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Lethal photosensitisation of oral bacteria and its potential application in the photodynamic therapy of oral infections

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Abstract

Chemical antibacterial agents are increasingly being used in prophylactic and therapeutic regimes for dental plaque-related diseases, which are among the most common human infections. As these agents are difficult to maintain at a therapeutic concentration in the oral cavity and can be rendered ineffective by resistance development in the target organisms, there is a need to develop alternative antimicrobial approaches. Bacteria and other microbes can be sensitised to light through prior treatment with a chemical photosensitising agent. Lethal photosensitisation of a wide range of bacteria responsible for caries, periodontal diseases and root canal infections has been demonstrated using red light in conjunction with a number of photosensitisers, including Toluidine Blue, phthalocyanines and chlorins. The advantages of this approach are that bacteria can be eradicated in very short periods of time (seconds or minutes), resistance development in the target bacteria is unlikely and damage to adjacent host tissues and disruption of the normal microflora can be avoided. This approach may be a useful alternative to antibiotics and antiseptics in eliminating cariogenic and periodontopathogenic bacteria from disease lesions and for the disinfection of root canals. Not only would this be of benefit for the treatment of these diseases but, by replacing the antimicrobial agents that are currently used for such purposes, it would help to conserve our dwindling supply of antimicrobial agents that are effective in the treatment of serious systemic infections.

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References

  1. G. S. Schuster, Oral flora and pathogenic organisms, Infect. Dis. Clin. North Am., 1999, 13, 757–774.

    Article  CAS  PubMed  Google Scholar 

  2. E. Kononen, Development of oral bacterial flora in young children, Ann. Med., 2000, 32, 107–112.

    Article  CAS  PubMed  Google Scholar 

  3. B. Henderson and M. Wilson, Commensal communism and the oral cavity, J. Dent. Res., 1998, 77, 1674–1683.

    Article  CAS  PubMed  Google Scholar 

  4. D. H. Meyer and P. M. Fives-Taylor, Oral pathogens: from dental plaque to cardiac disease, Curr. Opin. Microbiol., 1998, 1, 88–95.

    Article  CAS  PubMed  Google Scholar 

  5. M. Wilson, Bacterial biofilms and human disease, Sci. Prog., 2001, 84, 235–254.

    Article  CAS  PubMed  Google Scholar 

  6. H. N. Newman and M. Wilson, Dental Plaque Revisited: Oral Biofilms in Health and Disease, BioLine, Cardiff, 1999.

    Google Scholar 

  7. Oral Health in America: A Report of the Surgeon General, National Institute of Dental and Craniofacial Research, National Institutes of Health, US Department of Health and Human Services, Rockville, MD, 2000.

  8. B. A. Burt and S. A. Eklund, Dentistry, Dental Practice, and the Community, W. B. Saunders Co., Philadelphia, 1999.

    Google Scholar 

  9. S. Joyston-Bechal and E. A. Kidd, The effect of three commercially available saliva substitutes on enamel in vitro, Br. Dent. J., 1987, 163, 187–190.

    Article  CAS  PubMed  Google Scholar 

  10. M. Balakrishnan, R. S. Simmonds and J. R. Tagg, Dental caries is a preventable infectious disease, Aust. Dent. J., 2000, 45, 235–245.

    Article  CAS  PubMed  Google Scholar 

  11. W. J. Loesche and N. S. Grossman, Periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment, Clin. Microbiol. Rev., 2001, 14, 727–752.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. G. Suchett-Kaye, J. J. Morrier and O. Barsotti, Clinical usefulness of microbiological diagnostic tools in the management of periodontal disease, Res. Microbiol., 2001, 152, 631–639.

    Article  CAS  PubMed  Google Scholar 

  13. M. Quirynen, W. Teughels, M. De Soete and D. van Steenberghe, Topical antiseptics and antibiotics in the initial therapy of chronic adult periodontitis: microbiological aspects, Periodontol 2000, 2002, 28, 72–90.

    Article  PubMed  Google Scholar 

  14. M. G. Jorgensen and J. Slots, Responsible use of antimicrobials in periodontics, J. Calif. Dent. Assoc., 2000, 28, 185–193.

    CAS  PubMed  Google Scholar 

  15. P. W. Caufield, A. P. Dasanayake and Y. Li, The antimicrobial approach to caries management, J. Dent. Educ., 2001, 65, 1091–1095.

    Article  CAS  PubMed  Google Scholar 

  16. L. Lopez, R. Berkowitz, C. Spiekerman and P. Weinstein, Topical antimicrobial therapy in the prevention of early childhood caries: a follow-up report, Pediatr. Dent., 2002, 24, 204–206.

    PubMed  Google Scholar 

  17. H. Martin, Cleanliness, disinfection, and sterilization of the root canal, Curr. Opin. Dent., 1991, 1, 734–746.

    CAS  PubMed  Google Scholar 

  18. M. Wainwright, Photodynamic antimicrobial chemotherapy (PACT), J. Antimicrob. Chemother., 1998, 42, 13–28.

    Article  CAS  PubMed  Google Scholar 

  19. M. Wilson, Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal disease: a review, J. Appl. Bacteriol., 1993, 75, 299–306.

    Article  CAS  PubMed  Google Scholar 

  20. M. Wilson, Bactericidal effect of laser light and its potential use in the treatment of plaque-related diseases, Int. Dent. J., 1994, 44, 181–189.

    CAS  PubMed  Google Scholar 

  21. P. D. Marsh, Microbiologic aspects of dental plaque and dental caries, Dent. Clin. North Am., 1999, 43, 599–614.

    CAS  PubMed  Google Scholar 

  22. R. M. Joharji and J. O. Adenubi, Prevention of pit and fissure caries using an antimicrobial varnish: 9 month clinical evaluation, J. Dent., 2001, 29, 247–254.

    Article  CAS  PubMed  Google Scholar 

  23. J. B. Summitt, Conservative cavity preparations, Dent. Clin. North Am., 2002, 46, 171–184.

    Article  PubMed  Google Scholar 

  24. M. Wilson, J. Dobson and W. Harvey, Sensitisation of oral bacteria to killing by low-power laser radiation, Curr. Microbiol., 1992, 25, 77–81.

    Article  CAS  PubMed  Google Scholar 

  25. T. Burns, M. Wilson and G. Pearson, Sensitisation of cariogenic bacteria to killing by light from a helium/neon laser, J. Med. Microbiol., 1993, 38, 401–405.

    Article  CAS  PubMed  Google Scholar 

  26. T. Burns, M. Wilson and G. Pearson, Killing of cariogenic bacteria by light from a gallium arsenide diode laser, J. Dent., 1994, 22, 273–278.

    Article  CAS  PubMed  Google Scholar 

  27. T. Burns, M. Wilson and G. J. Pearson, Mechanism of killing of Streptococcus mutans by light-activated drugs, Proc. SPIE-Int. Soc. Opt. Eng., 1995, 2625, 288–297.

    Google Scholar 

  28. M. Wilson, T. Burns and J. Pratten and G. J. Pearson, Bacteria in supragingival plaque samples can be killed by low-power laser light in the presence of a photosensitiser, J. Appl. Bacteriol., 1995, 78, 569–574.

    Article  CAS  PubMed  Google Scholar 

  29. M. Wilson, T. Burns and J. Pratten, Killing of Streptococcus sanguis in biofilms using a light-activated antimicrobial agent, J. Antimicrob. Chemother., 1996, 37, 377–381.

    Article  CAS  PubMed  Google Scholar 

  30. M. Wilson, Control of oral biofilms by light-activated antimicrobial agents, in Oral Biofilms and Plaque Control, ed. H. J. Busscher and L. V. Evans, Harwood Academic, Amsterdam, 1999, pp. 323–340.

    Google Scholar 

  31. S. Wood, B. Nattress, J. Kirkham, R. Shore, S. Brookes, J. Griffiths and C. Robinson, An in vitro study of the use of photodynamic therapy for the treatment of natural oral plaque biofilms formed in vivo, J. Photochem. Photobiol., B., 1999, 50, 1–7.

    Article  CAS  Google Scholar 

  32. T. Burns, M. Wilson and G. Pearson, Effect of dentine and collagen on the lethal photosensitisation of Streptococcus mutans, Caries Res., 1995, 29, 192–197.

    Article  CAS  PubMed  Google Scholar 

  33. D. F. Kinane and D. F. Lappin, Clinical, pathological and immunological aspects of periodontal disease, Acta Odontol. Scand., 2001, 59, 154–160.

    Article  CAS  PubMed  Google Scholar 

  34. D. F. Kinane, Causation and pathogenesis of periodontal disease, Periodontol 2000, 2001, 25, 8–20.

    Article  CAS  PubMed  Google Scholar 

  35. J. Travis, A. Banbula and J. Potempa, The role of bacterial and host proteinases in periodontal disease, Adv. Exp. Med. Biol., 2000, 477, 455–465.

    Article  CAS  PubMed  Google Scholar 

  36. M. A. Curtis, J. Aduse-Opoku and M. Rangarajan, Cysteine proteases of Porphyromonas gingivalis, Crit. Rev. Oral Biol. Med., 2001, 12, 192–216.

    Article  CAS  PubMed  Google Scholar 

  37. M. Wilson, R. Seymour and B. Henderson, Bacterial perturbation of cytokine networks, Infect. Immun., 1998, 66, 2401–2409.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. M. Wilson and B. Henderson, Cytokine induction by periodontopathogenic bacteria, J. Immunol. Immunopharm., 1998, 18, 35–42.

    CAS  Google Scholar 

  39. G. J. Seymour and E. Gemmell, Cytokines in periodontal disease: where to from here?, Acta. Odontol. Scand., 2001, 59, 167–173.

    Article  CAS  PubMed  Google Scholar 

  40. D. T. Graves, The potential role of chemokines and inflammatory cytokines in periodontal disease progression, Clin. Infect. Dis., 1999, 28, 482–490.

    Article  CAS  PubMed  Google Scholar 

  41. K. Schwach-Abdellaoui, N. Vivien-Castioni and R. Gurny, Local delivery of antimicrobial agents for the treatment of periodontal diseases, Eur. J. Pharm. Biopharm., 2000, 50, 83–99.

    Article  CAS  PubMed  Google Scholar 

  42. D. F. Kinane, Local antimicrobial therapies in periodontal disease, Ann. R. Australas. Coll. Dent. Surg., 2000, 15, 57–60.

    CAS  PubMed  Google Scholar 

  43. M. Wilson, J. Dobson and S. Sarkar, Sensitisation of periodontopathogenic bacteria to killing by light from a low-power laser, Oral Microbiol. Immunol., 1993, 8, 182–187.

    Article  CAS  PubMed  Google Scholar 

  44. S. Sarkar and M. Wilson, Lethal photosensitisation of bacteria in subgingival plaque samples from patients with chronic periodontitis, J. Periodont. Res., 1993, 28, 204–210.

    Article  CAS  Google Scholar 

  45. C. R. Rovaldi, A. Pievsky, N. A. Sole, P. M. Friden, D. M. Rothstein and P. Spacciapoli, Photoactive porphyrin derivative with broad-spectrum activity against oral pathogens in vitro, Antimicrob. Agents Chemother., 2000, 44, 3364–3367.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. M. Wilson, S. Sarkar and J. Bulman, Effect of blood on the lethal photosensitization of bacteria in subgingival plaque from patients with chronic periodontitis, Lasers Med. Sci., 1994, 8, 297–303.

    Article  Google Scholar 

  47. M. Wilson, Biological activities of lipopolysaccharides from oral bacteria and their relevance to the pathogenesis of chronic periodontitis, Sci. Prog., 1995, 78, 19–34.

    CAS  PubMed  Google Scholar 

  48. N. Kömerik, M. Wilson and S. Poole, The effect of photodynamic action on two virulence factors of Gram-negative bacteria, Photochem. Photobiol., 2000, 72, 676–680.

    Article  PubMed  Google Scholar 

  49. S. Packer, M. Bhatti, T. Burns and M. Wilson, Inactivation of proteolytic enzymes from Porphyromonas gingivalis using light-activated agents, Lasers Med. Sci., 2000, 15, 24–30.

    Article  CAS  PubMed  Google Scholar 

  50. N. Kömerik, A. Curnow, A. J. MacRobert, C. Hopper, P. M. Speight and M. Wilson, Fluorescence biodistribution and photosensitising activity of toluidine blue O on rat buccal mucosa, Lasers Med. Sci., 2002, 17, 86–92.

    Article  PubMed  Google Scholar 

  51. C. E. Millson, M. Wilson, A. J. MacRobert and S. G. Bown, Ex-vivo treatment of gastric Helicobacter infection by photodynamic therapy, J. Photochem. Photobiol., B, 1996, 32, 59–65.

    Article  CAS  Google Scholar 

  52. N. S. Soukos, L. A. Ximenez-Fyvie, M. R. Hamblin, S. S. Socransky and T. Hasan, Targeted antimicrobial photochemotherapy, Antimicrob. Agents Chemother., 1998, 42, 2595–2601.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. M. Bhatti, A. MacRobert, B. Henderson, P. Shepherd, J. Cridland and M. Wilson, Antibody-targeted lethal photosensitisation of Porphyromonas gingivalis, Antimicrob. Agents Chemother., 2000, 44, 2615–2618.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. P. R. N. Nair, Light and electron microscope studies of root canal flora and periapical lesions, J. Endodontol., 1987, 13, 29–39.

    Article  Google Scholar 

  55. D. A. Spratt, J. Pratten, M. Wilson and K. Gulabivala, An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates, Int. Endodontol. J., 2001, 34, 300–307.

    Article  CAS  Google Scholar 

  56. Resistance to Antibiotics and Other Antimicrobial Agents. 7th Report from the House of Lords Science and Technology Committee: Session 1997–98, HM Stationery Office, London, 1998.

  57. The Path of Least Resistance, Standing Medical Advisory Committee, UK Department of Health, London, 1998.

  58. Opinion of the Scientific Steering Committee on Antimicrobial Resistance, European Commission, Brussels, 1999.

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Wilson, M. Lethal photosensitisation of oral bacteria and its potential application in the photodynamic therapy of oral infections. Photochem Photobiol Sci 3, 412–418 (2004). https://doi.org/10.1039/b211266c

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