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Differential motility of p190bcr-abl- and p210bcr-abl-expressing cells: respective roles of Vav and Bcr-Abl GEFs

Oncogene volume 27, pages 2673–2685 (2008)Cite this article

Abstract

The chimeric oncogene Bcr-Abl is known to induce autonomous motility of leukemic cells. We show here that p210bcr-abl responsible for chronic myelogenous leukemia induces an amoeboid type of motility while p190bcr-abl, associated with acute lymphoid leukemia, induces a rolling type of motility. We previously reported that p210bcr-abl activates RhoA and Rac1, while p190bcr-abl although devoid of a Dbl-homology (DH) domain activates Rac1, but not RhoA. We investigated the regulation of GDP/GTP exchange factor (GEF) activities in the Bcr-Abl complex. For that purpose, different GEF activity mutants of Vav and of Bcr-Abl were constructed and stably transfected in Ba/F3 cells. Using these mutants, we demonstrate that RhoA is exclusively activated by the DH domain of p210bcr-abl, while Rac1 activation is mostly due to Vav. Inhibition of Rac1 by Vav GEF mutant leads to immobilization of cells. Vav depletion using shRNA also induces immobilization of cells and suppression of GTP-bound Rac1. RhoA inactivation induces the specific loss of amoeboid movements. These results suggest that Rac1 activation by Vav triggers the motility of Bcr-Abl-expressing Ba/F3 cells, while the specific amoeboid mode of motility induced by p210bcr-abl is a consequence of RhoA activation.

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Acknowledgements

We are grateful to Dr Michel Popoff (Unité des Toxines Microbiennes, Institut Pasteur, Paris) for providing us with Ia-C3/Ib. Many thanks to Dr Anne Cantereau for helpful and efficient confocal station technical assistance. We also thank Maria Barreira-Gonzalez and Mélanie Magnan for technical assistance, Pr Jean-Marc Gombert and Angélique Chavineau for FACS analysis and Dr Sylvie Chevalier (Inserm U564, Angers) for providing the Amaxa transfection device. This work was in part supported by grants provided by the ‘Ligue Nationale contre le Cancer, Charente-maritime, Charente and Vienne’. Thomas Daubon is recipient of a fellowship from MENRT. This manuscript is dedicated to the memory of Professor Pierre V Vignais.

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Authors and Affiliations

  1. IPBC, CNRS UMR 6187, Université de Poitiers, Poitiers, France

    T Daubon, J Chasseriau, A El Ali, A Kitzis, B Constantin & N Bourmeyster

  2. LGCM, CHU de Poitiers, BP 577, Poitiers, France

    J Rivet, A Kitzis & N Bourmeyster

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  1. T Daubon
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  2. J Chasseriau
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Correspondence to N Bourmeyster.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Daubon, T., Chasseriau, J., Ali, A. et al. Differential motility of p190bcr-abl- and p210bcr-abl-expressing cells: respective roles of Vav and Bcr-Abl GEFs. Oncogene 27, 2673–2685 (2008). https://doi.org/10.1038/sj.onc.1210933

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