclin H turned out to be critical for a full cyclin H/cdk7/Mat1 kinase activity when the CTD peptide of RNA polymerase II or cdk2 was used as a substrate.

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Acknowledgements

We thank Wolfgang Nastainczyk for peptide synthesis, Jürgen Günther for expert technical assistance and Dr Claudia Götz and Dr Michael Faust for many helpful discussions. M Montenarh is supported by grant Mo309/11-3 from Deutsche Forschungsgemeinschaft and from Fonds der Chemischen Industrie.

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  1. Eberhard Schneider

    Present address: Phylos GmbH, Industrial Park Hoechst, Building G 830, Frankfurt, 65926, Germany

  2. Norbert Schuster

    Present address: Ludwig Institute for Cancer Research, Box 595, Uppsala, 75124, Sweden

Authors and Affiliations

  1. Medical Biochemistry and Molecular Biology, University of the Saarland, Building 44, Homburg, D-66424, Germany

    Eberhard Schneider, Sabine Kartarius, Norbert Schuster & Mathias Montenarh

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  1. Eberhard Schneider
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Correspondence to Mathias Montenarh.

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Schneider, E., Kartarius, S., Schuster, N. et al. The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H. Oncogene 21, 5031–5037 (2002). https://doi.org/10.1038/sj.onc.1205690

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