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The role of nucleophosmin in centrosome duplication

Oncogene volume 21, pages 6170–6174 (2002)Cite this article

Abstract

In higher animal cells, duplication of centrosomes is triggered by CDK2/cyclin E-mediated phosphorylation. Nucleophosmin (NPM)/B23, a multifunctional protein, has recently been identified as one of the substrates of CDK2/cyclin E in centrosome duplication. Centrosome-bound NPM/B23 dissociates from centrosome upon phosphorylation by CDK2/cyclin E, which in turn triggers initiation of centriole duplication. Duplicated centrosomes remain free of NPM/B23 till mitosis. When the nuclear membrane breaks down during mitosis, NPM/B23 re-localizes to centrosomes. Upon cytokinesis, each daughter cell receives one centrosome bound by NPM/B23, which again dissociates from the centrosome upon exposure to CDK2/cyclin E at mid–late G1 phase of the next cell cycle. Thus, NPM/B23 would constitute one of the licensing systems for centrosome duplication, ensuring the coordination of centrosome and DNA duplication, which limiting duplication once per cell cycle.

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Acknowledgements

I thank Drs Kenji Fukasawa and Pheruza Tarapore for helpful discussion regarding to improvement of NPM/B23 immunostaining, and providing the immunostaining image shown in Figure 1. This work was supported by grants-in-aid from the Ministry of Education, Science, Sports and Culture, Japan (13760221).

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  1. Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515, Japan

    Masaru Okuda

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  1. Masaru Okuda
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Correspondence to Masaru Okuda.

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Okuda, M. The role of nucleophosmin in centrosome duplication. Oncogene 21, 6170–6174 (2002). https://doi.org/10.1038/sj.onc.1205708

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