Issue 8, 2001

Biochemically functionalized silica nanoparticles

Abstract

In this report, we demonstrate the biochemical modification of silica based nanoparticles. Both pure and dye-doped silica nanoparticles were prepared, and their surfaces were modified with enzymes and biocompatible chemical reagents that allow them to function as biosensors and biomarkers. The nanoparticles produced in this work are uniform in size with a 1.6% relative standard deviation. They have a pure silica surface and can thus be modified easily with many biomolecules for added biochemical functionality. Specifically, we have modified the nanoparticle surfaces with enzyme molecules (glutamate dehydrogenase (GDH) and lactate dehydrogenase (LDH)) and a biocompatible reagent for cell membrane staining. Experimental results show that the silica nanoparticles are a good biocompatible solid support for enzyme immobilization. The immobilized enzyme molecules on the nanoparticle surface have shown excellent enzymatic activity in their respective enzymatic reactions. The nanoparticle surface biochemical functionalization demonstrates the feasibility of using nanoparticles for biosensing and biomarking applications.

Article information

Article type
Paper
Submitted
15 Feb 2001
Accepted
21 May 2001
First published
20 Jul 2001

Analyst, 2001,126, 1274-1278

Biochemically functionalized silica nanoparticles

M. Qhobosheane, S. Santra, P. Zhang and W. Tan, Analyst, 2001, 126, 1274 DOI: 10.1039/B101489G

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