Abstract
p21WAF1/cip1 plays a critical role in regulating intestinal cell proliferation, maturation and tumorigenesis. Our previous work demonstrated that inactivation of a single p21 allele in Apc1638+/− mice was sufficient to accelerate Apc-initiated tumor formation, and that inactivation of only one p21 allele was also sufficient to abrogate duodenal tumor inhibition by sulindac, a nonsteroidal anti-inflammatory drug. To dissect the role of p21 in sulindac inhibition of intestinal tumor development in Apc1638+/− mice, we quantified p21 expression from Apc+/−, p21+/+, +/− or −/− mice fed sulindac. In Apc+/−, p21 wild-type mice fed the sulindac supplemental diet, both p21 mRNA and protein were significantly increased in the flat mucosa and tumors of the duodenum. However, p21 was not induced by sulindac in the duodenal flat mucosa and tumors of Apc+/− mice in which one or both p21 alleles had been inactivated. Further investigation revealed that the cytosine residues in a CpG cluster in the promoter region of the mouse p21 gene displayed hypermethylation in the Apc+/−, p21+/− mice. This suggested that although the p21+/− mice retained a wild-type allele, this allele was functionally modulated by hypermethylation, and that the inability of sulindac to inhibit tumor formation in Apc+/−, p21+/− mice is likely due to the inability to induce expression of the wild-type, but differentially methylated, p21 allele.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Baylin SB, Esteller M, Rountree MR, Bachman KE, Schuebel K and Herman JG . (2001). Hum. Mol. Genet., 10, 687–692.
Boolbol SK, Dannenberg AJ, Chadburn A, Martucci C, Guo XJ, Ramonetti JT, Abreu-Goris M, Newmark HL, Lipkin ML, DeCosse JJ and Bertagnolli MM . (1996). Cancer Res., 56, 2556–2560.
Christman JK . (2002). Oncogene, 21, 5483–5495.
Davis CD and Uthus EO . (2003). J. Nutr., 133, 2907–2914.
Deng C, Zhang P, Harper JW, Elledge SJ and Leder P . (1995). Cell, 82, 675–684.
el-Deiry WS, Tokino T, Waldman T, Oliner JD, Velculescu VE, Burrell M, Hill DE, Healy E, Rees JL and Hamilton SR . (1995). Cancer Res., 55, 2910–2919.
Esteller M . (2002). Oncogene, 21, 5427–5440.
Esteller M, Fraga MF, Paz MF, Campo E, Colomer D, Novo FJ, Calasanz MJ, Galm O, Guo M, Benitez J and Herman JG . (2002). Science, 297, 1807–1808 discussion 1807–1808.
Fodde R, Edelmann W, Yang K, van Leeuwen C, Carlson C, Renault B, Breukel C, Alt E, Lipkin M, Khan PM and Kucherlapati R . (1994). Proc. Natl. Acad. Sci. USA, 91, 8969–8973.
Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, Molloy PL and Paul CL . (1992). Proc. Natl. Acad. Sci. USA, 89, 1827–1831.
Gartel AL and Tyner AL . (1999). Exp. Cell Res., 246, 280–289.
Giardiello FM, Hamilton SR, Krush AJ, Piantadosi S, Hylind LM, Celano P, Booker SV, Robinson CR and Offerhaus GJ . (1993). N. Engl. J. Med., 328, 1313–1316.
Goldberg Y, Nassif II, Pittas A, Tsai LL, Dynlacht BD, Rigas B and Shiff SJ . (1996). Oncogene, 12, 893–901.
Jones PA and Baylin SB . (2002). Nat. Rev. Genet., 3, 415–428.
Kondo Y, Shen L and Issa JP . (2003). Mol. Cell. Biol., 23, 206–215.
Kondo Y, Shen L, Yan PS, Huang TH and Issa JP . (2004). Proc. Natl. Acad. Sci. USA, 101, 7398–7403.
Labayle D, Fischer D, Vielh P, Drouhin F, Pariente A, Bories C, Duhamel O, Trousset M and Attali P . (1991). Gastroenterology, 101, 635–639.
Laird PW . (2003). Nat. Rev. Cancer, 3, 253–266.
Lavelle D, DeSimone J, Hankewych M, Kousnetzova T and Chen YH . (2003). Leuk. Res., 27, 999–1007.
Li LC and Dahiya R . (2002). Bioinformatics, 18, 1427–1431.
Nugent KP, Farmer KC, Spigelman AD, Williams CB and Phillips RK . (1993). Br. J. Surg., 80, 1618–1619.
Pohlmann P, DiLeone LP, Cancella AI, Caldas AP, Dal Lago L, Campos Jr O, Monego E, Rivoire W and Schwartsmann G . (2002). Am. J. Clin. Oncol., 25, 496–501.
Polyak K, Hamilton SR, Vogelstein B and Kinzler KW . (1996). Am. J. Pathol., 149, 381–387.
Pretlow TP, Edelmann W, Kucherlapati R, Pretlow TG and Augenlicht LH . (2003). Am. J. Pathol., 163, 1757–1763.
Rountree MR, Bachman KE, Herman JG and Baylin SB . (2001). Oncogene, 20, 3156–3165.
Shiff SJ and Rigas B . (1997). Gastroenterology, 113, 1992–1998.
Soh JW and Weinstein IB . (2003). Prog. Exp. Tumor Res., 37, 261–285.
Subbaramaiah K, Zakim D, Weksler BB and Dannenberg AJ . (1997). Proc. Soc. Exp. Biol. Med., 216, 201–210.
Toyota M, Ahuja N, Ohe-Toyota M, Herman JG, Baylin SB and Issa JP . (1999). Proc. Natl. Acad. Sci. USA, 96, 8681–8686.
Toyota M and Issa JP . (1999). Semin. Cancer Biol., 9, 349–357.
van de Wetering M, Sancho E, Verweij C, de Lau W, Oving I, Hurlstone A, van der Horn K, Batlle E, Coudreuse D, Haramis AP, Tjon-Pon-Fong M, Moerer P, van den Born M, Soete G, Pals S, Eilers M, Medema R and Clevers H . (2002). Cell, 111, 241–250.
Waddell WR, Ganser GF, Cerise EJ and Loughry RW . (1989). Am. J. Surg., 157, 175–179.
Xian J, Aitchison A, Bobrow L, Corbett G, Pannell R, Rabbitts T and Rabbitts P . (2004). Cancer Res., 64, 6432–6437.
Yang WC, Bancroft L, Nicholas C, Lozonschi I and Augenlicht LH . (2003). Cancer Res., 63, 4990–4996.
Yang WC, Mathew J, Velcich A, Edelmann W, Kucherlapati R, Lipkin M, Yang K and Augenlicht LH . (2001a). Cancer Res., 61, 565–569.
Yang WC, Velcich A, Mariadason J, Nicholas C, Corner G, Houston M, Edelmann W, Kucherlapati R, Holt PR and Augenlicht LH . (2001b). Cancer Res., 61, 6297–6302.
Zhu WG, Hileman T, Ke Y, Wang P, Lu S, Duan W, Dai Z, Tong T, Villalona-Calero MA, Plass C and Otterson GA . (2004). J. Biol. Chem., 279, 15161–15166.
Acknowledgements
We wish to acknowledge support of the Albert Einstein Cancer Center Core DNA-sequencing facility, and our thanks to Dr Philip Leder for providing the p21 knockout mice and to Min Zhuang for the assistance of statistic analysis. Grant support: This work was supported in part by CA96605, CA120196, CA87559, and PO1 13330 from the National Cancer Institute.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yang, W., Bancroft, L. & Augenlicht, L. Methylation in the p21WAF1/cip1 promoter of Apc+/−, p21+/− mice and lack of response to sulindac. Oncogene 24, 2104–2109 (2005). https://doi.org/10.1038/sj.onc.1208444
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1208444
Keywords
This article is cited by
-
Epigenetic modifications of the immune system in health and disease
Immunology & Cell Biology (2015)
-
Commensal microbiota regulates T cell fate decision in the gut
Seminars in Immunopathology (2015)
-
The epigenetic regulator Uhrf1 facilitates the proliferation and maturation of colonic regulatory T cells
Nature Immunology (2014)
-
Selenium and sulindac are synergistic to inhibit intestinal tumorigenesis in Apc/p21 mice
Journal of Hematology & Oncology (2013)