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Targeted photodynamic therapy of breast cancer cells using antibody-phthalocyanine-gold nanoparticle conjugates

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Abstract

A 4-component antibody-phthalocyanine-polyethylene glycol-gold nanoparticle conjugate is described for use as a potential drug for targeted photodynamic cancer therapy. Gold nanoparticles (4 nm) were stabilised with a self-assembled layer of a zinc-phthalocyanine derivative (photosensitiser) and a heterobifunctional polyethylene glycol. Anti-HER2 monoclonal antibodies were covalently bound to the nanoparticles via a terminal carboxy moiety on the polyethylene glycol. The nanoparticle conjugates were stable towards aggregation, and under irradiation with visible red light efficiently produced cytotoxic singlet oxygen. Cellular experiments demonstrated that the nanoparticle conjugates selectively target breast cancer cells that overexpress the HER2 epidermal growth factor cell surface receptor, and that they are effective photodynamic therapy agents.

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Authors and Affiliations

  1. School of Chemistry, University of East Anglia, Norwich, Norfolk, NR4 7TJ, UK

    Tanya Stuchinskaya, Miguel Moreno, Michael J. Cook & David A. Russell

  2. School of Biological Sciences, University of East Anglia, Norwich, Norfolk, NR4 7TJ, UK

    Dylan R. Edwards

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  1. Tanya Stuchinskaya
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  2. Miguel Moreno
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Correspondence to David A. Russell.

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This article is published as part of a themed issue on immunological aspects and drug delivery technologies in PDT.

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Stuchinskaya, T., Moreno, M., Cook, M.J. et al. Targeted photodynamic therapy of breast cancer cells using antibody-phthalocyanine-gold nanoparticle conjugates. Photochem Photobiol Sci 10, 822–831 (2011). https://doi.org/10.1039/c1pp05014a

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