Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Identification of RASSF8 as a candidate lung tumor suppressor gene

Oncogene volume 25, pages 3934–3938 (2006)Cite this article

Abstract

The RASSF8 gene, which maps close to the KRAS2 gene, contains a RAS-associated domain and encodes a protein that is evolutionarily conserved from fish to humans. Analysis of the RASSF8 transcript revealed a complex expression pattern of 5′-UTR mRNA isoforms in normal lung and in lung adenocarcinomas (ADCAs), with no apparent differences. However, RASSF8 gene transcript levels were ∼seven-fold-lower in lung ADCAs as compared to normal lung tissue. Expression of RASSF8 protein by transfected lung cancer cells led to inhibition of anchorage-independent growth in soft agar in A549 cells and reduction of clonogenic activity in NCI-H520 cells. These results raise the possibility protein encoded by RASSF8 is a novel tumor suppressor for lung cancer.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

Accession codes

Accessions

GenBank/EMBL/DDBJ

References

  • Campbell PM, Der CJ . (2004). Semin Cancer Biol 14: 105–114.

  • Clezardin P . (1998). Cell Mol Life Sci 54: 541–548.

  • Dammann R, Li C, Yoon JH, Chin PL, Bates S, Pfeifer GP . (2000). Nat Genet 25: 315–319.

  • De Gregorio L, Manenti G, Incarbone M, Pilotti S, Pastorino U, Pierotti MA et al. (1998). Int J Cancer 79: 269–272.

  • Debeer P, Schoenmakers EF, Twal WO, Argraves WS, De Smet L, Fryns JP et al. (2002). J Med Genet 39: 98–104.

  • Ellis CA, Clark G . (2000). Cell Signal 12: 425–434.

  • Guerois R, Nielsen JE, Serrano L . (2002). J Mol Biol 320: 369–387.

  • Hesson L, Dallol A, Minna JD, Maher ER, Latif F . (2003). Oncogene 22: 947–954.

  • Huncharek M, Muscat J, Geschwind JF . (1999). Carcinogenesis 20: 1507–1510.

  • Jiang Y, Cui L, Yie TA, Rom WN, Cheng H, Tchou-Wong KM . (2001). Oncogene 20: 2254–2263.

  • Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, Tuveson DA et al. (2001). Nature 410: 1111–1116.

  • Kiel C, Wohlgemuth S, Rousseau F, Schymkowitz J, Ferkinghoff-Borg J, Wittinghofer F et al. (2005). J Mol Biol 348: 759–775.

  • Malaney S, Daly RJ . (2001). J Mammary Gland Biol Neoplasia 6: 101–113.

  • Malumbres M, Barbacid M . (2003). Nat Rev Cancer 3: 459–465.

  • Mascaux C, Iannino N, Martin B, Paesmans M, Berghmans T, Dusart M et al. (2005). Br J Cancer 92: 131–139.

  • Nakanishi K, Sakamoto M, Yasuda J, Takamura M, Fujita N, Tsuruo T et al. (2002). Cancer Res 62: 2971–2975.

  • Nikiforov MA, Hagen K, Ossovskaya VS, Connor TM, Lowe SW, Deichman GI et al. (1996). Oncogene 13: 1709–1719.

  • Wall JD, Pritchard JK . (2003). Nat Rev Genet 4: 587–597.

  • Wohlgemuth S, Kiel C, Kramer A, Serrano L, Wittinghofer F, Herrmann C . (2005). J Mol Biol 348: 741–758.

Download references

Acknowledgements

We thank Christina Kiel for the in silico analysis of the RAS binding domain of the RASSF8 protein. This work was funded in part by grants from Associazione and Fondazione Italiana Ricerca Cancro (AIRC and FIRC) and from FIRB. GenBank Acc. Numbers: AY665468 to AY665471.

Author information

Authors and Affiliations

  1. Department of Experimental Oncology and Laboratories, Istituto Nazionale Tumori, Milan, Italy

    F S Falvella, G Manenti, M Spinola, C Pignatiello & T A Dragani

  2. Unit of Thoracic Surgery, Istituto Nazionale Tumori, Milan, Italy

    B Conti & U Pastorino

Authors
  1. F S Falvella
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G Manenti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M Spinola
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C Pignatiello
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B Conti
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. U Pastorino
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T A Dragani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T A Dragani.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Falvella, F., Manenti, G., Spinola, M. et al. Identification of RASSF8 as a candidate lung tumor suppressor gene. Oncogene 25, 3934–3938 (2006). https://doi.org/10.1038/sj.onc.1209422

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1209422

Keywords

This article is cited by

Search

Advanced search

Quick links