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Prognosis of stage II colon cancer by non-neoplastic mucosa gene expression profiling

Oncogene volume 26, pages 2642–2648 (2007)Cite this article

Abstract

We have assessed the possibility to build a prognosis predictor (PP), based on non-neoplastic mucosa microarray gene expression measures, for stage II colon cancer patients. Non-neoplastic colonic mucosa mRNA samples from 24 patients (10 with a metachronous metastasis, 14 with no recurrence) were profiled using the Affymetrix HGU133A GeneChip. Patients were repeatedly and randomly divided into 1000 training sets (TSs) of size 16 and validation sets (VS) of size 8. For each TS/VS split, a 70-gene PP, identified on the TS by selecting the 70 most differentially expressed genes and applying diagonal linear discriminant analysis, was used to predict the prognoses of VS patients. Mean prognosis prediction performances of the 70-gene PP were 81.8% for accuracy, 73.0% for sensitivity and 87.1% for specificity. Informative genes suggested branching signal-transduction pathways with possible extensive networks between individual pathways. They also included genes coding for proteins involved in immune surveillance. In conclusion, our study suggests that one can build an accurate PP for stage II colon cancer patients, based on non-neoplastic mucosa microarray gene expression measures.

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Acknowledgements

The first author (Alain Barrier) has received a grant of the Ligue Nationale Contre le Cancer. The work has been supported by the California Institute for Quantitative Biomedical Research (QB3), UC Berkeley.

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Authors and Affiliations

  1. Service de Chirurgie Digestive, Hôpital Tenon, AP-HP, Paris, France

    A Barrier, F Lacaine & S Houry

  2. UMR S 707, INSERM, Paris, France

    A Barrier, P-Y Boëlle & A Flahault

  3. Faculté de Médecine, Université Pierre et Marie Curie-Paris 6, Paris, France

    A Barrier, P-Y Boëlle, C Tse, D Brault, F Lacaine, S Houry, P Callard & A Flahault

  4. Division of Biostatistics, School of Public Health, University of California, Berkeley, CA, USA

    A Barrier & S Dudoit

  5. UMR S 602, INSERM, Villejuif, France

    F Roser, B Debuire & A Lemoine

  6. Service de Biochimie, Hôpital Paul Brousse, AP-HP, Villejuif, France

    F Roser, B Debuire & A Lemoine

  7. Faculté de Médecine, Université Paris 11, Villejuif, France

    F Roser, B Debuire & A Lemoine

  8. Service d'Anatomie Pathologique, Hôpital Ambroise Paré, AP-HP, Boulogne Billancourt, France

    B Franc

  9. Faculté de Médecine, Université Versailles Saint Quentin, Boulogne Billancourt, France

    B Franc & C Penna

  10. Service de Biochimie, Hôpital Tenon, AP-HP, Paris, France

    C Tse & D Brault

  11. Service d'Anatomie Pathologique, Hôpital Tenon, AP-HP, Paris, France

    P Callard

  12. Service de Chirurgie Digestive, Hôpital Ambroise Paré, AP-HP, Boulogne Billancourt, France

    C Penna

Authors
  1. A Barrier
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  2. F Roser
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  5. C Tse
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  6. D Brault
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  7. F Lacaine
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  8. S Houry
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  11. B Debuire
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  12. A Flahault
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  13. S Dudoit
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  14. A Lemoine
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Corresponding author

Correspondence to A Lemoine.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Barrier, A., Roser, F., Boëlle, PY. et al. Prognosis of stage II colon cancer by non-neoplastic mucosa gene expression profiling. Oncogene 26, 2642–2648 (2007). https://doi.org/10.1038/sj.onc.1210060

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  • DOI: https://doi.org/10.1038/sj.onc.1210060

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