Issue 3, 2013
From the journal:

Analytical Methods

Determination of aristolochic acids in rat serum by high performance liquid chromatography-Q-TOF tandem mass spectrometry†

Jiangping Gu,a   Pinjie Zhang,a   Feifang Zhang,*a   HeQing Shen,b   Bingcheng Yang,*a   Xinmiao Lianga  and  Xiaogang Chuc  

* Corresponding authors

a Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
E-mail: zhangff@ecust.edu.cn
Fax: +86 21-64250627
Tel: +86 21-64250627

b Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China

c Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China

Abstract

Four aristolochic acids (AAs) including AA-I, AA-II, AA-IIIa and AA-IVa in rat serum were simultaneously determined by HPLC-Q-TOF-MS. A new solid phase extraction (SPE) resin with reversed-phase/strong anion exchange mixed groups (C18-SAX) was used to effectively purify and enrich the analytes, which demonstrated much better performance in comparison with common C18-SPE resin in terms of noise level and recovery. The chromatographic separation was performed on a C18 column under isocratic elution, and the effluent was analyzed by TOF-MS-MS in the mode of positive multiple reaction. Naproxen was used as the internal standard (IS). The limit of detection was 3, 6, 0.09 and 1.5 ng mL−1 for AA-I, AA-II, AA-IIIa and AA-IVa, respectively. Intra- and inter-day precisions measured as relative standard deviation of retention time were less than 1.1% and 1.5%, respectively.

Graphical abstract: Determination of aristolochic acids in rat serum by high performance liquid chromatography-Q-TOF tandem mass spectrometry

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C2AY25860A
Article type
Paper
Submitted
05 Aug 2012
Accepted
07 Nov 2012
First published
12 Nov 2012

Anal. Methods, 2013,5, 718-721

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Determination of aristolochic acids in rat serum by high performance liquid chromatography-Q-TOF tandem mass spectrometry

J. Gu, P. Zhang, F. Zhang, H. Shen, B. Yang, X. Liang and X. Chu, Anal. Methods, 2013, 5, 718 DOI: 10.1039/C2AY25860A

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