Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Oncogenomics
  • Published:

Cloning and molecular characterization of a novel gene strongly induced by the adenovirus E1A gene in rat thyroid cells

Oncogene volume 22, pages 1087–1097 (2003)Cite this article

Abstract

Expression of the adenovirus E1A gene in the rat thyroid differentiated cell line PC Cl 3 induces thyrotropin-independent cell growth and impairs differentiation. However, the malignant phenotype is achieved only when the PC E1A cells are infected with other murine retroviruses carrying the v-abl, v-raf or polyoma middle-T genes. To determine through which genes E1A affects thyroid cells, we differentially screened PC Cl 3 and PC E1A cells. Here we report a new gene, named CL2, that is upregulated in PC E1A cells. The CL2 transcript is 4.4 kb long and encodes a 949 amino-acid protein. Conceptual translation of the open reading frame showed one product with a signal peptide, multiple nuclear localization signals and three newly described domains. Furthermore, in vivo, this protein was located juxtanuclear, which is suggestive of Golgian localization, and also in cytoplasm and nucleus/nucleolus. Finally, CL2 gene expression was drastically downregulated in human thyroid neoplastic cell lines and tissues.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8

Similar content being viewed by others

References

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W and Lipman DJ . (1997). Nucleic Acids Res., 25, 3389–3402.

  • Antoine M, Reimers K, Dickson C and Kiefer P . (1997). J. Biol. Chem., 272, 29475–29481.

  • Bailey TL and Gribskov M . (1998). J Comput. Biol., 5, 211–221.

  • Baldin V, Roman AM, Bosc-Bierne I, Amalric F and Bouche G . (1990). EMBO J., 9, 1511–1517.

  • Basolo F, Giannini R, Toniolo A, Casalone R, Nikiforova M, Pacini F, Elisei R, Miccoli P, Berti P, Faviana P, Fiore L, Monaco C, Pierantoni GM, Fedele M, Nikiforov YE, Santoro M and Fusco A . (2002). Int. J. Cancer, 97, 608–614.

  • Berlingieri MT, Portella G, Grieco M, Santoro M and Fusco A . (1988). Mol. Cell Biol., 8, 2261–2266.

  • Berlingieri MT, Santoro M, Battaglia C, Grieco M and Fusco A . (1993). Oncogene, 8, 249–255.

  • Bouche G, Gas N, Prats H, Baldin V, Tauber JP, Teissie J and Amalric F . (1987). Proc. Natl. Acad. Sci. USA, 84, 6770–6774.

  • Chinnadurai G . (1992). Oncogene, 7, 1255–1258.

  • Cone RD, Grodzicker T and Jaramillo M . (1988). Mol. Cell. Biol., 8, 1036–1044.

  • Dry KL, Aldred MA, Edgar AJ, Brown J, Manson FD, Ho MF, Prosser J, Hardwick LJ, Lennon AA, Thomson K et al. (1995). Hum. Mol. Genet., 4, 2347–2353.

  • Estour B, Van Herle AJ, Juillard GJ, Totanes TL, Sparkes RS, Giuliano AE and Klandorf H . (1989) Virchows Arch. B Cell Pathol. Incl. Mol. Pathol., 57, 167–174.

  • Evan G and Littlewood T . (1998). Science, 281, 1317–1322.

  • Fabien N, Fusco A, Santoro M, Barbier Y, Dubois PM and Paulin C . (1994). Cancer, 73, 2206–2212.

  • Fagin JA, Matsuo K, Karmakar A, Chen DL, Tang SH and Koeffler HP . (1993). J. Clin. Invest., 91, 179–184.

  • Fearnhead HO, McCurrach ME, O'Neill J, Zhang K, Lowe SW and Lazebnik YA . (1997). Genes Dev., 11, 1266–1276.

  • Fiore L, Pollina LE, Fontanini G, Casalone R, Berlingieri MT, Giannini R, Pacini F, Miccoli P, Toniolo A, Fusco A and Basolo F . (1997). J. Clin. Endocrinol. Metab., 82, 4094–4100.

  • Fusco A, Berlingieri MT, Di Fiore PP, Portella G, Grieco M and Vecchio G . (1987). Mol. Cell. Biol., 7, 3365–3370.

  • Gallimore PH and Turnell AS . (2001). Oncogene, 20, 7824–7835.

  • Gluzman Y . (1981). Cell, 23, 175–182.

  • Henderson JE, Amizuka N, Warshawsky H, Biasotto D, Lanske BM, Goltzman D and Karaplis AC . (1995). Mol. Cell Biol., 15, 4064–4075.

  • Hill DJ, Logan A, Ong M, De Sousa D and Gonzalez AM . (1992). Growth Factors, 6, 277–294.

  • Imamura T, Engleka K, Zhan X, Tokita Y, Forough R, Roeder D, Jackson A, Maier JA, Hla T and Maciag T . (1990). Science, 249, 1567–1570.

  • Kurosawa H, Goi K, Inukai T, Inaba T, Chang KS, Shinjyo T, Rakestraw KM, Naeve CW and Look AT . (1999). Blood, 93, 321–332.

  • Lynch MJ and Trainer DL . (1989). Cancer Res., 49, 5429–5434.

  • Maher DW, Lee BA and Donoghue DJ . (1989). Mol. Cell. Biol., 9, 2251–2253.

  • Marais R, Light Y, Paterson HF, Mason CS and Marshall CJ . (1997). J. Biol. Chem., 272, 4378–4383.

  • Marshall CJ . (1994). Curr. Opin. Genet. Dev., 4, 82–89.

  • Melillo RM, Santoro M, Ong SH, Billaud M, Fusco A, Hadari YR, Schlessinger J and Lax I . (2001). Mol. Cell. Biol., 21, 4177–4187.

  • Moroianu J and Riordan JF . (1994). Proc. Natl. Acad. Sci. USA, 91, 1677–1681.

  • Nakai K and Kanehisa M . (1992). Genomics, 14, 897–911.

  • Nielsen H, Engelbrecht J, Brunak S and von Heijne G . (1997). Protein Eng., 10, 1–6.

  • Pan J, Nakanishi K, Yutsudo M, Inoue H, Li Q, Oka K, Yoshioka N and Hakura A . (1996). FEBS Lett., 383, 21–25.

  • Pang XP, Hershman JM, Chung M and Pekary AE . (1989). Endocrinology, 125, 1783–1788.

  • Paraskeva C and Gallimore PH . (1980). Int. J. Cancer, 25, 631–639.

  • Rossini M . (1983). Virology, 131, 49–58.

  • Ruley HE . (1983). Nature, 304, 602–606.

  • Sambrook J, Fritsch EF and Maniatis T . (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  • Santoro M, Melillo RM, Grieco M, Berlingieri MT, Vecchio G and Fusco A . (1993). Cell Growth Differ., 4, 77–84.

  • Stow ND . (1982). Nucleic Acids Res., 10, 5105–5119.

  • Tanaka J, Ogura T, Sato H and Hatano M . (1987). Virology, 161, 62–72.

  • Thompson JD, Higgins DG and Gibson TJ . (1994). Nucleic Acids Res., 22, 4673–4680.

  • Tokunaga K, Taniguchi H, Yoda K, Shimizu M and Sakiyama S . (1986). Nucleic Acids Res., 14, 2829.

  • White E . (1995). Current Topics in Microbiology and Immunology: The Molecular Repertoire of Adenovirus. Doerfler W (ed). Spinger-Verlag: Berlin, Heidelberg, pp. 33–58.

    Google Scholar 

  • White E . (2001). Oncogene, 20, 7836–7846.

  • Whyte P, Ruley HE and Harlow E . (1988). J. Virol., 62, 257–265.

  • Whyte P, Williamson NM and Harlow E . (1989). Cell, 56, 67–75.

  • Zeki K, Azuma H, Suzuki H, Morimoto I and Eto S . (1991). Acta Endocrinol., 124, 60–66.

Download references

Author information

Author notes

  1. Roberta Visconti and Filippo Schepis: Roberta Visconti and Filippo Schepis contributed equally to this work.

Authors and Affiliations

  1. Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, via Pansini 5, Naples, 80131, Italy

    Roberta Visconti, Francesca Carlomagno & Massimo Santoro

  2. Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro ‘Magna Graecia’, via Campanella 5, 88100, Catanzaro, Italy

    Filippo Schepis, Rodolfo Iuliano, Li Zhang, Caterina Battaglia, Maria Luisa Martelli & Francesco Trapasso

  3. Dipartimento di Biologia e Patologia Cellulare e Molecolare ‘L. Califano’, Università degli Studi di Napoli ‘Federico II’, via Pansini 5, Naples, 80131, Italy

    Giovanna Maria Pierantoni & Alfredo Fusco

Authors
  1. Roberta Visconti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Filippo Schepis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rodolfo Iuliano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Giovanna Maria Pierantoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Francesca Carlomagno
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Caterina Battaglia
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Maria Luisa Martelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Francesco Trapasso
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Massimo Santoro
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Alfredo Fusco
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alfredo Fusco.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Visconti, R., Schepis, F., Iuliano, R. et al. Cloning and molecular characterization of a novel gene strongly induced by the adenovirus E1A gene in rat thyroid cells. Oncogene 22, 1087–1097 (2003). https://doi.org/10.1038/sj.onc.1206194

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1206194

Keywords

This article is cited by

Search

Advanced search

Quick links